M.F.P.S. Machado
Genetic variability among elite popcorn lines based on molecular and morphoagronomic characteristics
Knowledge of genetic diversity among genotypes and relationships among elite lines is of great importance for the development of breeding programs. Therefore, the objective of this study was to evaluate genetic variability based on the morphoagronomic and molecular characterization of 18 elite popcorn (Zea mays var. everta) lines to be used by Universidade Estadual .. Read More»
Genet. Mol. Res. 16(2): gmr16029243
DOI: 10.4238/gmr16029243
Genetic diversity of popcorn genotypes using molecular analysis
In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were monomorphic and 92 were polymorphic. To cal.. Read More»
Genet. Mol. Res. 14(3): 2015.August.19.16
DOI: 10.4238/2015.August.19.16
Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome
Amplified fragment length polymorphism (AFLP) analysis was used to evaluate DNA polymorphism in Pilosocereus gounellei with the aim of differentiating samples grown in different Brazilian semiarid regions. Seven primer pairs were used to amplify 703 AFLP markers, of which 700 (99.21%) markers were polymorphic. The percentage of polymorphic markers ranged from 95.3% for the primer combination E-.. Read More»
Genet. Mol. Res. 14(4): 2015.December.15.2
DOI: 10.4238/2015.December.15.2
Genetic diversity analysis of Capsicum spp germplasm bank accessions based on �?±/�?²-esterase polymorphism
Genetic diversity and structure were analyzed in 10 accessions belonging to Banco Ativo de Germoplasma de Capsicum located at Federal University of Piauí in northwestern Brazil that receives pepper samples grown in community gardens in various regions and Brazilian states. Selections were made from seeds of C. chinense (4 accessions), C. annuum (5 accessions), and C. baccatum (1 accessio.. Read More»
Genet. Mol. Res. 12(2): 2013.April.12.2
DOI: 10.4238/2013.April.12.2
Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses
This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with a-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.8S-ITS2 sequencing data were used as rD.. Read More»
Genet. Mol. Res. 12(3): 2013.September.27.11
DOI: 10.4238/2013.September.27.11
Genetic divergence among sweet corn lines estimated by microsatellite markers
The purpose of this study was to analyze the genetic diversity of 15 sugary-1 sweet corn lines by microsatellite markers. One hundred pairs of simple sequence repeat primers that were mapped for field corn were tested. Of these primers, 15% were polymorphic, and all were selected for the evaluation. These primers identified a total of 39 alleles among the 15 loci that were evaluated... Read More»
Genet. Mol. Res. 13(4): 2014.December.12.3
DOI: 10.4238/2014.December.12.3
Genetic divergence and admixture of ancestral genome groups in the sugarcane variety ��?RB867515��? (Saccharum spp
We analyzed 80 plants of the sugarcane (Saccharum spp) variety ‘RB867515’ in order to investigate its diversity and genetic structure at the molecular level. Four simple sequence repeat (SSR) loci (UGSM51, SMC1237, SEGMS1069, and UGSM38) and five expressed sequence tag (EST)-SSR loci (ESTA68, ESTB92, ESTB145, ESTC66, and ESTC84) were used as molecular ma.. Read More»
Genet. Mol. Res. 15(4): gmr15049209
DOI: 10.4238/gmr15049209
Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA
Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism and to examine relationships among four table grape clones from northwestern Paraná, in southern Brazil. The 10 primers used for RAPD fingerprints generated 126 reproducible fragments, of which 63, 68, 76, and 72 were polymorphic in cultivars Italia, Rubi, Benitaka, and Brasil, r.. Read More»
Genet. Mol. Res. 8(1): vol8-1gmr536
DOI: 10.4238/vol8-1gmr536
Esterase polymorphisms for analysis of genetic diversity and structure of soybean (Glycine max) cultivars
We used native polyacrylamide gel electrophoresis to identify polymorphism levels in a- and b-esterase loci from leaf tissues of Brazilian soybean cultivars for the analysis of population genetic diversity and structure, and to investigate relationships between conventional and genetically modified cultivars. The cultivars included lines developed by a soybean-growe.. Read More»
Genet. Mol. Res. 10(4): 2011.October.13.4
DOI: 10.4238/2011.October.13.4
Vegetative compatibility and parasexual segregation in Colletotrichum lindemuthianum, a fungal pathogen of the common bean
The heterokaryotic and vegetative diploid phases of Colletotrichum lindemuthianum are described using nutritional and biochemical markers. Nitrate non-utilizing mutants (nit), derived from R2047, R89, R73, R65, and R23 isolates, were paired in all possible combinations to obtain heterokaryons. Although pairings R2047/R89, R2047/R73, R65/R73, and R73/R23 showed compl.. Read More»
Genet. Mol. Res. 6(3):
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