F.F. Chen

Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus)

• F.F. Chen
• C.Y. Ma
• L.P. Yan
• H. Zhang
• W. Wang
• Y. Zhang
• L.B. Ma

In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information con.. Read More»

Genet. Mol. Res. 16(3): gmr16039712

DOI: 10.4238/gmr16039712

N-terminal functional region of the invariant chain efficiently targets the binding of a CTL epitope to MHC class I molecules during cross-presentation

C. Wu, D.G. Zhang, F.F. Chen, X.L. Liu, S.J. Liu and W.Y. Yu

Cross-presentation (CP) is important for priming T cell responses to many viral, bacterial, and tumor antigens. Here, we designed two Ii mutants, based on evidence that the invariant chain (Ii, also named CD74) binds newly synthesized MHC class I molecules with the class II-associated invariant chain peptide (CLIP) region of Ii, which occupies the peptide-binding gr.. Read More»

Genet. Mol. Res. 13(2): 2014.April.3.16

DOI: 10.4238/2014.April.3.16

Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata)

S.J. Liu, F.F. Chen, Y. Dai, C. Wu, Q.S. Ni and W.Y. Yu

The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of cDNA ends (RACE)-PCR and by measuring e.. Read More»

Genet. Mol. Res. 10(4):

Molecular characterization and tissue-specific expression of invariant chain isoform in Muscovy Duck (Cairina moschata)

S.J. Liu1,2, F.F. Chen2, C. Wu2, Q.S. Ni2 and W.Y. Yu2

Invariant chain (Ii) isoform, through its thyroglobulin-like (Tg) domain, inhibits cysteine proteases during antigen presentation in vertebrates. In birds, the Ii of Muscovy Duck (MDIi) has 2 forms: MDIi-1 and MDIi-2 (MDIi isoform). To understand the genetic information and expression characteristics of MDIi-2, polymerase chain reaction, and bioinformatic analysis were performed for MDIi-2 from.. Read More»

Genet. Mol. Res. 13(4):