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Hybridization

Human Genetics   Original Articles

Correlation of Y-chromosome multiple segmental deletions and chromosomal anomalies in non-obstructive azoospermic males from northeastern China

Authors: R.-L. Dai, R.-X. Wang, J.-L. Jin, G.-N. Niu, J.-Y. Lee, S.-B. Li and R.-Z. Liu

We investigated the frequency and types of Y-chromosome microdeletions and chromosomal anomalies in non-obstructive azoospermic and severely oligozoospermic infertile males in northeastern China. The sample consisted of 519 infertile males (456 azoospermic, 63 severely oligozoospermic). PCR assays for Y-chromosome microdeletions and chromosome analysis we.. Read More»

Genet. Mol. Res. 11(3):
2012.May.10.3
DOI:
10.4238/2012.May.10.3
Plant Genetics   Research Article

Natural hybridization between Gossypium mustelinum and exotic allotetraploid cotton species

Authors: I.P.P. de Menezes1, J.O. da Silva1, G. Malafaia1, R.D.D. Silveira1 andP.A.V. Barroso2

Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridiza.. Read More»

Genet. Mol. Res. 14(4):
2015.October.29.40
DOI:
10.4238/2015.October.29.40
Human Genetics   Research Article

`Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients

Authors: C.L. Wei1, J.L. Cheng, W.C. Yang, L.Y. Li, H.C. Cheng and J.J. Fu1

Y chromosomal microdeletions at the azoospermia factor locus and chromosome abnormalities have been implicated as the major causes of idiopathic male infertility. A marker chromosome is a structurally abnormal chromosome in which no part can be identified by cytogenetics. In this study, to identify the origin of the marker chromosomes and to perform a gen.. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.11
DOI:
10.4238/2015.November.18.11
Microbial Genetics   Research Article

Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9

Authors: X. Sun1, N.J. Xu1, L.Z. Jiang1, W. Hu1, M.H. Fan1 and Z.M. Liu2

A strain of the microalga Chlorella pyrenoidosa F-9 in our laboratory showed special characteristics when transferred from autotrophic to heterotrophic culture. In order to elucidate the possible metabolic mechanism, the gene expression profiles of the autonomous organelles in the green alga C. pyrenoidosa under autotrophic and heterotrophic cultivation w.. Read More»

Genet. Mol. Res. 13(4):
2014.October.20.17
DOI:
10.4238/2014.October.20.17
Microbial Genetics   Research Article

Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance

Authors: M. Lu*, L. Wang*, J. Zhang, S. Sun, Y. Li, W. Du, J. Wu, J. Zhao, Q. Yang and X. Chen

A wheat germplasm line 13-2-2 with resistance to powdery mildew was isolated; this line was derived from common wheat cv. W770B and rye, Secale cereale L. (2n = 2x = 14, RR). The line was characterized based on cytological, genomic in situ hybridization (GISH), sequence-characterized amplified region (SCAR), and simple sequence repeat (SSR) analyses. The .. Read More»

Genet. Mol. Res. 13(4):
2014.December.18.10
DOI:
10.4238/2014.December.18.10
Plant Genetics   Research Article

A rapid method to increase the number of F1 plants in pea (Pisum sativum) breeding programs

Authors: M.A. Esp�³sito P. Almir�³n I. Gatti V.P. Cravero F.S.L. Anido E.L. Cointry

In breeding programs, a large number of F2 individuals are required to perform the selection process properly, but often few such plants are available. In order to obtain more F2 seeds, it is necessary to multiply the F1 plants. We developed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of seeds using 6-benzylaminop.. Read More»

Genet. Mol. Res. 11(3):
2012.June.18.1
DOI:
10.4238/2012.June.18.1
Plant Genetics   Research Article

Selecting black-spot resistant papaya genotypes derived from backcrossing and hybrids

Authors: T.P.S. Poltronieri, S.F. Silveira, M. Vivas, R. Santa Catarina,D.F.M. Cortes, A.O.N. Azevedo and M.G. Pereira

Papaya crop is important to Brazilian agribusiness. However, the expansion of papaya cultivation in the country is affected by the absence of commercial cultivars presenting good disease resistance. The black-spot caused by the fungus Asperisporium caricae is the most damaging foliar disease affecting Brazilian papaya crops. T.. Read More»

Genet. Mol. Res. 16(1):
gmr16019401
DOI:
10.4238/gmr16019401
Human Genetics   Research Article

New insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes

Authors: K.C. Faria1, S.R.C. Marchesin3, P.R.L. Moreira2, M.R. Beguelini2and E. Morielle-Versute3

Molossidae species, Cynomops abrasus (2n = 34, fun­damental number, FN = 64), Eumops auripendulus (2n = 42, FN = 62), Molossus rufus (2n = 48, FN = 64), Molossops temminckii (2n = 48, FN = 64), and Nyctinomops laticaudatus (2n = 48, FN = 64), and Phyl­lostomidae species, Phyllostomus discolor (2n = 32, FN = 60), have karyotypes with different chro.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr637
DOI:
10.4238/vol8-3gmr637
Human Genetics   Research Article

Salt stress causes a shift in the localization pattern of germin gene expression

Authors: M. Caliskan

The response of plants to biotic and abiotic stress factors involves biochemical, physiological, morphological, and developmental changes. Salt stress has been the subject of extensive studies due to the low salt tolerance of many crop plants. Germin and germin-like gene products are known to be involved in various aspects of .. Read More»

Genet. Mol. Res. 8(4):
Microbial Genetics   Research Article

Molecular cytogenetic characterization of the Aegilops biuncialis karyotype

Authors: J. Wang W. Zhang H. Zhao F.R. Li Z.G. Wang J. Ji X.Q. Zhang D.W. Wang J.M. Li

Aegilops biuncialis can be hybridized with wheat (Triticum spp) and has been used for wheat breeding and genetic studies. The A. biuncialis karyotype (Ub Ub Mb Mb) was investigated based on three A. biuncialis accessions grown in China. Two pairs of SAT chromosomes were identified as 1Ub and 5Ub, with a karyotype formula of 2n = 4x = 28 = 14m + 10sm + 4st. F.. Read More»

Genet. Mol. Res. 12(1):
2013.March.11.16
DOI:
10.4238/2013.March.11.16