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T.F. Braga

Publications of : T.F. Braga
Animal Genetics   Research Article

Study of genetic variability in pigs after the traditional breeding program

Molecular markers are tools used to improve genetic gains. The objective of this study was to analyze the security of alleles of molecular marker genes for characteristics of economic interest in a pure population of pigs. After the extraction of DNA from the hair of 272 Large White matrices, the allele and genotype frequency of single nucleotide polymorphism was pe.. Read More»

Genet. Mol. Res. 16(3):

Plant Genetics   Research Article

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

    A.C.P. Silveira, R.C. Antunes, J.F. Almeida, T.F. Braga, P.F.A. Freitas, A.S.M. César and E.C. Guimarães

The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fragment length polymorphism using the rest.. Read More»

Genet. Mol. Res. 7(4): vol7-4gmr511

DOI: 10.4238/vol7-4gmr511

Animal Genetics   Research Article

PIT1 gene polymorphism in Pietrain and Large White pigs after divergent selection

    A.C.P. Silveira1, T.F. Braga1, J.F. Almeida2, R.C. Antunes1,P.F.A. Freitas1, A.S.M. Cesar1 and E.C. Guimarães1

We examined the polymorphisms in the PIT1 gene of 103 pigs and compared their frequencies in the maternal and paternal lineages of the Pietrain and Large White breeds, which have undergone divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by PCR and genotyped by RFLP, using the restriction enzyme RsaI. The data were analyzed with the chi.. Read More»

Genet. Mol. Res. 8(3): vol8-3gmr609

DOI: 10.4238/vol8-3gmr609