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Animal Genetics   Research Article

Hematological markers and biochemical profiles in terms of gender and age of captive collared peccaries (Tayassu tajacu) in eastern Amazon

Authors: E.M. Jorge, C.J.O. Silva, R.A. Ritter, M.V.B. Monteiro, N.I. Albuquerque, P.R. Kahwage, F.O.B. Monteiro, C.T.C. Costa, S.C. Rahal and E. Silva Filho

Complete blood counts and blood biochemical analyses are laboratory tests that allow the monitoring of physiological condition, nutrition, and health in free-living or captive wild animals. When interpreting these tests, it is essential to compare the results with reference ranges that are suitable for the species. Few studies.. Read More»

Genet. Mol. Res. 14(4):
2015.November.24.7
DOI:
10.4238/2015.November.24.7
Bioinformatics   Research Article

Alteration of Phascolosoma esculenta heat shock protein 90 expression under heavy metal exposure and thermal stress

Authors: C.H. Li, X.R. Su, M.Q. Wang, Y.Y. Li, Y. Li, J. Zhou and T.W. Li

The full-length complementary DNA (cDNA) of heat shock protein 90 was cloned from Phascolosoma esculenta (PeHSP90) using expressed sequence tag and rapid amplification of cDNA end approaches. The cDNA of PeHSP90 was 2521 bp including a 5'-untranslated region of 110 bp, a 3'-untranslated region of 230 bp, and an open reading frame of 2181 bp. All of the ch.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.14
DOI:
10.4238/2012.July.10.14
Plant Genetics   Research Article

In vitro induction of tetraploid garlic with trifluralin

Authors: Z.-H. Cheng, X.-J. Zhou, M.A. Khan, L. Su and H.-W. Meng

Garlic (Allium sativum) is propagated asexually. Since sexual cross breeding is almost impossible, means for effective breeding are not currently available and the available production cultivars are seriously aged and degenerated. A possible alternative for breeding is chemical induction. Trifluralin, a type of herbicide, has been reported to provoke chro.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.13
DOI:
10.4238/2012.July.10.13
Microbial Genetics   Research Article

Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae

Authors: T. Jin, L. Zeng, Y.-Y. Lin, Y.-Y. Lu and G.-W. Liang

Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-tr.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.12
DOI:
10.4238/2012.July.10.12
Plant Genetics   Research Article

Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape

Authors: X.Q. Huang, R.Q. He, X.Y. Liao, B. Zhou, W.S. Peng, J.Z. Lin, D.Y. Tang, Y.H. Zhu, X.Y. Zhao and X.M. Liu

Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed ra.. Read More»

Genet. Mol. Res. 13(2):
Microbial Genetics   Research Article

Pyrroloquinoline quinone rescues hippocampal neurons from glutamate-induced cell death through activation of Nrf2 and up-regulation of antioxidant genes

Authors: Q. Zhang, M. Ding, X.R. Gao and F. Ding

Pyrroloquinoline quinone (PQQ) has been shown to protect primary cultured hippocampal neurons from glutamate-induced cell apoptosis by scavenging reactive oxygen species (ROS) and activating phosphatidylinositol-3-kinase (PI3K)/Akt signaling. We investigated the downstream pathways of PI3K/Akt involved in PQQ protection of glutamate-injured hippocampal ne.. Read More»

Genet. Mol. Res. 11(3):
2012.June.27.3
DOI:
10.4238/2012.June.27.3
Medical Genetics   Research Article

Genetic diversity and phylogeny of rhizobia isolated from Caragana microphylla growing in desert soil in Ningxia, China

Authors: J. Dai, X. Liu and Y. Wang

Rhizobia are soil bacteria with the capacity to induce nitrogen-fixing nodules on the roots or stems of legume plants. A total of 40 bacterial isolates from the root nodules of Caragana microphylla growing in desert soil in Ningxia, China, were analyzed for genetic diversity and phylogenetic position. These isolates were classified into 7 types of 16S rib.. Read More»

Genet. Mol. Res. 11(3):
2012.June.25.5
DOI:
10.4238/2012.June.25.5
Human Genetics   Research Article

Analysis of the BRAF V600E mutation in primary cutaneous melanoma

Authors: J.S.S. Inumaru1, K.I.F. Gordo, A.C. Fraga Junior, A.M.T.C. Silva, C.B.Q.S. Leal, F.M. Ayres, I.J. Wastowski, N.F. Borges and V.A. Saddi

BRAF V600E is the most common mutation in cutaneous melanomas, and has been described in 30-72% of such cases. This mutation results in the substitution of valine for glutamic acid at position 600 of the BRAF protein, which consequently becomes constitutively activated. The present study investigated the BRAF V600E mutation fr.. Read More»

Genet. Mol. Res. 13(2):
2014.January.22.8
DOI:
10.4238/2014.January.22.8
Plant Genetics   Research Article

Pericarp polypeptides and SRAP markers associated with fruit quality traits in an interspecific tomato backcross

Authors: J.H. Pereira da Costa, G.R. Rodr�­guez, G.R. Pratta, L.A. Picardi and R. Zorzoli

The aim of this study was to detect polypeptides and genomic regions associated with fruit quality traits in a backcross generation using as parent the Argentinean cultivated tomato Caimanta of Solanum lycopersicum and the wild accession LA722 of S. pimpinellifolium. We tested two types of molecular marker: polypeptide profile.. Read More»

Genet. Mol. Res. 13(2):
2014.January.24.10
DOI:
10.4238/2014.January.24.10
Bioinformatics   Research Article

Genetic diversity and population structure of Chinese pony breeds using microsatellite markers

Authors: L.X. Xu, S.L. Yang, R.Y. Lin, H.B. Yang, A.P. Li and Q.S. Wan

China is one of the principal origins of ponies in the world. We made a comprehensive analysis of genetic diversity and population structure of Chinese ponies based on 174 animals of five indigenous Chinese pony breeds from five provinces using 13 microsatellite markers. One hundred and forty-four alleles were detected; the mean number of effective allele.. Read More»

Genet. Mol. Res. 11(3):
2012.June.25.4
DOI:
10.4238/2012.June.25.4