Exploring molecular networks using MONET ontology
Authors: Jo�£o Paulo M�¼ller da Silva, Ney Lemke, Jos�© Carlos Mombach, Jos�© Guilherme Camargo de Souza, Marialva Sinigaglia and Renata Vieira
The description of the complex molecular network responsible for cell behavior requires new tools to integrate large quantities of experimental data in the design of biological information systems. These tools could be used in the characterization of these networks and in the formulation of relevant biological hypotheses. The .. Read More»
- Genet. Mol. Res. 5(1):
Analysis of slipped sequences in EST projects
Authors: Christian Baudet and Zanoni Dias
Slippage is an important sequencing problem that can occur in EST projects. However, very few studies have addressed this. We propose three new methods to detect slippage artifacts: arithmetic mean method, geometric mean method, and echo coverage method. Each method is simple and has two different strategies for processing seq.. Read More»
- Genet. Mol. Res. 5(1):
Karyotype of Brazilian Anopheles albitarsis sensu lato (Diptera:Culicidae)
Authors: MÃ?Âriam Silva Rafael, Ivanildo Pereira dos Santos-Junior, Wanderli Pedro Tadei, Maria Anice Mureb Sallum and Oswaldo Paulo Forattini
Anopheles (Nyssorhynchus) albitarsis sensu lato is an important malaria vector in Brazil, especially in the Brazilian Amazon region. Chromosome preparations of fourth-instar larvae of A. albitarsis from Iranduba and Coari (AM) and Ilha Comprida (SP) were analyzed for karyotype determination and to improve cytogenetic identific.. Read More»
- Genet. Mol. Res. 4(4):
Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection
Authors: Mariela Nieves, MarÃ?Âa MÃ?¼hlmann and Marta Dolores Mudry
Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome microdissection to develop a.. Read More»
- Genet. Mol. Res. 4(4):
Molecular phylogeny of penaeid shrimps inferred from two mitochondrial markers
Authors: Carolina M. Voloch, Pablo R. Freire and Claudia A.M. Russo
Penaeid shrimps are an important resource in crustacean fisheries, representing more than the half of the gross production of shrimp worldwide. In the present study, we used a sample of wideranging diversity (41 shrimp species) and two mitochondrial markers (758 bp) to clarify the evolutionary relationships among Penaeidae gen.. Read More»
- Genet. Mol. Res. 4(4):
Karyotypic characterization of Iheringichthys labrosus (Pisces, Pimelodidae): C-, G- and restriction endonuclease banding
Authors: Rafael Augusto de Carvalho and Ana L�ºcia Dias
Various chromosomal banding techniques were utilized on the catfish, Iheringichthys labrosus, taken from the Capivara Reservoir. C-banding regions were evidenced in telomeric regions of most of the chromosomes. The B microchromosome appeared totally heterochromatic. The restriction endonuclease AluI produced a banding pattern .. Read More»
- Genet. Mol. Res. 4(4):
Cytogenetic and genotoxic effects of the insecticides, imidacloprid and methamidophos
Authors: N. Ulku Karabay and M. Gunnehir Oguz
We examined the cytogenetic and genotoxic effects of the neonicotinoid insecticide imidacloprid and the organophosphate insecticide methamidophos, when administered alone or in combination. These insecticides were tested with the bone marrow chromosome aberration assay and micronucleus test in rats and by the bacterial mutatio.. Read More»
- Genet. Mol. Res. 4(4):
The use of RAPD to characterize Bipolaris sorokiniana isolates
Authors: Maria Viviane Gomes M�¼ller, Jos�© Carlos Germani and Sueli Teresinha Van Der Sand
Bipolaris sorokiniana is a phytopathogenic fungus causing diseases of cereal crops such as common root rot, the leaf spot disease, seedling blight, and black point of the grain. Random-amplified polymorphic DNA (RAPD) assay was used to investigate the genetic diversity of 20 isolates collected from different cultivars in wheat.. Read More»
- Genet. Mol. Res. 4(4):
Simplified preparation of a DNA ladder using PCR
Authors: T.-Y. Wang, L. Wang and F. Wang
Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1177
- DOI:
- 10.4238/vol10-3gmr1177
Genome wide cloning of maize meiotic recombinase Dmc1 and its functional structure through molecular phylogeny
Authors: F. Etedali, B. Baghban Kohnehrouz, M. Valizadeh, A. Gholizadeh and M.A. Malboobi
The development of meiotic division and associated genetic recombination paved the way for evolutionary changes. However, the secondary and tertiary structure and functional domains of many of the proteins involved in genetic recombination have not been studied in detail. We used the human Dmc1 gene product along with secondar.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1338
- DOI:
- 10.4238/vol10-3gmr1338