Y. Wu

Association of CYP2C19*2 with hyperlipidemia in patients with the wild-type CYP2C9

• K. Khalighi
• G. Cheng
• B. Khalighi
• Y. Wu
• S. Mirabbasi
• W. Fan

Arachidonic acids are metabolized by cytochromes P450 (CYP450) to epoxyeicosatrienoic acids (EETs). EETs have many cardiovascular protective effects such as vasodilation, anti-hypertension, anti-inflammation, lipid-lowering effects, and favorable effects on glucose homeostasis. This study aimed to investigate whether gene polymorphism of cytochrome P450 CYP2C9 and C.. Read More»

Genet. Mol. Res. 16(3): gmr16039737

DOI: 10.4238/gmr16039737

Cloning and characterization of a �²-amyrin synthase gene from the medicinal tree Aralia elata (Araliaceae)

Y. Wu, H.D. Zou, H. Cheng, C.Y. Zhao, L.F. Sun, S.Z. Su, S.P. Li and Y.P. Yuan

Aralia elata is an important medicinal plant in China; it produces large amounts of oleanane type triterpene saponins. A full-length cDNA encoding β-amyrin synthase (designated as AeAS) was isolated from young leaves of A. elata by reverse transcription-PCR. The full-length cDNA of AeAS was found to have a 2292-bp open reading frame, encoding a protein with 763 amino acid residues. The ded.. Read More»

Genet. Mol. Res. 11(3): 2012.August.13.4

DOI: 10.4238/2012.August.13.4

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

N.M.A. Rahman, H.T. Fu, S.M. Sun, H. Qiao, S. Jin1,, H.K. Bai, W.Y. Zhang, G.X. Liang, Y.S. Gong, Y.W. Xiong and Y. Wu

Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of l-arginine to l-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the oriental river prawn Macrobrachium nipponen.. Read More»

Genet. Mol. Res. 15(3): gmr.15038541

DOI: 10.4238/gmr.15038541

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Q.N. Lei, Y.Y. Wu, H.Y. Liang, Z.X. Wang, Z. Zheng and Y.W. Deng

Small heat shock proteins (HSPs) are molecular chaperones with ATP-independent properties. They are involved in a variety of physiological and stress processes. In this study, the full-length HSP 20 (HSP20) from Pinctada martensii, designated as PmHSP20, was obtained from hemocytes using rapid amplification of cDNA ends technology. The PmHSP20 cDNA was 952 bp in len.. Read More»

Genet. Mol. Res. 15(2): gmr.15028799

DOI: 10.4238/gmr.15028799

MTA1 promotes cell proliferation via DNA damage repair in epithelial ovarian cancer

Q.Y. Yang1, J.H. Li2, Q.Y. Wang3, Y. Wu3, J.L. Qin3, J.J. Cheng3 and J. Qiu3

We examined whether metastasis-associated gene 1 (MTA1) promotes cell proliferation via DNA damage repair in ovarian cancer. MTA1 was successfully down-regulated using small interfering RNA in the epithelial ovarian cancer cell lines SKOV-3 and OVCAR-3. Cell growth was evaluated through MTT and colony formation assays. Fluorescence-activated cell sorting analysis was used to evaluate the distri.. Read More»

Genet. Mol. Res. 13(4):

Interleukin-10 promoter polymorphisms associated with susceptibility to lumbar disc degeneration in a Chinese cohort

W.P. Lin, J.H. Lin, X.W. Chen, C.Y. Wu, L.Q. Zhang, Z.D. Huang and J.M. Lai

We investigated a possible association between interleukin (IL)-10 single nucleotide polymorphisms (SNPs) and susceptibility to and severity of lumbar disc degeneration (LDD) in a Chinese cohort of 320 patients with LDD and 269 gender- and age-matched controls. The degree of disc degeneration was determined by magnetic resonance imaging using Schneiderman’s cl.. Read More»

Genet. Mol. Res. 10(3): vol10-3gmr1283

DOI: 10.4238/vol10-3gmr1283

Anticancer activity of Bombyx batryticatus ethanol extract against the human tumor cell line HeLa

W.P. Wu, J. Cao, J.Y. Wu, H. Chen and D. Wang

Anticancer activity of Bombyx batryticatus ethanol extract (BBE) against HeLa cells was studied using cell viability, DNA fragmentation, real-time polymerase chain reaction, and Western blot analyses. The BBE inhibited the growth and induced apoptosis of HeLa cells. The MTT assay indicated that the BBE induced cytotoxicity in HeLa cells in a time- and concentration-dependent manner. When HeLa c.. Read More»

Genet. Mol. Res. 14(1): 2015.January.15.10

DOI: 10.4238/2015.January.15.10

Meta-analysis demonstrates no association between p53 codon 72 polymorphism and prostate cancer risk

M.S. Li, J.L. Liu, Y. Wu, P. Wang and H. Teng

We examined whether p53 codon 72 polymorphism confers prostate cancer risk by conducting a meta-analysis. Two investigators independently searched the Pubmed, Embase and CBM databases. This meta-analysis was made of seven case-control studies, that included 892 prostate cancer cases and 1020 healthy controls. Meta-analysis results based on all the studies showed no .. Read More»

Genet. Mol. Res. 10(4): 2011.November.29.3

DOI: 10.4238/2011.November.29.3

Association between the XPG gene Asp1104His polymorphism and lung cancer risk

B. Zhou, X.M. Hu and G.Y. Wu

It has been suggested that the xeroderma pigmentosum complementation group G (XPG) gene Asp1104His polymorphism is linked to susceptibility to lung cancer. However, the results from the published studies are contradictory rather than conclusive. With this meta-analysis, we aimed to achieve a better understanding of the effects of the XPG gene Asp1104His polymorphism.. Read More»

Genet. Mol. Res. 15(2): gmr.15027395

DOI: 10.4238/gmr.15027395

Functional analysis of differentially expressed genes associated with glaucoma from DNA microarray data..

Y. Wu, W.D. Zang and W. Jiang

Microarray data of astrocytes extracted from the optic nerves of donors with and without glaucoma were analyzed to screen for differentially expressed genes (DEGs). Functional exploration with bioinformatic tools was then used to understand the roles of the identified DEGs in glaucoma. Microarray data were downloaded from the Gene Expression Omnibus (GEO) database, which contains 13 astrocyte s.. Read More»

Genet. Mol. Res. 13(4):