Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers
Authors: Y.S. Lin, C.S. Kuan, I.S. Weng and C.C. Tsai
The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.11
- DOI:
- 10.4238/2015.November.24.11
Expression of the bovine papillomavirus type 1, 2 and 4 L1 genes in the yeast Pichia pastoris
Authors: A.L.S. Jesus, F.C. Mariz, H.M. Souza, M.N. Cordeiro, E.C. Coimbra, M.C.G. Leit?£o, L.M. Nascimento, R.C. Stocco, W. Be?§ak and A.C. Freitas
Papillomaviruses are known to cause benign or malignant lesions in various animals. In cattle, bovine papillomavirus (BPV) is the etiologic agent of papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. Currently, there are no standard diagnostic tests or prophylactic vaccines. Protection against papillomavirus infection is.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.2
- DOI:
- 10.4238/2012.July.19.2
Structure and polymorphism of novel X-chromosome short tandem repeat loci in a Chinese Han population
Authors: Y.S. Zhu, H. Wu and J.H. Lai
Recently, 5 novel X-chromosome short tandem repeat (X-STR) loci with high degrees of polymorphism were examined. In this study, we investigated the genetic distribution of these loci in a Chinese Han population. The 5 X-STR loci were successfully examined by polyacrylamide gel electrophoresis in a total of 200 unrelated Shaanx.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.12
- DOI:
- 10.4238/2015.November.24.12
Effects of kinase insert domain receptor (KDR) gene silencing on the sensitivity of A549 cells to erlotinib
Authors: W.L. Zhu and Y.H. Liu
We investigated the effects of kinase insert domain receptor (KDR) gene silencing on the proliferation of A549 cells and their sensitivity to erlotinib. A KDR small interfering RNA (siRNA) sequence was designed and synthesized; then, it was transfected into A549 cells using LipofectamineTM 2000. KDR mRNA and protein expression after KDR gene si.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.15
- DOI:
- 10.4238/2015.November.24.15
Genetic structure in fragmented populations of Solanum lycocarpum A. St.-Hil. with distinct anthropogenic histories in a Cerrado region of Brazil
Authors: T.M. Moura, K. Martins, P.S. Sujii, A.M. Sebbenn and L.J. Chaves
Solanum lycocarpum is a woody tree widely distributed in the Cerrado that reaches high population densities in disturbed environments. We examined the genetic diversity and population differentiation of six S. lycocarpum populations with different degrees of human disturbance in order to determine if they are negatively affected by anthropogenic activity... Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.16
- DOI:
- 10.4238/2012.July.10.16
Analysis of an â??off-ladderâ? allele at the Penta D short tandem repeat locus
Authors: Y.L. Yang, J.G. Wang, D.X. Wang, W.Y. Zhang, X.J. Liu, J. Cao and S.L. Yang
Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.18
- DOI:
- 10.4238/2015.November.24.18
Effects of choice of month of treatment and parity order on bovine superovulation traits
Authors: Q. Deng, Y. Gao, C.H. Li1,, X.F. Yu, J.S. Ren, S.J. Li, C.Z. Chen, B. Yuan, Y. Ding, H. Jiang and J.B. Zhang
In this study, the performance of 300 Changbaishan Black cattle treated for superovulation from June to September was evaluated to determine the optimal conditions and herds for bovine embryo production. Data analysis revealed that cattle treated in July and August had higher numbers of available embryos (NAE), M1 embryos (NM1.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.14
- DOI:
- 10.4238/2015.November.24.14
Isolation and molecular characterization of a novel strain of Bacillus with antifungal activity from the sorghum rhizosphere
Authors: S.C. Martinez-Absalon, Ma. del C. Orozco-Mosqueda, M.M. Martinez-Pacheco, R. Farias-Rodriguez, M. Govindappa and G. Santoyo
We looked for bacterial strains with antifungal activity in the sorghum rhizosphere. A prescreening procedure to search for hemolytic activity among the isolated strains allowed us to detect good fungitoxic activity in a bacterial isolate that we named UM96. This bacterial isolate showed strong growth inhibition in bioassays against the pathogens Diaporth.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.15
- DOI:
- 10.4238/2012.July.10.15
Detection of CK19, LUNX, and KS1/4 mRNA expression in the peripheral blood for diagnosis of micrometastases in patients with non-small cell lung cancer and their clinical implications
Authors: L. Yan, Y. Yao, L.H. Wang, M.L. Wang and X.H. Fu
The expression of CK19, LUNX, and KS1/4 mRNA biomarkers was detected in the peripheral blood of non-small cell lung cancer (NSCLC) patients to investigate the feasibility of indicating lung cancer micrometastases. Micrometastases were identified in the peripheral blood of 32 NSCLC patients, 15 benign pulmonar.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.17
- DOI:
- 10.4238/2015.November.24.17
Molecular cloning and characterization of GbMECT and GbMECP gene promoters from Ginkgo biloba
Authors: S.Y. Cheng, L.L. Li, H.H. Yuan, F. Xu and H. Cheng
Ginkgolides are key pharmaceutical components in Ginkgo biloba. Using the cDNA sequence of the MECP and MECT genes to design primers, we obtained the promoters of these genes from Ginkgo genomic DNA using the genome walking method. The two promoters were 744 and 982 bp in length, respectively. The cis-element.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.20
- DOI:
- 10.4238/2015.November.24.20