Animal Genetics   Research Article

Comparing KISS1 and GPR54 genes between the two breeds of goats with low and high prolificacy

Authors: Jie Zheng, Sayed Haidar Abbas Raza, Xiang-Dong Zi, Jian-Yuan Lu

There is increasing evidence that kisspeptin, the peptide product of KISS1 gene and its receptor (GPR54), regulate reproductive processes through actions on other organs than hypothalamus. Therefore, the study for the first time, was conducted to investigate the nucleotide sequences and mRNA expression levels of KISS1 and GPR54 genes in pituitary, endomet.. Read More»

Genet. Mol. Res. 17(4):
http://dx.doi.org/gmr16039922
DOI:
http://dx.doi.org/10.4238/gmr16039922
Human Genetics   Research Article

Sub-lethal doses of neonicotinoid and carbamate insecticides reduce the lifespan and alter the expression of immune health and detoxification related genes of honey bees (Apis mellifera)

Authors: Hassan Tarek, Mollah Md. Hamiduzzaman, Nuria Morfin and Ernesto Guzman-Novoa

Honey bees (Apis mellifera) are exposed to sublethal doses of insecticides, but little is known about insecticide effects on their survivorship associated to health-related gene expression. To test the effect of sublethal doses of clothianidin, imidacloprid and carbaryl on the lifespan and health of honey bees, workers were or.. Read More»

Genet. Mol. Res. 17(2):
gmr16039908
DOI:
10.4238/gmr16039908
Animal Genetics   Research Article

Expression of lipid metabolism and myosin heavy chain genes in pigs is affected by genotype and dietary lysine

Authors: RC Veloso, PS Lopes, MS Duarte, FF Silva, A Saraiva, ML Chizzotti, W Silva and SEF Guimarães

Expression of lipid metabolism and myosin heavy chain genes was evaluated in skeletal muscle of pig’s genotypes submitted to different dietary digestible lysine contents. Fifty-two barrows and fifty gilts from three genotypes (G: Piau, Duroc and Pietrain crossbreds) were assigned to three dietary digestible lysine conten.. Read More»

Genet. Mol. Res. 17(2):
gmr16039904
DOI:
10.4238/gmr16039904
Human Genetics   Research Article

Screening and identification of candidate genes essential for cervical lymph node metastasis in nasopharyngeal carcinoma with microarrays

Authors: Ming Fu, Yudong Ye, Chi Zhang, Zhuo Chen, Zhenchao Zhu, Yang Peng, Xiangning Zhang, Qianhui Qiu

The aim of the present study is to identify genes that are crucial for the cervical lymph node metastasis, which predicts poor prognosis of nasopharyngeal carcinoma (NPC), endemic in southern China. Microarrays with probes to determine genomic expression profile and promoter methylation status in tumor specimens were used, to .. Read More»

Genet. Mol. Res. 17(1):
gmr16039892
DOI:
10.4238/gmr16039892
Animal Genetics   Research Article

Analysis of melanopsin gene expression in the rabbit retina at different ages

Authors: M.M. Lira-Carrera, B.E. Gutiérrez-Amavizca, L.J. Álvarez-Araujo, M. Aguirre-Ramírez and J.A. Pérez-León

Melanopsin is the photopigment of intrinsically photosensitive retinal ganglion cells that mediate non-visual responses to light. The aim of this study was to describe and analyze melanopsin gene expression in the rabbit retina at different ages and compare its expression with the prototypic gene of retinal ganglion cells (Thy.. Read More»

Genet. Mol. Res. 16(3):
gmr16039767
DOI:
10.4238/gmr16039767
Animal Genetics   Research Article

Cadmium accumulation and metallothionein gene expression in the liver of swamp eel (Monopterus albus) collected from the Mae Sot District, Tak Province, Thailand

Authors: M. Wahid, S. Prasarnpun and N. Yimtragool

Cadmium (Cd) is produced mainly as a by-product of zinc mining. In Thailand, the largest zinc mine is located in the Mae Sot district, Tak Province. Samples of Monopterus albus were collected from paddy fields in 4 sites, three downstream and one upstream from the zinc mine. The upstream site was considered to be uncontaminate.. Read More»

Genet. Mol. Res. 16(3):
gmr16039748
DOI:
10.4238/gmr16039748
Plant Genetics   Research Article

Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures

Authors: P.A. Auler, L.C. Benitez, M.N. do Amaral, I.L. Vighi, G.S. Rodrigues, L.C. da Maia and E.J.B. Braga

Rice is a cereal that presents a great ability to adapt to different soil and climate conditions. However, as it is a tropical crop with C3 metabolism, it performs better in warm temperatures with high solar radiation. Tolerance to stress caused by low temperatures is a highly complex process that involves various metabolic pa.. Read More»

Genet. Mol. Res. 16(2):
gmr16029695
DOI:
10.4238/gmr16029695
Animal Genetics   Research Article

Differential gene expression in the peripheral blood of Chinese Sanhe cattle exposed to severe cold stress

Authors: Q. Xu, Y.C. Wang, R. Liu, L.F. Brito, L. Kang, Y. Yu, D.S. Wang, H.J. Wu and A. Liu

Livestock is an important food resource for the inhabitants of cold regions, such as northern Asia and alpine regions, where agriculture is limited. In these regions, cold stress largely affects livestock production, thereby reducing the productivity and survival of animals. Despite the importance of breeding cold-tolerant ani.. Read More»

Genet. Mol. Res. 16(2):
gmr16029593
DOI:
10.4238/gmr16029593
Plant Genetics   Research Article

De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress

Authors: A.A. Belesini, F.M.S. Carvalho, B.R. Telles, G.M. de Castro, P.F. Giachetto, J.S. Vantini, S.D. Carlin, J.O. Cazetta, D.G. Pinheiro and M.I.T. Ferro

Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated.. Read More»

Genet. Mol. Res. 16(2):
gmr16028845
DOI:
10.4238/gmr16028845
Plant Genetics   Research Article

Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements

Authors: N. Marini, C.B. Bevilacqua, M.V. Büttow, M.C.B. Raseira and S. Bonow

Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Pru.. Read More»

Genet. Mol. Res. 16(2):
gmr16029666
DOI:
10.4238/gmr16029666