Hybridization
Molecular characterization of the gene checkpoint homolog 1 in Daphnia carinata during different reproductive phases
Authors: L. Kong, H.X. Li, D.L. Wu, G.R. Xu, D.L. Wang and Y.L. Zhao
Full-length cDNA of the gene checkpoint homolog 1 (Chk1) was cloned from Daphnia carinata and designated DcarChk1. DcarChk1 cDNA was 1817 bp in length and encoded a 497-amino acid polypeptide. Phylogenetic analyses revealed that DcarChk1 was most closely related to Chk1 of Daphnia pulex, followed by homologous genes of insects.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026132
- DOI:
- 10.4238/gmr.15026132
Cross-species amplification and polymorphism of microsatellite loci in Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) in Brazilian cropping systems
Authors: N.A. Leite, A.S. Corr�ªa, A. Alves-Pereira, J.B. Campos, M.I. Zucchi and C. Omoto
The Old World bollworm Helicoverpa armigera (Hübner) was recently discovered in Brazil. This species is closely related to the New World bollworm H. zea (Boddie), and mating between these species has already been reported under laboratory conditions. Here, we tested the cross-species amplification of 20 microsatellite (SS.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027890
- DOI:
- 10.4238/gmr.15027890
Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus
Authors: L. Zhu,C.H. Li, X.R. Su, C.Y. Guo, Z. Wang, C.H. Jin,Y. Li,T.W. Li
Rapid and efficient growth is a major consideration and challenge for global mariculture. The differential growth rate of the sea cucumber, Apostichopus japonicus, has significantly hampered the total production of the industry. In the present study, forward and reverse suppression subtractive hybridization libraries were constructed and sequenced from a .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.20.4
- DOI:
- 10.4238/2013.August.20.4
Prenatal diagnosis of a partial trisomy 13q (q14�qter): phenotype, cytogenetics and molecular characterization by spectral karyotyping and array comparative genomic hybridization
Authors: I.N. Machado, J.K. Heinrich, C. Campanhol, R.M. Rodrigues-Peres, F.M. Oliveira and R. Barini
Partial trisomy 13q is an uncommon chromosomal abnormality with variable phenotypic expression. We report prenatal diagnosis of partial trisomy 13q in a fetus with partial agenesis of the cerebellar vermis, partial agenesis of the corpus callosum, hydrops and polyhydramnios. G-banding karyotyping, spectral karyotyping and arra.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr716
- DOI:
- 10.4238/vol9-1gmr716
Genetic, embryonic and anatomical study of an interspecific cassava hybrid
Authors: N.M.A. Nassar, D.Y. Hashimoto and D.G. Ribeiro
A molecular, anatomical and cytogenetic study of an interspecific hybrid between Manihot esculenta (cassava) and the wild species M. oligantha was carried out. Cytogenetics revealed relatively complete chromosome pairing and high viability of the pollen grains. Ovule structure examined by the clearing method showed polyembryon.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr746
- DOI:
- 10.4238/vol9-1gmr746
Assessment of clinical scoring systems for the diagnosis of Williams-Beuren syndrome
Authors: D.E.S. Leme, D.H. Souza, G. Mercado, E. Pastene, A. Dias and D. Moretti-Ferreira
Williams-Beuren syndrome (WBS) is a genetic disorder characterized by physical and intellectual developmental delay, associated with congenital heart disease and facial dysmorphism. WBS is caused by a microdeletion on chromosome 7 (7q11.23), which encompasses the elastin (ELN) gene and about 27 other genes. The gold standard for WBS laboratory diagnosis i.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.7
- DOI:
- 10.4238/2013.September.4.7
Apomixis in cassava: advances and challenges
Authors: D.Y.H. Freitas and N.M.A. Nassar
Cassava is the most important staple crop in the Tropics and Subtropics. Apomixis may revolutionize its production due to various attributes. These potential advantages include production by true seed, maintaining cultivar superiority over generations without segregation, and avoiding contamination by bacteria and viruses. Historically, apomixis was initi.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.14
- DOI:
- 10.4238/2013.April.2.14
Smallest critical region for microcephaly in a patient with mosaic ring chromosome 13
Authors: P.-H. Su, C.-P. Chen, Y.-N. Su, S.-J. Chen, L.-L. Lin and J.-Y. Chen
A ring chromosome 13 or r(13) exhibits breakage and reunion at breakage points on the long and short arms of chromosome 13, with deletions of the chromosomal segments distal to the breakage points. The r(13) chromosome accounts for approximately 20% of ring chromosomes compatible with life. We describe a female patient with mental retardation, growth reta.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.2
- DOI:
- 10.4238/2013.April.25.2
Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip
Authors: M.Z. Zhang, X.F. Zhang, X.M. Chen, X. Chen, S. Wu and L.L. Xu
The enzyme-linked probe hybridization chip utilizes a method based on ligase-hybridizing probe chip technology, with the principle of using thio-primers for protection against enzyme digestion, and using lambda DNA exonuclease to cut multiple PCR products obtained from the sample being tested into single-strand chains for hybridization. The 5'-end amino-l.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.10
- DOI:
- 10.4238/2015.August.10.10
Confirmation of cross-fertilization using molecular markers in ornamental passion flower hybrids
Authors: L.D.H.C.S. Concei�§�£o, G.O. Belo, M.M. Souza, S.F. Santos, C.B.M. Cerqueira-Silva and R.X. Corr�ªa
Several interspecific Passiflora hybrids are produced in the northern hemisphere for the ornamental plant market. In Brazil, production of passion flower hybrids is limited to the introgression of genes into the main cultivated species, yellow passion fruit, to be used as rootstocks. Confirmation of hybridization in the initia.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr894
- DOI:
- 10.4238/vol10-1gmr894