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Plant Genetics   Research Article

Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey

Authors: G. Sar�±kam�±��1, F. Ya��ar2, M. Bak�±r3, K. Kazan4 and A. Erg�¼l3

Green bean genotypes collected from eastern Turkey were characterized using simple sequence repeat (SSR) markers and morphological traits. Among 12 SSR markers, 10 produced successful amplifications and revealed DNA polymorphisms that were subsequently used to assess genetic relatedness of the genotypes. Based on the number of alleles generated and the pr.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr605
DOI:
10.4238/vol8-3gmr605
Human Genetics   Research Article

Genetic polymorphisms of EGF 5'-UTR and NAT2 857G/A associated with glioma in a case control study of Malaysian patients

Authors: K.A. Muthusamy L.H. Lian N. Vairavan K.H. Chua V. Wara

Studies of genetic mutations that have been used in predicting glioma prognosis have revealed a complex relationship between clinical and genetic factors. Epidermal growth factor (EGF) and the NAT2 gene play a central role in carcinogenesis. An adenine (A) to guanine (G) single nucleotide polymorphism at position 61 in the 5'-untranslated region (5'-UTR) of .. Read More»

Genet. Mol. Res. 11(3):
2012.June.15.7
DOI:
10.4238/2012.June.15.7
Plant Genetics   Research Article

Comparison of coefficients and distance measurements in passion fruit plants based on molecular markers and physicochemical descriptors

Authors: C.B.M. Cerqueira-Silva1, C.B. Cardoso-Silva2, L.D.H.C.S. Concei�§�£o3, J.V.A. Nonato2, A.C. Oliveira2 and R.X. Corr�ªa3

We investigated seven distance measures and 14 simi­larity coefficients in a set of observations of variables of the ‘yellow’ passion fruit plant (Passiflora edulis Sims), submitted to multivari­ate analyses (distance, projection and grouping). Fourteen genotypes were characterized, based on DNA amplification with 16 random am­plif.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr614
DOI:
10.4238/vol8-3gmr614
Animal Genetics   Research Article

Doxycycline-regulated growth hormone gene expression system for swine

Authors: B.C. Jiang D.B. Yu L.J. Wang F.L. Dong H.A. Kaleri X.G. Wang N. Ally J. Li H.L. Liu

Although growth hormone gene transgenic animals are much larger than normal animals, they manifest differences that have adverse effects on survival due to overexpression of growth hormone. We developed a stable pig embryonic fibroblast cell line expressing pig growth hormone (pGH) using the Tet-On system, with which we can conditionally manipulate expressio.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.5
DOI:
10.4238/2012.July.10.5
Animal Genetics   Research Article

Effect of intracellular Wolbachia on interspecific crosses between Drosophila melanogaster and Drosophila simulans

Authors: I.N. Gazla and M.C. Carracedo

Wolbachia are bacteria that live inside the cells of a large number of invertebrate hosts and are transmitted from infected females to their offspring. Their presence is associated with cytoplasmic incompatibility in several species of Drosophila. Cytoplasmic incompatibility results when the sperm of infected males fertilize eggs of uninfected females, ca.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr595
DOI:
10.4238/vol8-3gmr595
Microbial Genetics   Research Article

Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara

Authors: Z.W. Zhang X.F. Shi M. Qu S.X. Ding

Because of their specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate animals. Previously, only two types of collectins had been isolated from bony fish: mannan-binding lectin (MBL) and galactose-binding lectin (GalBL). We sequenced a novel collectin (designated EALec1) from the red-spotted gro.. Read More»

Genet. Mol. Res. 11(3):
2012.May.11.1
DOI:
10.4238/2012.May.11.1
Human Genetics   Research Article

Assessing potential miRNA targets based on a Markov model

Authors: Hao-Yue Fu1,2, Ding-Yu Xue2, Xiang-de Zhang1 and Pei-Ying Yang2

At present, studies on microRNA mainly focus on the identification of microRNA genes and their mRNA targets. Although researchers have identified many microRNA genes, relatively few microRNA targets have been identified by experi­mental methods. Computational programs designed for predicting potential microRNA targets provide numerous targets for expe.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr604
DOI:
10.4238/vol8-3gmr604
Animal Genetics   Research Article

Behavior and viability of spontaneous oxidative stress-resistant Lactococcus lactis mutants in experimental fermented milk processing

Authors: M.N. Oliveira1, K.E. Almeida1, M.R. Damin1, T. Rochat2,J.-J. Gratadoux2, A. Miyoshi3, P. Langella2 and V. Azevedo3

Previously, we isolated two strains of spontaneous oxidative (SpOx2 and SpOx3) stress mutants of Lactococcus lactis subsp cremoris. Herein, we compared these mutants to a parental wild-type strain (J60011) and a commercial starter in experimental fermented milk production. Total solid contents of milk and fermen­tation temperature both affected the ac.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr635
DOI:
10.4238/vol8-3gmr635
Human Genetics   Research Article

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Authors: O.M.P. Duarte1, C.C.C. Martins1, A.M. Waldschmidt2 and M.A. Costa1

Scaptotrigona xanthotricha has a wide geographic distribution in the Brazilian Atlantic rainforest. One population from southeast and two from northeast Brazil were analyzed and were found to have chromosome polymorphisms. Although the chromo­some number 2n = 34 is conserved in this species, karyotypic analy­sis revealed clear differences between .. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr598
DOI:
10.4238/vol8-3gmr598
Human Genetics   Research Article

A novel NF1 mutation in a Chinese patient with giant caf�©-au-lait macule in neurofibromatosis type 1 associated with a malignant peripheral nerve sheath tumor and bone abnormality

Authors: H.-X. Tong M. Li Y. Zhang J. Zhu W.-Q. Lu

Neurofibromatosis type 1 (NF1; OMIM#162200) is a common neurocutaneous disorder that is characterized by multiple café-au-lait, skinfold freckling, Lisch nodules, and neurofibromas. Mutations in the NF1 gene, which encodes the neurofibromin protein, have been identified as the pathogenic gene of NF1. In this study, we present a clinical and molecul.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.6
DOI:
10.4238/2012.July.10.6