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Publications of : X.G. Wang
Plant Genetics   Research Article

Molecular identification of variety purity in a cotton hybrid with unknown parentage using DNA-SSR markers

Molecular identification of hybrid purity is difficult in regional trials of cotton varieties and hybrid trials. In particular, the molecular detection of hybrid purity has not yet been reported in the case of unknown parentage. In this study, we screened 5000 pairs of primers and chose 17 pairs of core simple sequence repeat (SSR) primers to determine the F1 purity.. Read More»

Genet. Mol. Res. 16(3): gmr16039799

DOI: 10.4238/gmr16039799

Medical Genetics   Research Article

De novo DNA methylation of the paternal genome in 2-cell mouse embryos

    X.S. Ma1, X.G. Wang1,2, L. Qin1, C.L. Song1, F. Lin1, J.M. Song1, C.C. Zhu1 and H.L. Liu

The developmental dynamics of DNA methylation events have been well studied. Active demethylation of the paternal genome occurs in the zygote, passive demethylation occurs during cleavage stages, and de novo methylation occurs by the blastocyst stage. It is believed that the paternal genome has lower levels of methylation during early development than the maternal genome. However, in this study.. Read More»

Genet. Mol. Res. 13(4):

Animal Genetics   Research Article

Expression and Localization of Luman/CREB3 in Mouse Embryos during the Pre-implantation period - Genet. Mol. Res. 14 (4): 13595-13602.

    T.N. Mahmoud, P.F. Lin, F.L. Chen, J.H. Zhou, X.G. Wang, N. Wang, X. Li andY.P. Jin

In regard to the affiliation mistake that was made during our correspondence in our manuscript, we write this letter in order to correct it as per my PhD requirements and this is the Final decision as I have no other option. The correction is only in the affiliation and should be: T.N. Mahmoud1,2, P.F. Lin1, F.L. Chen1, J.H. Zhou1, X.G. Wang1, N. Wang1, X. Li1 and Y.P. Jin1. 1Key Laboratory of .. Read More»

Genet. Mol. Res. 15(1): gmr.150156091

DOI: 10.4238/gmr.150156091

Animal Genetics   Research Article

Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development

    B.J. Wu, F.L. Dong, X.S. Ma, X.G. Wang, F. Lin and H.L. Liu

Epigenetic modifications of the genome, such as histone H2A variants, ensure appropriate gene activation or silencing during oogenesis and preimplantation embryo development. We examined global localization and expression of the histone H2A variants, including H2A.Bbd, H2A.Z and H2A.X, during mouse oogenesis and preimplantation embryo development. Immunocytochemistr.. Read More»

Genet. Mol. Res. 13(3): 2014.August.7.8

DOI: 10.4238/2014.August.7.8

Human Genetics   Research Article

Identification of a locus characteristic of male individuals of buffalo grass [Buchloe dactyloides (Nutt.) Engelm.] by using an RAPD marker

    Y.X. Li, X.G. Wang, C.H. Yang, L.L. Cong, F.F. Wu, J.G. Xue and Y.H. Han

Buffalo grass [Buchloe dactyuloides (Nutt.) Engelm.] plants can be either male, female, or hermaphrodite (monoecious). As there is no morphological difference in the early vegetative growth of these three classes of plants, it is worthwhile to use molecular biological methods to attempt to identify the sex of a plant at this early growth period. In this study, we identified 23 plants that had a.. Read More»

Genet. Mol. Res. 12(3): 2013.September.27.8

DOI: 10.4238/2013.September.27.8

Microbial Genetics   Research Article

Silencing effect of lentiviral vectors encoding shRNA of Herp on endoplasmic reticulum stress and inflammatory responses in RAW 264.7 macrophages

    F.L. Chen, Q. Li, J.Y. Zhang1,, L.J. Lei, Z. Zhang, T.N. Mahmoud, X.G. Wang, P.F. Lin, Y.P. Jin and A.H. Wang

Herp, a mammalian protein with a ubiquitin-like domain, can be strongly upregulated by endoplasmic reticulum (ER) stress during ER-associated protein degradation. However, the other cellular functions of Herp remain unclear. We explored the effect of Herp on ER stress and inflammatory responses in RAW 264.7 macrophages that had been exposed to tunicamycin or thapsigargin. We successfully constr.. Read More»

Genet. Mol. Res. 14(4): 2015.December.21.31

DOI: 10.4238/2015.December.21.31

Animal Genetics   Research Article

Expression and localization of Luman/CREB3 in mouse embryos during the pre-implantation period

    T.N. Mahmoud, P.F. Lin, F.L. Chen, J.H. Zhou, X.G. Wang, N. Wang, X. Li and Y.P. Jin

Luman/CREB3 is a transcription factor that is a member of the cAMP-response-element-binding protein family of basic region-leucine zipper transcription factors. This protein interacts with host cell factor 1, which also associates with the herpes simplex virus protein VP16 to induce the transcription of herpes simplex virus. Currently, the physiological function of Luman/CREB3 in reproductive p.. Read More»

Genet. Mol. Res. 14(4): 2015.October.28.20

DOI: 10.4238/2015.October.28.20

Animal Genetics   Research Article

Doxycycline-regulated growth hormone gene expression system for swine

    B.C. Jiang D.B. Yu L.J. Wang F.L. Dong H.A. Kaleri X.G. Wang N. Ally J. Li H.L. Liu

Although growth hormone gene transgenic animals are much larger than normal animals, they manifest differences that have adverse effects on survival due to overexpression of growth hormone. We developed a stable pig embryonic fibroblast cell line expressing pig growth hormone (pGH) using the Tet-On system, with which we can conditionally manipulate expression of pGH in vivo. Inducible expression o.. Read More»

Genet. Mol. Res. 11(3): 2012.July.10.5

DOI: 10.4238/2012.July.10.5

Human Genetics   Research Article

Identification of CD14 transcript in blood polymorphonuclear neutrophil leukocytes and functional variation in Holsteins

    J.M. Huang, X.G. Wang, Q. Jiang, Y. Sun, C.H. Yang, Z.H. Ju,H.S. Hao, C.F. Wang, J.F. Zhong and H.B. Zhu

Polymorphonuclear neutrophil (PMN) leukocytes are primary phagocytic cells of the bovine mammary gland and a first line of defense against invading pathogens during bovine mastitis infection. Cluster of differentiation 14 (CD14) is mainly expressed in macrophages and neutrophils and acts as a co-receptor that binds bacterial lipopolysaccharide (LPS) and recruits PMN.. Read More»

Genet. Mol. Res. 15(2): gmr.15027932

DOI: 10.4238/gmr.15027932

Human Genetics   Research Article

A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression

    H. Li, Q. Jiang, Z.H. Ju, J.M. Huang, X.G. Wang, C.H. Yang, Y. Sun, G.W. Yang, J.F. Zhong and C.F. Wang

The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain reaction (RT-PCR) and clone sequencing method.. Read More»

Genet. Mol. Res. 15(2): gmr.15027723

DOI: 10.4238/gmr.15027723

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