Hybridization
Optimal clone identifier for genomic shotgun libraries: â??OC Identifier toolâ?
Authors: Cantao ME, Ferreira JE and Lemos EGM
In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of puttin.. Read More»
- Genet. Mol. Res. 6(4):
Expression of the ROR?± gene in Inner Mongolian cashmere goat hair follicles
Authors: Y.H. Zhao, Z.H. Liu, L. Wang, H.M. Xiao, C.G. Du, Y.J. Zhang,R. Su and J.Q. Li
The expression of retinoid-acid-related orphan receptor ?± (ROR?±) was evaluated at the mRNA level using real-time polymerase chain reaction (qRT-PCR), and its expression localization was determined by in situ hybridization of adult Inner Mongolian cashmere goats at different times of the year. In situ hybridization demonstrated that ROR?±was expressed.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.11
- DOI:
- 10.4238/2015.January.23.11
Differential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization
Authors: A. Morielle-Souza, M.T.V. Azeredo-Oliveira
A comparative study of holocentric chromosomes in the triatomine species Panstrongylus megistus, Rhodnius pallescens and Triatoma infestans was carried out in order to characterize heterochromatin, rDNA active sites and nucleolar proteins. Cytological preparations of seminiferous tubules were stained by silver impregnation, C .. Read More»
- Genet. Mol. Res. 6(3):
Localization of HSP single-copy genes by inexpensive, permanent non-fluorescent in situ hybridization on meiotic chromosomes of the grasshopper Schistocerca pallens (Acrididae)
Authors: T.T. Rieger, S.V. Oliveira-Silva, I.A. Pach?ªco, B.S. Chagas, J.F. Santos
There have been many studies on Schistocerca gregaria and Locusta migratoria, which are important grasshopper pests in many parts of the world. However, the main pest grasshopper species in Brazil, S. pallens, Rhammatocerus schistocercoides and Stiphra robusta, are very poorly characterized genetically. We adapted a permanent .. Read More»
- Genet. Mol. Res. 6(3):
Complete asynapsis resulting in 2n pollen formation in Paspalum jesuiticum Parodi (Poaceae)
Authors: R.A. Bernardo Filho, A.C.C. Santos, F.H.D. Souza, J.F.M. Valls and M.S. Pagliarini
Cytological investigation revealed complete asynapsis during microsporogenesis in 2 wild accessions of Paspalum jesuiticum collected in distinct Brazilian regions. Both accessions were hexaploid (2n = 6x = 60) and 60 univalents could be counted at diakinesis. In this phase, the majority of meiocytes exhibited univalents with b.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.17.9
- DOI:
- 10.4238/2014.January.17.9
Identification and expression analysis of the Broad-Complex core protein isoform 6 (BR-C Z6) gene in the giant tiger shrimp Penaeus monodon (Penaeidae: Decapoda)
Authors: A. Buaklin, S. Klinbunga and P. Mensveta
Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440.. Read More»
- Genet. Mol. Res. 10(4):
Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean
Authors: J. Sun, J. Li, M. Liu, B.B. Zhang, D.M. Li, M. Wang, C. Zhang,W.B. Li, A.Y. Su and X.X. Wu
The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA librar.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.20
- DOI:
- 10.4238/2015.January.30.20
Identification of PML/RAR?± fusion gene transcripts that showed no t(15;17) with conventional karyotyping and fluorescent in situ hybridization
Authors: A. Choughule S. Polampalli P. Amre S. Shinde S. Banavali K. Prabhash R. Nair P.G. Subramanian S. Gujral P.M. Parikh
Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17)(q22;q11-21), resulting in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARα) genes. Using conventional cytogenetic methods, these translocations are normally detected in about 70-90% of patients.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr488
- DOI:
- 10.4238/vol8-1gmr488
Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization
Authors: J.H. He F.W. Ma Y.Y. Chen H.R. Shu
Embryo abortion in mango severely damages mango production worldwide. The mechanisms by which the mango embryos abort have long been an intriguing question. We used subtractive suppression hybridization to investigate the differentially expressed genes involved in this process. We generated 2 cDNA libraries from normal seed and aborted seed embryos of mango .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.14.4
- DOI:
- 10.4238/2012.November.14.4
Identification and analysis of the jnk1 gene in polyploid hybrids of red crucian carp (Carassius auratus red var.) and common carp (Cyprinus carpio L.)
Authors: Y.M. Xiao, M.G. Jiang, Z.W. Luo, Y.H. Zhou, S. Wen, M. Wang,C. Zhang and S.J. Liu
c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase superfamily. The allotetraploid crucian carp is a product of distant hybridization of female red crucian carp with male common carp. It is the first natural case of an allotetraploid with stable genetic characters, including fertility .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.1
- DOI:
- 10.4238/2014.February.19.1