Biplot analysis of strawberry genotypes recommended for the State of EspÃ?Ârito Santo
Authors: A.F. Costa, P.E. Teodoro, L.L. Bhering, N.R. Leal, F.D. Tardin and R.F. Daher
Most strawberry genotypes grown commercially in Brazil originate from breeding programs in the United States, and are therefore not adapted to the various soil and climatic conditions found in Brazil. Thus, quantifying the magnitude of genotype x environment (GE) interactions serves as a primary means for increasing average Brazilian strawberry yields, an.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038919
- DOI:
- 10.4238/gmr.15038919
Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers
Authors: P.V. Tiago, M.P. Carneiro-Le�£o, M.L.A. Lima, N.T. Oliveira and E.A. Luna-Alves Lima
Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará d.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1069
- DOI:
- 10.4238/vol10-3gmr1069
A picture of gene sampling/expression in model organisms using ESTs and KOG proteins
Authors: MaurÃ?Âcio de Alvarenga Mudado and JosÃ?© Miguel Ortega
The expressed sequence tag (EST) is an instrument of gene discovery. When available in large numbers, ESTs may be used to estimate gene expression. We analyzed gene expression by EST sampling, using the KOG database, which includes 24,154 proteins from Arabidopsis thaliana (Ath), 17,101 from Caenorhabditis elegans (Cel), 10,51.. Read More»
- Genet. Mol. Res. 5(1):
cDNA, genomic sequence cloning and overexpression of ribosomal protein gene L9 (rpL9) of the giant panda (Ailuropoda melanoleuca)
Authors: W.R. Hou, Y.L. Hou, G.F. Wu, Y. Song, X.L. Su, B. Sun and J. Li
The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mam.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1159
- DOI:
- 10.4238/vol10-3gmr1159
An empirical examination of the standard errors of maximum likelihood phylogenetic parameters under the molecular clock via bootstrapping
Authors: Carlos G. Schrago
The molecular clock theory has greatly enlightened our understanding of macroevolutionary events. Maximum likelihood (ML) estimation of divergence times involves the adoption of fixed calibration points, and the confidence intervals associated with the estimates are generally very narrow. The credibility intervals are inferred.. Read More»
- Genet. Mol. Res. 5(1):
Identification of genes encoding hypothetical proteins in open-reading frame expressed sequence tags from mammalian stages of Trypanosoma cruzi.
Authors: C. Martins, J.L. Reis-Cunha, M.N. Silva, E.G. Pereira, G.J. Pappas Jr., D.C. Bartholomeu and B. Zingales
Approximately 50% of the predicted protein-coding genes of the Trypanosoma cruzi CL Brener strain are annotated as hypothetical or conserved hypothetical proteins. To further characterize these genes, we generated 1161 open-reading frame expressed sequence tags (ORESTES) from the mammalian stages of the VL10 human strain. Sequ.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1140
- DOI:
- 10.4238/vol10-3gmr1140
In silico prediction of yeast deletion phenotypes
Authors: Soma Saha and Steffen Heber
Analysis of gene deletions is a fundamental approach for investigating gene function. We evaluated an algorithm that uses classification techniques to predict the phenotypic effects of gene deletions in yeast. We used a modified simulated annealing algorithm for feature selection and weighting. The selected features with high .. Read More»
- Genet. Mol. Res. 5(1):
Establishment of a heterologous system for the expression of Canavalia brasiliensis lectin: a model for the study of protein splicing
Authors: Walderly Melga�§o Bezerra, Cristina Paiva da Silveira Carvalho, Renato de Azevedo Moreira and Thalles Barbosa Grangeiro
During its biosynthesis in developing Canavalia brasiliensis seeds, the lectin ConBr undergoes a form of protein splicing in which the order of the N- and C-domains of the protein is reversed. To investigate whether these events can occur in other eukaryotic organisms, an expression system based on Pichia pastoris cells was es.. Read More»
- Genet. Mol. Res. 5(1):
DBCollHIV: a database system for collaborative HIV analysis in Brazil
Authors: Luciano V. Ara�ºjo, Marcelo A. Soares, Suelene M. Oliveira, Pedro Chequer, Amilcar Tanuri, Ester C. Sabino and Jo�£o E. Ferreira
We developed a database system for collaborative HIV analysis (DBCollHIV) in Brazil. The main purpose of our DBCollHIV project was to develop an HIV-integrated database system with analytical bioinformatics tools that would support the needs of Brazilian research groups for data storage and sequence analysis. Whenever authoriz.. Read More»
- Genet. Mol. Res. 5(1):
Predicting enzyme class from protein structure using Bayesian classification
Authors: Luiz C. Borro, Stanley R.M. Oliveira, Michel E.B. Yamagishi, Adaulto L. Mancini, Jos�© G. Jardine, Ivan Mazoni, Edgard H. dos Santos, Roberto H. Higa, Paula R. Kuser and Goran Neshich
Predicting enzyme class from protein structure parameters is a challenging problem in protein analysis. We developed a method to predict enzyme class that combines the strengths of statistical and data-mining methods. This method has a strong mathematical foundation and is simple to implement, achieving an accuracy of 45%. A c.. Read More»
- Genet. Mol. Res. 5(1):