Hybridization
Fluorescence Image Analyzer - FLIMA: software for quantitative analysis of fluorescence in situ hybridization
Authors: H.C.M. Silva, M.M.C. Martins-J�ºnior, L.B. Ribeiro, D.A. Matoso
The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the a.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019658
- DOI:
- 10.4238/gmr16019658
Replication timing regulation in adults with chromosomal balance rearrangements
Authors: Y. Xie, J. Wu, S. Lin, Y. Zhou, Q. Fang and X. Sun
The alternative forms of the alleles in biallelic genes display a synchronous pattern of replication that is different from genes subjected to monoallelic expression, which exhibit an asynchronous mode of replication. The present study sought to gain insight into changes in the allele-specific replication timing in phenotypically normal humans with balanc.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.9
- DOI:
- 10.4238/2015.July.14.9
Novel genetic male sterility developed in (Capsicum annuum x C. chinense) x C. pubescens and induced by HNO2 showin Mendelian inheritance and aborted at telophase of microspore mother cell stage
Authors: W. Huang, J.-J. Ji, C. Li, G.-Q. Li, C.-C. Yin, W.-G. Chai and Z.-H. Gong
A novel genetic male sterile germplasm was developed by successively crossing of (C. annuum x C. chinense) x C. pubescens and by chemical mutagenesis in pepper. The sterile anthers showed morphological abnormalities, but pistils developed normally with fine pollination capability. We investigated fertility segregation through sib-crossing of the same stra.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.13.11
- DOI:
- http://dx.doi.org/10.4238/2015.April.13.11
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora
Authors: C.A.F. Melo, G.S. Silva and M.M. Souza
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prep.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.4
- DOI:
- 10.4238/2015.March.27.4
Fluorescence in situ hybridization analysis with subtelomere specific probes (12pter-15qter) showed no differences in deletion patterns between normotensive and essential hypertension
Authors: S.T. Onrat and A.G. Tomat�±r
Telomere biology is intimately linked to the genetic/environmental etiology of cardiovascular and metabolic diseases and telomere shortening is emerging as an important biomarker disease. The relationship between subtelomeric deletions and genetic hypertension was examined. Fluorescence in situ hybridization was used to direct.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3gmr464
- DOI:
- 10.4238/vol7-3gmr464
Localization of rDNA sites in holocentric chromosomes of three species of triatomines (Heteroptera, Triatominae)
Authors: Grasiela Dias de Campos Severi-Aguiar and Maria TercÃ?Âlia Vilela de Azeredo-Oliveira
Chromatin organization in the holocentric chromosomes of three triatomines species was cytologically studied by fluorescent in situ hybridization with a 45S rDNA probe of Drosophila melanogaster to localize ribosomal genes. In Triatoma tibiamaculata, metaphases I showed telomeric highlights in a single, larger bivalent. In T. .. Read More»
- Genet. Mol. Res. 4(4):
Birth of a healthy child by a woman with inherited Xq duplications who had experienced stillbirths
Authors: Y. Dong, S.C. Chen, X.W. Yu, E. Fadlalla, F. Jin and R.Z. Liu
23-year-old woman who had experienced repeated stillbirths, was found to carry an additional segment on the long arm of the X chromosome. Array comparative genomic hybridization (aCGH) confirmed the origin of the 2 duplications (about 17.11 Mb). Thus, her karyotype was 46, X, dup (X) (q13.2-q21.1), dup(X) (q21.32-q22.1). We de.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.17.9
- DOI:
- http://dx.doi.org/10.4238/2014.June.17.9
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: the experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH
Authors: C.B. Vendrame-Goloni, A.B. Carvalho-Salles, M.A. Ruiz,O. Ricci J�ºnior, M. Varella-Garcia4 and A.C. Fett-Conte
Investigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluoresce.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr396
- DOI:
- 10.4238/vol7-2gmr396
An improved reverse dot hybridization for simple and rapid detection of adefovir dipivoxil-resistant hepatitis B virus
Authors: Y. Hu, W.L. Zhang, S.L. Xie, Y. Zhao, J.L. Hu, X.F. Cai, G.Q. Lai and A.L. Huang
Early detection of adefovir dipivoxil-resistant mutants during long-term treatment of chronic hepatitis B virus (HBV) infection with this drug is of great clinical importance. We developed an improved reverse dot hybridization test for simple and rapid detection of the rtA181V/T and rtN236T mutations associated with adefovir d.. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.9.6
- DOI:
- http://dx.doi.org/10.4238/2012.January.9.6
Subtelomeric region of chromosome 2 in patients with autism spectrum disorders
Authors: A. Barbosa-Gon�§alves, C.B. Vendrame-Goloni, A.L.B. Martins and A.C. Fett-Conte
Autism spectrum disorders are severe psychiatric diseases commonly identified in the population. They are diagnosed during childhood and the etiology has been much debated due to their variations and complexity. Onset is early and characterized as communication and social interaction disorders and as repetitive and stereotyped.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr448
- DOI:
- 10.4238/vol7-2gmr448