Male infertility in Northeast China: molecular detection of Y chromosome microdeletions in azoospermic patients with Klinefelter�s syndrome
Authors: H.-G. Zhang, Z.-B. Zhang, R.-X. Wang, Y. Yu, X.-W. Yu, E. Fadlalla and R.-Z. Liu
The prevalence of microdeletions of azoospermia factor (AZF) among azoospermic Klinefelter’s syndrome (KFS) patients shows conflicting data. We aimed to detect this frequency in a Northeast Chinese population, and to investigate the possible association between AZF microdeletions and KFS by comparison with previous conflicting reports. Eighty men af.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.24.9
- DOI:
- 10.4238/2013.October.24.9
Effect of RNAi-mediated silencing of Livin gene on biological properties of colon cancer cell line LoVo
Authors: A.M. Zou, H.F. Wang, W.F. Zhu, F.X. Wang and J.J. Shen
This study aimed to investigate the effect of RNAi-mediated silencing of the Livin gene on biological properties of the colon cancer cell line LoVo. Interference vectors pSilencer4.1-Ll and pSilencer4.1-L2 targeting the Livin gene were constructed and transfected into LoVo cells. The expression of the Livin gene was determined.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.8
A multi-farm assessment of Greek black pig genetic diversity using microsatellite molecular markers
Authors: S. Michailidou, A. Kalivas, I. Ganopoulos, E. Stea, G. Michailidis, A. Tsaftaris and A. Argiriou
Local breeds are important for the maintenance of genetic diversity and future food security. Nowadays, the worldwide distribution of pigs is dominated by a few breeds, tending towards a severe loss of pig biodiversity. Thus, it is critical to maintain distinct populations of pig breeds. The Greek black pig, a breed raised loc.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.4
- DOI:
- 10.4238/2014.April.14.4
In vivo mechanism study of NGAL in rat renal ischemia-reperfusion injury
Authors: X.J. Zang1, S.X. An2, Z. Feng1, Y.P. Xia1, Y. Song and Q. Yu
This study aimed to determine the protective effect and mechanism of neutrophil gelatinase-associated lipocalin (NGAL) in rat kidney on ischemia/reperfusion injury (I/R). The rat I/R model was set up by cutting one kidney and clamping the contralateral renal pedicle for 45 min. Male SD rats were randomly divided into sham-oper.. Read More»
- Genet. Mol. Res. 13(4):
Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes
Authors: X.J. Rong, Y.J. Xu, Q.Y. Wang, M.J. Liao, X.Z. Liu, C.Y. Pan,Z. Zhang, Y.G. Wang
Coilia ectenes (Jordan and Seale 1905) is an important anadromous species that is an important resource at risk of extinction because of over-fishing, pollution, and coastal construction. To evaluate the genetic diversity of C. ectenes for use in breeding programs, elite microsatellite-enriched libraries were constructed and n.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.11
- DOI:
- 10.4238/2013.November.26.11
Isolation and characterization of microsatellites for the yam Dioscorea cayenensis (Dioscoreaceae) and cross-amplification in D. rotundata
Authors: L.R.G. Silva, M.M. Bajay, M. Monteiro, T.F. Mezette, W.F. Nascimento, M.I. Zucchi, J.B. Pinheiro and E.A. Veasey
Dioscorea cayenensis and Dioscorea rotundata are among the most important yam species for the humid and sub-humid tropics. We isolated nine polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. The nine primer pairs were validated in 22 D. cayenensis accessions, and were tested for trans.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.5
- DOI:
- 10.4238/2014.April.14.5
Multidrug-resistant genes of aminoglycoside-modifying enzymes and 16S rRNA methylases in Acinetobacter baumannii strains
Authors: J.-T. Wen, Y. Zhou, L. Yang and Y. Xu
We examined the distribution of genes of aminoglycoside-modifying enzymes and 16S rRNA methylases in multidrug-resistant Acinetobacter baumannii to explore the association of these genes with drug resistance. Strains isolated from clinical specimens were screened using an automatic microbial identification system, and 9 aminog.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.9
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.9
Molecular cloning and expression of the porcine S14R gene in Escherichia coli
Authors: Y.J. Guo1*, G.Z. Liu1*, C.M. Wang1, Y.Y. Wang2, H.J. Li2, K. Zhong2, W.F. Lu2, Y.L. Wang2, G.Y. Yang2
We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragm.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.6
- DOI:
- 10.4238/2013.October.10.6
Association of SIRT2 gene polymorphisms with body measurement and growth traits of Qinchuan cattle
Authors: L.S. Gui, W.C. Yang, C.P. Zhao, S.J. Wei, Z.D. Zhao and L.S. Zan
Silent information regulator 2 (SIRT2), a member of the Sirtuin family of class III nicotinamide adenine dinucleotide-dependent protein deacetylases, plays an important role in senescence, metabolism, and apoptosis. This study was conducted to detect potential polymorphisms of the bovine SIRT2 gene and explore their relationships with meat quality and bod.. Read More»
- Genet. Mol. Res. 13(4):
Developmental changes in IGF-I and MyoG gene expression and their association with meat traits in sheep
Authors: W. Sun, R. Su1, D. Li, H.H. Musa, Y. Kong, J.T. Ding, Y.H. Ma, L. Chen, Y.F. Zhang and W.Z. Wu
In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.6
- DOI:
- 10.4238/2014.April.14.6