X. Chen

Effect of Shenqin biochemical extract on hypoxia-inducible factor-1α expression in ultraviolet B-irradiated HaCaT cells

• X. Chen
• J. Bai
• Y. Li
• X.-G. Li
• G.-F. Lv
• H.-Ch. Zhang

Hypoxia-inducible factor-1α (HIF-1α) is considered the main transcriptional regulator of the hypoxia-specific cellular and developmental response. This study was performed to investigate the effect of Shenqin biochemical extract (SQBE) on HIF-1α expression in ultraviolet B (UVB)-irradiated HaCaT cells and the possible action mechanisms of SQBE agai.. Read More»

Genet. Mol. Res. 16(2): gmr16029430

DOI: 10.4238/gmr16029430

Expression and clinical significance of obesity-associated gene STEAP4 in obese children

H.M. Xu, Y.Z. Cui, W.G. Wang, H.X. Cheng, Y.J. Sun, H.Y. Zhao and Y.Q. Yan

The aim of this study was to investigate the expression and clinical significance of the obesity-associated gene STEAP4 in obese children. Fifty-three obese children and 33 children with a standard body weight (control) from our hospital were recruited to this study. The expression of STEAP4 mRNA and protein in the adipose tissue were detected by reverse transcripta.. Read More»

Genet. Mol. Res. 15(4): gmr.15048705

DOI: 10.4238/gmr.15048705

Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip

M.Z. Zhang, X.F. Zhang, X.M. Chen, X. Chen, S. Wu and L.L. Xu

The enzyme-linked probe hybridization chip utilizes a method based on ligase-hybridizing probe chip technology, with the principle of using thio-primers for protection against enzyme digestion, and using lambda DNA exonuclease to cut multiple PCR products obtained from the sample being tested into single-strand chains for hybridization. The 5'-end amino-labeled probe was fixed onto the aldehyde.. Read More»

Genet. Mol. Res. 14(3): 2015.August.10.10

DOI: 10.4238/2015.August.10.10

Dynamic QTL analysis of seed reserve utilization in sh2 sweet corn germination stages

X.X. Cheng, S. He1 and G.H. Geng

In this study, dynamic quantitative trait loci (QTL) were mapped in a recombinant inbred line (F2:4) population derived from a BF3109 x Q267 cross. This was done during the unconditional (4, 7, and 10 days) and conditional (0 to 4, 4 to 7, and 7 to 10 days) germination stages in sh2 sweet corn. The values of seedling dry weight, weight of mobilized seed reserve (WMSR), seed reserve depletion pe.. Read More»

Genet. Mol. Res. 15(3): gmr.15038183

DOI: 10.4238/gmr.15038183

Hepatitis B virus X protein activates human hepatic stellate cells through upregulating TGFβ1

H.-Y. Chen, Z.-X. Chen, R.-F. Huang, N. Lin and X.-Z. Wang

We investigated the effects of the hepatitis B virus X gene (HBV X) on the activation of human hepatic stellate cells (HSCs) and the possible mechanisms underlying the pathway. Recombinant plasmid pHBV-X-IRES2-EGFP was constructed and transfected into HL-7702 cells using a lipid-mediated method. Transfected cells were screened by G418, which detected stable expression of the X gene by reverse t.. Read More»

Genet. Mol. Res. 13(4):

Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone

R. Liu, H.H. Zhang, Z.X. Chen, M.Q. Shahid, X.L. Fu and X.D. Liu

Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was selected as a probe to screen the O. offi.. Read More»

Genet. Mol. Res. 14(4): 2015.October.28.29

DOI: 10.4238/2015.October.28.29

Protective effect of lyophilized recombinant human brain natriuretic peptide on renal ischemia/reperfusion injury in mice

X. Cao, H.Y. Xia, T. Zhang, L.C. Qi, B.Y. Zhang, R. Cui, X. Chen, Y.R. Zhao and X.Q. Li

Brain natriuretic peptide (BNP) has a protective effect on acute injury of the heart, brain, and lung. However, its role in acute kidney injury (AKI) remains unclear. The aim of this study was to investigate the effect of lyophilized recombinant human BNP (lrh-BNP) on AKI and the underlying molecular mechanisms. An experimental model for AKI was established using an ischemia/reperfusion (I/R) p.. Read More»

Genet. Mol. Res. 14(4): 2015.October.26.26

DOI: 10.4238/2015.October.26.26

Polymorphisms of the angiotensin II type 1 receptor gene affect antihypertensive response to angiotensin receptor blockers in hypertensive Chinese

H.T. Gong, X.L. Ma, B.X. Chen, X.Y. Xu, Q. Li, C.X. Guo and F.H. Du

The renin-angiotensin-aldosterone system plays a key role in regulating blood pressure by maintaining vascular tone and the water/sodium balance. Many antihypertensive drugs target the renin-angiotensin-aldosterone system, but the effect differs considerably among hypertensive patients. We investigated whether genetic variants of the angiotensin II type 1 receptor are associated with blood pres.. Read More»

Genet. Mol. Res. 12(2): 2013.June.21.2

DOI: 10.4238/2013.June.21.2

Genetic diversity of HLA-DRB1 alleles in the Tujia population of Wufeng, Hubei Province, China

X.P. Qiu, L. Zhang, F.Y. Zeng, Y. Wen, C. Li, L.X. Qiu, D.X. Cheng and X.X. Wu

We established a genetic database by investigating human leukocyte antigen (HLA)-DRB1 allelic frequencies in a disease-association study in the Tujia population in Wufang, Hubei, China. The allele frequencies of the HLA-DRB1 locus in 262 healthy, unrelated Tujia individuals living in the Wufeng region of the Hubei Province were analyzed using the Luminex HLA sequenc.. Read More»

Genet. Mol. Res. 13(3): 2014.July.2.5

DOI: 10.4238/2014.July.2.5

Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep

Y. Yue, X. Cheng, S.G. Zhao, Z. Liu, L.S. Liu, R. Zhou, J.P. Wu and M.A. Brown

To evaluate stearoyl-CoA desaturase (SCD), hormone-sensitive lipase (HSL), lipoprotein lipase (LPL), and peroxisome proliferator-activated receptor (PPARγ) expression in Lanzhou fat-tailed sheep (with and without docked tails), 18 rams were randomly divided into two equal groups (docked group, LT; control group, LC). These data were also used to increase the u.. Read More»

Genet. Mol. Res. 15(1): gmr.15017323

DOI: 10.4238/gmr.15017323