Human Genetics
Evolution without speciation but with selection: LUCA, the Last Universal Common Ancestor in Gilbertâ??s RNA world
Authors: Hugo Hoenigsberg
This is not an attempt to analyze the Last Universal Common Ancestor (LUCA) to understand the origin of living systems. We do not know what came before Gilberts’ RNA world. Our analysis starts with the RNA world and with genes (biological replicators alla Dawkings) made up of RNA proteins with enzymatic catalytic functio.. Read More»
- Genet. Mol. Res. 2(4):
Evaluation of the mutagenic activity of hydroxyurea on the G1-S-G2 phases of the cell cycle: an in vitro study
Authors: Patr?cia Danielle Lima de Lima, Pl?nio Cerqueira dos Santos Cardoso, Andr?© Salim Khayat, Marcelo de Oliveira Bahia, Rommel Rodr?guez Burbano
Hydroxyurea is considered an antineoplastic drug, which also plays an important role in the treatment of sickle cell anemia patients. We evaluated and compared the clastogenic and cytotoxic effects of hydroxyurea, using chromosomal aberrations and mitotic index, respectively, as endpoints. In vitro short-term cultures of lymph.. Read More»
- Genet. Mol. Res. 2(3):
Genetic, biochemical, and anatomic characterization of neurological mutant 3, a new mouse model for Parkinsonâ??s disease
Authors: Marie E. Legare, William H. Hanneman
We have identified a new mutant mouse that we have named new mouse neurological mutant 3 (NM3); it may be a useful model to understand the underlying molecular and genetic basis of Parkinson’s disease (PD). A mouse carrying the NM3 mutation arose spontaneously in an RIIIS/J breeding colony and was identified as having a .. Read More»
- Genet. Mol. Res. 2(3):
Psorophora columbiae and Psorophora toltecum (Diptera: Culicidae) Colombian populations cannot be differentiated by isoenzymes
Authors: Manuel Ruiz-Garcia, Diana Ramirez, Felio Bello, Diana Alvarez
Two populations of the mosquito Psorophora columbiae from the central Andean area of Colombia and one population of Ps. toltecum from the Atlantic coast of Colombia were analyzed for 11 isoenzyme markers. Psorophora columbiae and Ps. toltecum are two of the main vectors of Venezuelan equine encephalitis. We found no conspicuou.. Read More»
- Genet. Mol. Res. 2(2):
The anticancer homeopathic composite â??Canova Methodâ? is not genotoxic for human lymphocytes in vitro
Authors: Igor C. Seligmann, Patr?cia D.L. Lima, Pl?nio C.S. Cardoso, Andr?© S. Khayat, Marcelo O. Bahia, Dorli de Freitas Buchi Isabel R. Cabral, Rommel R. Burbano
The Canova Methodâ (CM) is a homeopathic medicine indicated for the treatment of patients with cancer and for pathologies that involve a depressed immune system, such as AIDS. This product is composed of homeopathic dilutions of Aconitum napellus, Arsenicum album (arsenic trioxide), Bryonia alba, Lachesis muta venom and .. Read More»
- Genet. Mol. Res. 2(2):
Birth of normal kids after microinjection of pronuclear embryos in a transgenic goat (Capra hircus) production program in Brazil
Authors: V.J.F. Freitas, I.A. Serova, L.E. Andreeva, E.S. Lopes J?ºnior, D.I.A. Teixeira, M.F. Cordeiro, D. Rondina, N.R.O. Paula, I.J. Arruda, J.B. Lima Verde, G. Dvoriantchikov, O. Serov
This pilot project was designed to determine if normal kids could be produced after microinjection in pronuclear embryos and subsequent transfer to recipients in a transgenic goat program in Brazil. Twelve donors of the Saanen breed and 17 recipients of an undefined breed were used. The estrus of both donors and recipients was.. Read More»
- Genet. Mol. Res. 2(2):
Anti-P53 antibodies in Brazilian brain tumor patients
Authors: Renata Fragelli Fonseca, M?¡rcia Tie Kawamura, Jos?© Ant?´nio Oliveira, Anselmo Teixeira, Gilda Alves, Maria da Gl?³ria da Costa Carvalho
Gliomas of astrocytic origin are the most common primary brain tumors, accounting for over 40 to 50% of all central nervous system tumors. The TP53 tumor suppressor gene is the most frequently mutated gene found in human malignancies. A mutation of this gene can lead to an increased half-life of the resulting protein and loss .. Read More»
- Genet. Mol. Res. 2(2):
Evaluation of a method for high yield purification of largely intact mitochondrial DNA from human placentae
Authors: Sergio U. Dani, Alessandra Cristina Gomes-Ruiz, Maria Angela C. Dani
We developed, and quantitatively and qualitatively evaluated an easily reproducible method for high yield purification of mitochondrial DNA (mtDNA) from human placentae by mechanical tissue disruption, differential centrifugation of mitochondria, enzymatic digestion, phenol extraction and ethanol precipitation. Average mtDNA y.. Read More»
- Genet. Mol. Res. 2(2):
Molecular epidemiology of the hepatitis C virus in Brazil
Authors: Solange Busek, Guilherme Oliveira
Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The genome of this virus consists of approximately 10,000 bp and codes for 10 mature polypeptides. Genome sequence comparison has revealed the existence of six major genotypes and a large number of subtypes. The genotypes can be distinguished by wh.. Read More»
- Genet. Mol. Res. 2(1):
GAL4 causes developmental defects and apoptosis when expressed in the developing eye of Drosophila melanogaster
Authors: Jamie M. Kramer, Brian E. Staveley
The UAS/GAL4 ectopic expression system is widely used in Drosophila melanogaster for the overexpression of transgenes. This system operates under the assumption that the yeast transcription factor, GAL4, is inactive in D. melanogaster. Thus, GAL4 can be expressed under the control of D. melanogaster-specific promoters with lit.. Read More»
- Genet. Mol. Res. 2(1):