D.A. Oliveira
Structure and genetic diversity of Anacardium humile (Anacardiaceae): a tropical shrub
Anacardium humile Saint Hilaire is a tropical shrub native to the Cerrado biome. It is a fruiting species with biological, medicinal, and socioeconomic significance. Thus, knowing how the genetic variability of natural populations is organized allows for the establishment of strategies for conservation and the sustainable use of the species and its biome. Six micros.. Read More»
Genet. Mol. Res. 16(3): gmr16039778
DOI: 10.4238/gmr16039778
Leaf age affects the quality of DNA extracted from Dimorphandra mollis (Fabaceae), a tropical tree species from the Cerrado region of Brazil
Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in co.. Read More»
Genet. Mol. Res. 10(1): vol10-1gmr1030
DOI: 10.4238/vol10-1gmr1030
Standardization of a molecular method for epidemiologic identification of Leishmania strains
Molecular studies of the evolutionary relationships among Leishmania species suggest the presence of high genetic variation within this genus, which has a direct effect on public health in many countries. The coexistence of species in a particular region can result in different leishmaniasis clinical forms and treatment responses. We aimed to standardize the kinetop.. Read More»
Genet. Mol. Res. 15(4): gmr.15048854
DOI: 10.4238/gmr.15048854
Genetic markers for detection of Escherichia coli K-12 harboring ampicillin-resistance plasmid from an industrial wastewater treatment effluent pond
Biotechnology industries that use recombinant DNA technology are potential sources for release of genetically modified organisms to the environment. Antibiotic-resistance marker genes are commonly used for recombinant bacteria selection. One example is the marker gene coding for β-lactamase (bla) in plasmids found in Escherichia coli K-12. The aim of this study.. Read More»
Genet. Mol. Res. 15(2): gmr.15028528
DOI: 10.4238/gmr.15028528
Acrocomia emensis (Arecaceae) genetic structure and diversity using SSR molecular markers
Acrocomia emensis, popularly known as the creeping tucum, belongs to the family Arecaceae, and is an oilseed specie of the Brazilian Savannah. The expansion of agricultural activity has rapidly destroyed its natural habitat, leading to a decrease in its population size. Genetic studies can be used to investigate the genetic variability, and may assist with the charting future conservation strat.. Read More»
Genet. Mol. Res. 15(1): gmr.15017785
DOI: 10.4238/gmr.15017785
Characterization of nine transferred SSR markers in the tropical tree species Enterolobium contortisiliquum (Fabaceae)
Transfer of molecular markers is widely used in conservation genetic studies. We investigated the transferability of simple sequence repeat (SSR) markers developed for Enterolobium cyclocarpum to E. contortisiliquum, a tropical tree widely distributed in dry forests. A set of 9 evaluated SSR markers were amplified in E. contortisiliquum and the degree of polymorphism was assessed in 8 trees sam.. Read More»
Genet. Mol. Res. 11(4): 2012.August.17.11
DOI: 10.4238/2012.August.17.11
Antimicrobial activity of fermented Theobroma cacao pod husk extract
Theobroma cacao L. contains more than 500 different chemical compounds some of which have been traditionally used for their antioxidant, anti-carcinogenic, immunomodulatory, vasodilatory, analgesic, and antimicrobial activities. Spontaneous aerobic fermentation of cacao husks yields a crude husk extract (CHE) with antimicrobial activity. CHE was fractioned .. Read More»
Genet. Mol. Res. 13(3): 2014.September.26.10
DOI: 10.4238/2014.September.26.10
Genetic diversity of Annona crassiflora (Annonaceae) in northern Minas Gerais State
Genetic diversity analyses of tropical tree species are relevant to landscape management, plant genetic resource inventory, and biological conservation of threatened species. Annona crassiflora is an endangered fruit tree native to the Cerrado biome that is threatened by reduction of natural populations and fruit extraction. We examined the intra- and interpopulatio.. Read More»
Genet. Mol. Res. 10(3): vol10-3gmr1188
DOI: 10.4238/vol10-3gmr1188
Transferability and characterization of simple sequence repeat markers from Anacardium occidentale to A. humile (Anacardiaceae)
Use of molecular markers can be limited by the high cost and extensive time required for their development. Transfer of simple sequence repeat (SSR) markers reduces the cost and time limitations and has allowed the use of these markers in a larger number of species. We tested 11 SSR markers previously developed for Anacardium occidentale on A. humile. The 11 loci were successfully amplified in .. Read More»
Genet. Mol. Res. 11(4): 2012.October.17.7
DOI: 10.4238/2012.October.17.7
Genetic diversity of Bacillus sp producers of amylase isolated from the soil
The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective of this study was to isolate and analyz.. Read More»
Genet. Mol. Res. 16(3): gmr16039771
DOI: 10.4238/gmr16039771
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