Hybridization
Uses and limitations of two molecular cytogenetic techniques for the study of arrested embryos obtained through assisted reproduction technology
Authors: Maria C. Muhlmann, Alejandro O. Laudicina, Claudia Perandones, Maria V. Bertolino, Andrea Marazzi, Carlos J. Quintans, Monica Donaldson, Walter Bozzo and Sergio Pasqualini
We studied chromosomal abnormalities in arrested embryos produced by assisted reproductive technology with fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in order to determine the best technique for evaluating chromosomal aneusomies to be implemented in different situations. We examined i.. Read More»
- Genet. Mol. Res. 4(2):
Nucleolar behavior during meiosis in four species of phyllostomid bats (Chiroptera, Mammalia)
Authors: M.R. Beguelini, S.R.C. Marchesin, M.T.V. Azeredo-Oliveira and E. Morielle-Versute
We analyzed the behavior of the nucleolus, nucleolar structures and nucleolus organizer regions (NORs) during meiotic division in four species of phyllostomid bats that have different numbers and locations of NORs. Nucleoli began disassembly at leptotene, and the subcomponents released from the nucleolus were dispersed in the .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1060
- DOI:
- 10.4238/vol10-2gmr1060
Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages
Authors: M.Q. Zhang, C.A. Ma, W.W. Lv, Y.H. Huang, D.L. Wang and Y.L. Zhao
To better understand the reproductive transformation mechanism of Daphnia carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, and 85.11% identity to orthologous genes in .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.10
- DOI:
- 10.4238/2015.June.1.10
Identification and characterization of differentially expressed genes during incompatible interaction between the foliar rust Melampsora larici-populina and poplar
Authors: Z.J. Chen, Z.M. Cao and Z.D. Yu
Poplars are extensively cultivated worldwide, and their susceptibility to the foliar rust fungus leads to considerable damages in plantations. To better understand the molecular basis of poplar responses to the foliar rust, suppression subtractive hybridization was used to identify the potential important or novel genes involv.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.12
- DOI:
- 10.4238/2014.March.24.12
Chromosomal localization and partial sequencing of the 18S and 28S ribosomal genes from Bradysia hygida (Diptera: Sciaridae)
Authors: V.P. Gaspar, E.L.T. Shimauti and M.A. Fernandez
In insects, ribosomal genes are usually detected in sex chromosomes, but have also or only been detected in autosomal chromosomes in some cases. Previous results from our research group indicated that in Bradysia hygida, nucleolus organizer regions were associated with heterochromatic regions of the autosomal C chromosome, usi.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.26.6
- DOI:
- 10.4238/2014.March.26.6
Cytogenetic characterization of species of the family Heptapteridae (Teleostei: Siluriformes) from the Cuiab�¡ and Iva� River Basins, Brazil
Authors: L.M. Barbosa, G.E.B. Ferreira, A.C. Prizon, A.L.B. Portela-Castro and I.C. Martins-Santos
We cytogenetically characterized three species of Heptapteridae (Pimelodella sp, Pimelodella taenioptera, and Imparfinis schubarti) by investigating the distribution of constitutive heterochromatin and nucleolar organizer regions by silver nitrate impregnation (Ag-NOR) and fluorescence in situ hybridization. Pimelodella sp showed had a diploid number (2n).. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.12
- DOI:
- 10.4238/2015.July.31.12
Physical mapping of 18S rDNA and heterochromatin in species of family Lygaeidae (Hemiptera: Heteroptera)
Authors: V.B. Bardella, T.R. Sampaio, N.B. Venturelli, A.L. Dias,L. Giuliano-Caetano, J.A.M. Fernandes and R. da Rosa
Analyses conducted using repetitive DNAs have contributed to better understanding the chromosome structure and evolution of several species of insects. There are few data on the organization, localization, and evolutionary behavior of repetitive DNA in the family Lygaeidae, especially in Brazilian species. To elucidate the phy.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.26.7
- DOI:
- 10.4238/2014.March.26.7
Construction and analysis of a subtractive cDNA library of early embryonic development in duck
Authors: Y.L. Liu, L.X. Zhong, J.J. Li, J.D. Shen, D.Q. Wang, Z.R. Tao, F.X. Shi and L.Z. Lu
Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early develo.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.8.5
- DOI:
- 10.4238/2013.July.8.5
Mitotic and meiotic behavior of rye chromosomes in wheat - Psathyrostachys huashanica amphiploid x triticale progeny
Authors: Q. Xie, H. Kang, D.L. Sparkes, S. Tao, X.M. Fan, L. Xu, X. Fan, L. Sha, H. Zhang, Y. Wang, J. Zeng and Y. Zhou
The dynamics of rye chromosomes during mitosis and meiosis was analyzed in a subset comprising 33 F3 lines from the cross of wheat, Psathyrostachys huashanica amphiploid (AABBDDNsNs) and hexaploid triticale (AABBRR), as visualized by genomic in situ hybridization. The results indicated that 31 of the total lines contained 4-14 rye chromosomes. Twenty-eigh.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.16
- DOI:
- 10.4238/2013.January.4.16
Comparative genomic hybridization analysis of rice dwarf mutants induced by gamma irradiation
Authors: J.E. Hwang, S.-H. Kim, I.J. Jung, S.M. Han, J.-W. Ahn, S.-J. Kwon, S.H. Kim, S.-Y. Kang, D.S. Kim and J.-B. Kim
Comparative genomic hybridization (CGH) is a powerful tool used to analyze changes in copy number, polymorphisms, and structural variations in the genome. Gene copy number variation (CNV) is a common form of natural diversity in the genome, which can create new genes and alter gene structure. Thus, CNVs may influence phenotypi.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049092
- DOI:
- 10.4238/gmr15049092