M.M. Franco

Study of genetic variability in pigs after the traditional breeding program

• I.M. Ferreira
• G.S. Vieira
• T.F. Braga
• T.C.F. Silva
• M.M. Franco
• R.C. Antunes

Molecular markers are tools used to improve genetic gains. The objective of this study was to analyze the security of alleles of molecular marker genes for characteristics of economic interest in a pure population of pigs. After the extraction of DNA from the hair of 272 Large White matrices, the allele and genotype frequency of single nucleotide polymorphism was pe.. Read More»

Genet. Mol. Res. 16(3):

Quick method for identifying horse (Equus caballus) and donkey (Equus asinus) hybrids

M.M. Franco, J.B.F. Santos, A.S. Mendonça, T.C.F. Silva, R.C. Antunes and E.O. Melo

The domestication of the Equus genus 5000-6000 years ago has influenced the history of human civilization. As soon as horse and donkey species had been domesticated, they were crossbred, producing humanity’s first documented attempt at animal genome manipulation. Since then, the mule (male donkey x female horse) and the reciprocal cross (the hinny, male horse x female donkey) have been th.. Read More»

Genet. Mol. Res. 15(3): gmr.15038895

DOI: 10.4238/gmr.15038895

Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer

A.R. Ferreira, L.F.C. Aguiar Filho, R.V. Sousa, R. Sartori and M.M. Franco

Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Total RNA was isolated from individual biops.. Read More»

Genet. Mol. Res. 14(4): 2015.October.5.26

DOI: 10.4238/2015.October.5.26

Changes in gene expression profiles of bovine embryos produced in vitro, by natural ovulation, or hormonal superstimulation

T.C.D. Mundim, A.F. Ramos, R. Sartori, M.A.N. Dode, E.O. Melo, L.F.S. Gomes, R. Rumpf and M.M. Franco

Embryos produced by hormonal superstimulation have been used as an in vivo control in most published research on embryo gene expression. However, it is not known if this is the most appropriate control for gene expression profile studies. We compared the expression of GRB-10, IGF-II, IGF-IIR, MnSOD, GPX-4, catalase, BAX, and interferon-τ genes, in embryos produc.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr646

DOI: 10.4238/vol8-4gmr646