In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information con.. Read More»
Genet. Mol. Res. 16(3): gmr16039712
DOI: 10.4238/gmr16039712
We determined whether salubrinal can protect cardiomyocytes from doxorubicin-induced apoptosis and explored the related mechanisms to provide experimental evidence for exploring novel drug candidates to decrease cardiac toxicity. Neonatal rat cardiomyocytes were isolated, cultured in vitro, and pretreated with salubrinal (10, 20, or 40 μM) to observe their r.. Read More»
Genet. Mol. Res. 14(4): 2015.October.16.4
DOI: 10.4238/2015.October.16.4
Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a rare, autosomal recessive disorder associated with mutations in the thymidine phosphorylase (TYMP) gene. The main objective of this study was to characterize the genetic profiles of the deceased proband’s family members (N = 4) using DNA sequencing and to determine miRNA deregulation in MNGIE using miRNA microarray profili.. Read More»
Genet. Mol. Res. 14(4): 2015.October.26.13
DOI: 10.4238/2015.October.26.13
Formalin fixation and paraffin embedding is widely used for convenient and long-term storage of tumor tissue and precious sources to perform genetic studies. However, DNA fragmentation is one of the major flaws of genomic DNA isolation from formalin fixation tissues, which limits its further usage. Here, we present an improved method for isolating high-quality genomic DNA from formalin fixation.. Read More»
Genet. Mol. Res. 15(3): gmr.15037972
DOI: 10.4238/gmr.15037972
We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructed, which localized to the plasma membran.. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.May.9.4
DOI: http://dx.doi.org/10.4238/2014.May.9.4
The receptors for the immunosuppression drugs FK506 and rapamycin are called FKBPs (FK506-binding proteins). FKBPs comprise a large family; they are found in many species, including bacteria, fungi, animals, and plants. As a class of peptidyl-prolyl cis-trans isomerase enzymes, the FKBP genes have been the focus of recent studies on plant stress tolerance and immuno.. Read More»
Genet. Mol. Res. 11(2): 2012.June.25.2
DOI: 10.4238/2012.June.25.2
We investigated the effect of propofol on the proliferation and viability of rat embryonic neural stem cells (rENSCs) and the potential mechanisms involved. rENSCs were isolated and cultured in vitro and treated with 1, 10, or 50 μM propofol, while the control group was treated with 0.1 μM dimethyl sulfoxide. The effect of propofol on the proliferatio.. Read More»
Genet. Mol. Res. 14(4): 2015.November.18.57
DOI: 10.4238/2015.November.18.57
A drought-induced gene, DIP3, encoding a chitinase III protein was isolated from the roots of upland rice by reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis demonstrated that the cDNA and deduced protein showed high identity to Oryza sativa class III chitinase. The deduced protein contained a signal peptide sequence in the N-terminal region of 21aa and a conserved gl.. Read More»
Genet. Mol. Res. 12(4): 2013.December.19.5
DOI: 10.4238/2013.December.19.5
Cardiovascular and cerebrovascular diseases (CCVDs) are common and have high rates of morbidity, mortality, and recurrence. Thrombin-activatable fibrinolysis inhibitor (TAFI) is also known as carboxypeptidase B2 and is encoded by the CPB2 gene; CPB2 polymorphisms have been explored in a variety of studies, but their correlation to the risk of CCVDs remains ambiguous.. Read More»
Genet. Mol. Res. 15(2): gmr.15026718
DOI: 10.4238/gmr.15026718
Activity of methylenetetrahydrofolate reductase (MTHFR), an enzyme involved in folate metabolism, is influenced by mutations in the corresponding gene, contributing to a decrease in 5,10-MTHF. Due to such polymorphisms, individuals differ in MTHFR enzyme activity and plasma folate levels. We investigated the relationship between two common MTHFR polymorphisms (C677T and A1298C) and breast cance.. Read More»
Genet. Mol. Res. 15(3): gmr.15038679
DOI: 10.4238/gmr.15038679
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