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Plant Genetics   Research Article

Variability among Capsicum baccatum accessions from Goi�¡s, Brazil, assessed by morphological traits and molecular markers

Authors: A.L.A. Martinez, J.S.P. Ara�ºjo, C.F. Ragassi, G.S.C. Buso and F.J.B. Reifschneider

Capsicum peppers are native to the Americas, with Brazil being a significant diversity center. Capsicum baccatum accessions at Instituto Federal (IF) Goiano represent a portion of the species genetic resources from central Brazil. We aimed to characterize a C. baccatum working collection comprising 27 accessions and 3 commerci.. Read More»

Genet. Mol. Res. 16(3):
gmr16039074
DOI:
10.4238/gmr16039074
Plant Genetics   Research Article

An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae)

Authors: S.R. Arruda, D.G. Pereira, M.M. Silva-Castro, M.G. Brito and A.M. Waldschmidt

Some species are characterized by a high content of tannins, alkaloids, and phenols in their leaves. These secondary metabolites are released during DNA extraction and might hinder molecular studies based on PCR (polymerase chain reaction). To provide an efficient method to extract DNA, Mimosa tenuiflora, an important legumino.. Read More»

Genet. Mol. Res. 16(3):
gmr16039063
DOI:
10.4238/gmr16039063
Human Genetics   Research Article

Association of endothelin-1 gene polymorphisms with essential hypertension in a Chinese population

Authors: Z. Fang, M. Li, Z. Ma and G. Tu

Endothelin-1 (ET-1) is the most potent endogenous vasoconstrictor and is involved in several vascular disorders such as hypertension. Its strong interaction with other vasoactive hormone systems suggests that the ET-1 gene (EDN1) is a potential candidate molecule that influences the risk of developing hypertension. Recently, t.. Read More»

Genet. Mol. Res. 16(3):
gmr16037446
DOI:
10.4238/gmr16037446
Plant Genetics   Research Article

Identification of Colletotrichum isolates from Capsicum chinense in Amazon

Authors: C.V.S. de Oliveira, K.S. Matos, D.M.C. de Albuquerque, R.E. Hanada and G.F. da Silva

Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colle.. Read More»

Genet. Mol. Res. 16(2):
gmr16029601
DOI:
10.4238/gmr16029601
Animal Genetics   Research Article

Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets

Authors: W.Y. Qin, L.N. Gan, R.W. Xia, W.H. Dong, S.Y. Sun, G.Q. Zhu,S.L. Wu and W.B. Bao

Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq res.. Read More»

Genet. Mol. Res. 16(2):
gmr16029574
DOI:
10.4238/gmr16029574
Plant Genetics   Research Article

Identification and characterization of the bZIP transcription factor involved in zinc homeostasis in cereals

Authors: A.R. Henriques, D. da R. Farias and A. Costa de Oliveira

Members of the basic leucine zipper family, as bZIP19, are considered to be essential regulators of the adaptation to zinc deficiency. Knowing that this gene as well as its targets are conserved in the plant kingdom, we followed an in silico approach to identify and characterize the bzip19 gene in cereals. Through BLASTp in Ph.. Read More»

Genet. Mol. Res. 16(2):
gmr16029558
DOI:
10.4238/gmr16029558
Plant Genetics   Research Article

Artificial neural networks as auxiliary tools for the improvement of bean plant architecture

Authors: V.Q. Carneiro, G.N. Silva, C.D. Cruz, P.C.S Carneiro, M. Nascimento and J.E.S. Carneiro

lassification using a scale of visual notes is a strategy used to select erect bean plants in order to improve bean plant architectures. Use of morphological traits associated with the phenotypic expression of bean architecture in classification procedures may enhance selection. The objective of this study was to evaluate the .. Read More»

Genet. Mol. Res. 16(2):
gmr16029500
DOI:
10.4238/gmr16029500
Human Genetics   Research Article

Mechanism of reversal of multidrug resistance by curcumin in human colorectal cancer cell line HCT-8/5-FU

Authors: Y.X. Fan, P. Abulimiti, H.L. Zhang, Y.K. Zhou and L. Zhu

Since there are no studies on the reversal of multidrug resistance by curcumin in the human colorectal cancer cell line HCT-8/5-FU, our aim was to search for highly efficient reversal agents and investigate the underlying mechanisms of this reversal. The cytotoxic effects of curcumin and 5-FU on HCT-8 and HCT-8/5-FU cells and .. Read More»

Genet. Mol. Res. 16(2):
gmr16029414
DOI:
10.4238/gmr16029414
Microbial Genetics   Research Article

Generic relationships among Molluginaceae inferred from a molecular phylogenetic analysis of the matK gene

Authors: M.A. Ali, J. Lee and F. Al-Hemaid

The family Molluginaceae (order Caryophyllales) is considered polyphyletic based on the photosynthetic pathway, C4 evolution, and phylogeny of the family. This inference was made based on photosynthetic, anatomical, and molecular datasets. The generic circumscription of this family has greatly been changed owing to the placeme.. Read More»

Genet. Mol. Res. 16(2):
gmr16029295
DOI:
10.4238/gmr16029295
Plant Genetics   Research Article

Genetic variability among elite popcorn lines based on molecular and morphoagronomic characteristics

Authors: J.F. dos Santos, C.A. Mangolin, M.F.P.S. Machado, C.A. Scapim, W. Giordani and L.S.A. Gon�§alves

Knowledge of genetic diversity among genotypes and relationships among elite lines is of great importance for the development of breeding programs. Therefore, the objective of this study was to evaluate genetic variability based on the morphoagronomic and molecular characterization of 18 elite popcorn (Zea mays var. everta) li.. Read More»

Genet. Mol. Res. 16(2):
gmr16029243
DOI:
10.4238/gmr16029243