Plant Genetics
Culture-independent analysis of endophytic bacterial communities associated with Brazilian sugarcane
Authors: G.S. Magnani1, L.M. Cruz1, H. Weber2, J.C. Bespalhok2, E. Daros2, V. Baura1, M.G. Yates1, R.A. Monteiro1, H. Faoro1, F.O. Pedrosa1 and E.M. Souza1
Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.3
- DOI:
- 10.4238/2013.October.15.3
QTL identification of ear leaf morphometric traits under different nitrogen regimes in maize
Authors: Z.P. Zheng and X.H. Liu
The ear leaf is one of the most important leaves in maize (Zea mays); it affects plant morphology and yield. To better understand its genetic basis, we examined ear leaf length, ear leaf width, and ear leaf area for quantitative trait locus (QTL) mapping in a recombinant inbred line population under two nitrogen regimes. Nine QTLs, on chromosomes 1 (one),.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.12
- DOI:
- 10.4238/2013.February.28.12
Analysis of mitochondrial DNA using amplified fragment length polymorphism markers of isonuclear allocytoplasmic male sterile wheat accessions and their maintainer lines
Authors: M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu and Z. Xinbo
To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta (T.spelta) -9.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.5
- DOI:
- 10.4238/2013.October.30.5
MicroRNA expression profiling studies on bronchopulmonary dysplasia: a systematic review and meta-analysis
Authors: Y. Yang, J. Qiu, Q. Kan, X.-G. Zhou and X.-Y. Zhou
Over the past several years, several microRNA (miRNA) expression profiling studies have been carried out on bronchopulmonary dysplasia (BPD) in mammalian lung tissues. The most effective way to identify these important miRNAs is to systematically search for similar signatures identified in multiple independent studies. Accordingly, a meta-analysis was con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.4
- DOI:
- 10.4238/2013.October.30.4
Isolation and identification of bovine primary hepatocytes
Authors: Q.D. Jiang1, H.P. Li2, F.J. Liu2, X.J. Wang2, Y.J. Guo2, L.F. Wang2, W.F. Lu2, H.J. Li2, X.P. Li1 and G.Y. Yang2
The liver is a unique organ that is endowed with a plethora of specialized functions. Most of its functional traits are controlled by hepatocytes. Primary hepatocytes have been used widely in in vitro models to understand the biological processes occurring in the liver. There are a number of methods used to separate hepatocytes, but the cell activity and .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.3
- DOI:
- 10.4238/2013.October.30.3
Genetic diversity and relationships among accessions of five crested wheatgrass species (Poaceae: Agropyron) based on gliadin analysis
Authors: S.Y. Chen, X. Ma, X.Q. Zhang, L.K. Huang and J.N. Zhou
Agropyron Gaertn. is the most important genus in Triticeae (Poaceae), which includes many forage grasses with high economic value. The genetic diversity and relationships of 36 accessions from five crested wheatgrass species were analyzed by gliadin markers. A total of 54 product bands were detected after acid polyacrylamide gel electrophoresis (A-PAGE), .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.19
- DOI:
- 10.4238/2013.November.18.19
Incorporation of genetic marker information in beef cattle breeding programs
Authors: F.M. Rezende
The availability of molecular marker information has turned out to be an opportunity to improve animal breeding programs, by the inclusion of those effects in the estimation of breeding values. Along this perspective, the aims of present research were to compare genetic evaluation models that assume or not marker effects in the estimation of breeding valu.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.1
- DOI:
- 10.4238/2013.October.30.1
A high-throughput, high-quality plant genomic DNA extraction protocol
Authors: H. Li1,2, J. Li1,2, X.H. Cong2, Y.B. Duan2,3, L. Li2, P.C. Wei2, X.Z. Lu2 and J.B. Yang2
The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block,.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.1
- DOI:
- 10.4238/2013.October.15.1
Efficient oil palm total RNA extraction with a total RNA extraction kit
Authors: S.H. Habib, H.M. Saud and H. Kausar
Oil palm tissues are rich in polyphenols, polysaccharides and secondary metabolites; these can co-precipitate with RNA, causing problems for downstream applications. We compared two different methods (one conventional and a kit-based method - Easy-BlueTM Total RNA Extraction Kit) to isolate total RNA from leaves, roots and shoot apical meristems of tissue.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.8
- DOI:
- 10.4238/2014.April.3.8
Identification and authentication of Rosa species through development of species-specific SCAR marker(s)
Authors: K.M.I. Bashir, F.S. Awan, I.A. Khan, A.I. Khan and M. Usman
Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the i.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.8