Plant Genetics
A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization
Authors: Y.Q. Zhu1,2, W.J. Wu1,2, H.W. Xiao1,2, H.B. Chen1,2, Y. Zheng1,2, Y.J. Zhang2, H.X.Wang2 and L.Q. Huang1,2
A recently developed revolutionary approach to transcriptomics, RNA-Seq, and suppression subtractive hybridization are powerful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that.. Read More»
- Genet. Mol. Res. 12(4):
Quantative trait loci of seed traits for soybean in multiple environments
Authors: J.Y. Che, J.J. Ding4, C.Y. Liu, D.W. Xin, H.W. Jiang, G.H. Hu and Q.S. Chen
Seed length and seed width are an important factor to the soybean yield. So the quantitative trait loci (QTL) location for seed length and seed width could assistant the breeding of soybean. In this study, the QTL underlying seed length and seed width were studied. A recombinant inbred line population of soybeans derived from .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.11
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.11
Characterization of Capsicum species using anatomical and molecular data
Authors: G.B. Dias, V.M. Gomes, T.M.S. Moraes, U.P. Zottich, G.R. Rabelo, A.O. Carvalho, M. Moulin, L.S.A. Gon�§alves, R. Rodrigues and M. Da Cunha
Capsicum species are frequently described in terms of genetic divergence, considering morphological, agronomic, and molecular databases. However, descriptions of genetic differences based on anatomical characters are rare. We examined the anatomy and the micromorphology of vegetative and reproductive organs of several Capsicum species. Four Capsicum acces.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.29
- DOI:
- 10.4238/2013.February.28.29
Genome-wide analysis of the auxin response factor gene family in cucumber
Authors: S.Q. Liu and L.F. Hu
Auxin response factors (ARFs) participate in the transcriptional regulation of a series of biological processes related to growth and development. The ARF genes comprise a large multigene family in plants. Recently, a draft of the full cucumber (Cucumis sativus) genome assembly has been released; however, none of the ARF genes have been characterized. We .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.April.2.1
- DOI:
- 10.4238/2013.April.2.1
Usefulness of cpDNA markers for phylogenetic and phylogeographic analyses of closely related cactus species
Authors: I.A.S. Bonatelli, D.C. Zappi, N.P. Taylor and E.M. Moraes
Although plastid DNA has been widely explored as a marker of choice for phylogeny and phylogeography studies, little is known about its utility for examining relationships between closely related species. The slow evolutionary rates inherent to chloroplast (cp) DNA make it difficult to perform lower level taxonomic analyses, particularly at the population.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.27
- DOI:
- 10.4238/2013.February.28.27
Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)
Authors: L. Li1, N. Li, S.F. Song, Y.X. Li, X.J. Xia, X.Q. Fu, G.H. Chen and H.F. Deng
The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei’ai 64S in different tissues at different developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated and downregulated genes. I.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.13
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.13
Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear
Authors: J. Tang, J. Lin, B.L. Zhang, Z.H. Wang, X.G. Li and Y.H. Chang
A 1794-bp cDNA fragment was amplified from mRNA isolated from pear (Pyrus pyrifolia NaKai. Cuiguan) leaves by using primers based on the sequences generated during the analysis of the pear transcriptome. The 597-amino acid sequence encoded by the cDNA was compared with the sequences in GenBank, and it was found to be similar to that of members of the sucr.. Read More»
- Genet. Mol. Res. 13(4):
Different timing and spatial separation of parental chromosomes in intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus
Authors: L. Ding, Z.G. Zhao, X.H. Ge and Z.Y. Li
Experimental and newly formed hybrids and polyploids generated by wide crosses usually show varying degrees of cytological instability. The spatial separation of parental genomes and uniparental chromosome elimination in hybrid cells has been reported in many hybrids from plants and animals. Herein, the behavior of parental ge.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.3
- DOI:
- 10.4238/2014.April.8.3
Vasoactive intestinal polypeptide suppresses proliferation of human cord blood-derived hematopoietic progenitor cells by increasing TNF-�± and TGF-�² production in the liver
Authors: L. Wang1, Q. Xiao1, C.H. Wang3, X. Li3, S.Q. Luo2 and C.W. Tang3
The physiology of hepatic hematopoiesis is largely unknown, although studies have indicated that vasoactive intestinal polypeptide (VIP) is involved in this disease. To validate this hypothesis, we assessed the effects of VIP on human cord blood CD34+ cells. We also measured VIP levels and the capacity of vasoactive intestinal polypeptide receptor (VIPR) .. Read More»
- Genet. Mol. Res. 13(4):
Use of morpho-agronomic traits and DNA profiling for classification of genetic diversity in papaya
Authors: O.N. de Jesus, J.P.X. de Freitas, J.L.L. Dantas and E.J. de Oliveira
We examined the genetic diversity of papaya (Carica papaya) based on morpho-agronomic and molecular data. Twenty-seven genotypes grown in Brazil were analyzed with 11 AFLP primer combinations, 23 ISSR markers, 22 qualitative, and 30 quantitative descriptors. For the joint analyses, we used the Gower algorithm (Joint Gower) and the average value of the ind.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.July.11.8
- DOI:
- 10.4238/2013.July.11.8