Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers
Authors: I.G. Almeida, P. Ianella, M.T. Faria, S.R. Paiva and A.R. Caetano
Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.17
- DOI:
- 10.4238/2013.December.4.17
Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation
Authors: X. Wang1, H. Wang1, N. Jiang1, W. Lu1, X.F. Zhang1 and J.Y. Fang2
Pancreatic adenocarcinoma is a lethal disease because it is inoperable at the time of diagnosis. Therefore, the search for new therapeutic approaches is critical. The abnormal expression of the mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK) pathway and alteration in epigenetic modification (DNA methylation and acetylation of .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.6
- DOI:
- 10.4238/2013.November.18.6
Expression of cationic amino acid transporters, carcass traits, and performance of growing pigs fed low-protein amino acid-supplemented versus high protein diets
Authors: A. Morales, F. Grageola, H. GarcÃ?Âa, A. Araiza, R.T. Zijlstra and M. Cervantes
Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.9
- DOI:
- 10.4238/2013.October.18.9
Identification of the isoamylase 3 gene in common wheat and its expression profile during the grain-filling period
Authors: G.Z. Kang, G.Q. Liu, W. Xu, Y.J. Zhu, C.Y. Wang, H.Q. Ling and T.C. Guo
In higher plants, isoamylase-type starch debranching enzyme catalyzes the α-1,6-glucosidic linkages of glycogen and phytoglycogen. We cloned an isoamylase-type starch debranching enzyme ISA3 cDNA sequence (2883 bp), designated as TaISA3, from common wheat (Triticum aestivum), using the rapid amplification of cDNA ends method. The open reading frame .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.17
- DOI:
- 10.4238/2013.February.28.17
Associations between clinical characteristics and oncogene expression in patients with non-small cell lung cancer
Authors: Y. Han, D.P. Yu, S.J. Zhou, X.Y. Song, Y.S. Li, N. Xiao, Z.D. Liu, X.J. Sun, Q.Y. Zhao and S.K. Liu.
More than 40 oncogenes associated with non-small cell lung cancer (NSCLC) have been identified with varied gene expression. The correlations between specific clinical characteristics and oncogene expression in NSCLC patients were examined. From October 2011 to September 2012, a total of 60 patients with NSCLC (male:female, 34:24; mean age, 59.5 ± 1.. Read More»
- Genet. Mol. Res. 13(4):
Lack of genotoxicity in Astyanax bimaculatus and Oreochromis niloticus of 17�±-methyltestosterone used in fish hatcheries to produce male monosex populations
Authors: C.L.G. Rivero-Wendt1, A.L. Miranda-Vilela1, M.F.N. Ferreira1, F.S. Amorim2, V.A.G. da Silva2, H. Louvandini3 and C.K. Grisolia1
17α-Methyltestosterone (MT) is widely used in fish hatcheries of many countries to produce male monosex populations. Its genotoxic risk to fish species is not well known and studies in other in vivo models are still inconclusive. MT was tested for genotoxicity in the fish species Oreochromis niloticus (tilapia), a target species, and Astyanax bimacu.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.24.13
- DOI:
- 10.4238/2013.October.24.13
Comparative sodium dodecylsulfate-polyacrylamide gel electrophoresis and restricted fragment length polymorphism among fenugreek accessions
Authors: E.A. Haliem and A.A. Al-Huqail
Protein and DNA polymorphismswere surveyed among seven accessions of wild fenugreek (Trigonellafoenum-graecum L.) to estimate their genetic diversity and relationships. Samples were obtained from diverse ecogeographical areas in Saudi Arabia and Yemen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of seed storage protein sh.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.16
- DOI:
- 10.4238/2013.December.4.16
Registries of cleft lip/palate cases between 2004 and 2010 in Denizli, Turkey
Authors: A.G. Tomat�±r, I. Acikbas, B. Akdag and A. K�¶ksal
Oral clefts are one of the most common birth defects in humans. However, few population-based studies of these defects have been carried out in Turkey. Our objective was to determine the registries of cases of cleft lip and palate. All cases of cleft lip and palate referred to central state hospitals in Denizli between January 2000 and May 2010 were inves.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.19.4
- DOI:
- 10.4238/2013.February.19.4
Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense
Authors: Y.P. Zhang, H. Qiao, W.Y. Zhang, S.M. Sun, S.F. Jiang, Y.S. Gong, Y.W. Xiong, S.B. Jin and H.T. Fu
In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs. Real-ti.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.8
- DOI:
- 10.4238/2013.October.18.8
ReSeqTools: an integrated toolkit for large-scale next-generation sequencing based resequencing analysis
Authors: W. He, S. Zhao, X. Liu, S. Dong, J. Lv, D. Liu, J. Wang and Z. Meng
Large-scale next-generation sequencing (NGS)-based resequencing detects sequence variations, constructs evolutionary histories, and identifies phenotype-related genotypes. However, NGS-based resequencing studies generate extraordinarily large amounts of data, making computations difficult. Effective use and analysis of these data for NGS-based resequencin.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.15
- DOI:
- 10.4238/2013.December.4.15