Using PCR-RFLP for sexing of the endangered Gal�¡pagos petrel (Pterodroma phaeopygia)
Authors: L. PatiÃ?±o, M. Cruz, P. MartÃ?Ânez and V. CedeÃ?±o-Escobar
Pterodroma phaeopygia is a critically endangered avian species of the Galápagos Islands. This bird is sexually monomorphic, making it difficult to identify the sex. This information, however, is relevant to studies of behavior, ecology, and management of wild or captive populations. Here, we aimed to implement a molecular approach for determining s.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.13
- DOI:
- 10.4238/2013.October.18.13
TaALMT1 promoter sequence compositions, acid tolerance, and Al tolerance in wheat cultivars and landraces from Sichuan in China
Authors: C. Han1*, S.F. Dai1*, D.C. Liu1, Z.J. Pu2, Y.M. Wei1, Y.L. Zheng1, D.J. Wen1, L. Zhao1 and Z.H. Yan1
Previous genetic studies on wheat from various sources have indicated that aluminum (Al) tolerance may have originated independently in USA, Brazil, and China. Here, TaALMT1 promoter sequences of 92 landraces and cultivars from Sichuan, China, were sequenced. Five promoter types (I', II, III, IV, and V) were observed in 39 cultivars, and only three promot.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.9
- DOI:
- 10.4238/2013.November.18.9
In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells
Authors: M.A. Ali, M. Abul Farah, F.M. Al-Hemaid and F.M. Abou-Tarboush
This study investigated the in vitro anticancer activities of a total of 14 wild angiosperms collected in Saudi Arabia. The cytotoxic activity of each extract was assessed against human breast adenocarcinoma (MCF-7) cell lines by using the MTT assay. Among the plants screened, the potential cytotoxic activity exhibited by the .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.9
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.9
Protein-protein interaction network and significant gene analysis of osteoporosis
Authors: X.M. Wu, X. Ma, C. Tang, K.N. Xie, J. Liu, W. Guo, Y.L. Yan, G.H. Shen and E.P. Luo
This study used DNA microarray data to identify differentially expressed genes of osteoporosis and provide useful information for treatments of the disease. We downloaded gene expression data of Osteoporosis GSE35956 from the Gene Expression Omnibus database, which included five normal and five osteoporosis samples. We then identified the differentially e.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.12
- DOI:
- 10.4238/2013.October.18.12
Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro
Authors: J. Kumchai1, J.-Z. Huang2, C.-Y. Lee2, F.-C. Chen2 and S.-W. Chin2
In vitro grown cabbage (Brassica oleracea var. capitata) seedlings exposed to excess molybdenum (Mo) ions exhibited severely reduced plant growth at the cotyledonary stage. Adding 80 mM proline (Pro) to the Mo-treated medium could help 50% seedlings to overcome the toxicity and grow true leaves. Under excess Mo stress, seedlings accumulated blue/purple an.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.8
- DOI:
- 10.4238/2013.November.18.8
Establishment and molecular characterization of a sweet potato germplasm bank of the highlands of Paran�¡ State, Brazil
Authors: L.K.P. Camargo1,2, A.F. M�³gor2, J.T.V. Resende1 and P.R. Da-Silva3
The sweet potato (Ipomoea batatas L.) is a crop of great importance in developing countries, as a food staple, for animal feed, and potentially for biofuel. Development of cultivars adapted to specific regions within these countries would be useful. To start a breeding program, the first step is the establishment of a germplasm bank. We initiated a sweet .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.7
- DOI:
- 10.4238/2013.November.18.7
Roles of Fas/Fasl, Bcl-2/Bax, and Caspase-8 in rat nonalcoholic fatty liver disease pathogenesis
Authors: C.P. Li, J.H. Li, S.Y. He, P. Li and X.L. Zhong
The aim of this study was to investigate the roles of Fas/FasL, Bcl-2/Bax, and Caspase-8 mRNA expressions in nonalcoholic fatty liver disease (NAFLD). The apoptosis percentage was measured by flow cytometry, the immunohistochemical assay was performed for the determination of Fas, FasL, Bcl-2, and Bax expressions, and a real-t.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.10
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.10
Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)
Authors: Y.L. Hou, X. Ding, W. Hou, B. Song, T. Wang, F. Wang, J. Li, J. Zhong, T. Xu, B.X. Ma, H.Q. Zhu, J.H. Li and J.C. Zhong
The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain rea.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.11
- DOI:
- 10.4238/2013.October.18.11
Genetic differences between the wild and hatchery-produced populations of Korean short barbeled grunter (Hapalogenys nitens) determined with microsatellite markers
Authors: H.S. An, H.W. Kang, H.S. Han, J.Y. Park, C.G. Hong, J. Park, J.I. Myeong and C.M. An
Short barbeled grunter, Hapalogenys nitens, is an economically important fishery resource. In Korea, this fish is in the early stage of domestication, and it has been regarded as the candidate marine fish species for prospective aquaculture diversification. This study presents a preliminary investigation of the future viability of sustainable fry producti.. Read More»
- Genet. Mol. Res. 13(4):
Development and characterization of new microsatellites for walnut (Juglans regia)
Authors: Z.Y. Zhang, J.W. Han, Q. Jin, Y. Wang, X.M. Pang and Y.Y. Li
The expressed sequence tag (EST) database represents a potentially valuable resource for the development of simple sequence repeat (SSR) markers for use in evolutionary studies. EST-SSRs reveal polymorphisms not only within the source taxon, but in related taxa as well. In this paper, we describe a case study in which the publicly available walnut (Juglan.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.10
- DOI:
- 10.4238/2013.October.18.10