Human Genetics
Evaluation of window cohabitation of DNA sequencing errors and lowest PHRED quality values
Authors: Francisco Prosdocimi, Fabiano Cruz Peixoto and Jos�© Miguel Ortega
When analyzing sequencing reads, it is important to distinguish between putative correct and wrong bases. An open question is how a PHRED quality value is capable of identifying the miscalled bases and if there is a quality cutoff that allows mapping of most errors. Considering the fact that a low quality value does not necess.. Read More»
- Genet. Mol. Res. 3(4):
VSQual: a visual system to assist DNA sequencing quality control
Authors: Eliseu Binneck, Jo�£o Fl�¡vio V. Silva, Norman Neumaier,Jos�© Renato B. Farias and Alexandre L. Nepomuceno
A lack of pliant software tools that support small- to medium-scale DNA sequencing efforts is a major hindrance for recording and using laboratory workflow information to monitor the overall quality of data production. Here we describe VSQual, a set of Perl programs intended to provide simple and powerful tools to check severa.. Read More»
- Genet. Mol. Res. 3(4):
Comparative analysis of noncoding sequences of orthologous bovine and human gene pairs
Authors: Melissa Nunes Miziara, Penny K. Riggs and M. Elisabete J. Amaral
Genomic sequence comparison across species has enabled the elucidation of important coding and regulatory sequences encoded within DNA. Of particular interest are the noncoding regulatory sequences, which influence gene transcriptional and posttranscriptional processes. A phylogenetic footprinting strategy was employed to iden.. Read More»
- Genet. Mol. Res. 3(4):
Molecular characterization of globin chain mutants (CaracterizaÃ?§Ã?£o molecular de mutantes de cadeias globÃ?Ânicas)
Authors: A.R. Chinelato-Fernandes
-.. Read More»
- Genet. Mol. Res. 3(3):
Reduction of non-digestible oligosaccharides in soy milk: application of engineered lactic acid bacteria that produce �±-galactosidase
Authors: Jean Guy LeBlanc, Aurelio Silvestroni, Cristelle Connes,Vincent Juillard, Graciela Savoy de Giori, Jean-Christophe Piard and Fernando Sesma
Human consumption of soy-derived products has been limited by the presence of non-digestible oligosaccharides (NDO), such as the a-galactooligosaccharides raffinose and stachyose. Most mammals, including man, lack pancreatic a-galactosidase (a-Gal), which is necessary for the hydrolysis of these sugars. However, such NDO can b.. Read More»
- Genet. Mol. Res. 3(3):
Oncogene-induced transcriptional patterns in established cell lines as a model for in vitro analysis of tumor biology
Authors: Francisco de Paula Careta, Bruno Marcos Verbeno Azevedo,Beatriz Alves Vianna1, Greiciane Gaburro Paneto,Irineu Iester Degasperi, Marcos do Carmo Oyama,Val�©ria Fagundes, ��ngela Maria Spagnol Perrone andI�ºri Drumond Louro
Established cell lines have long been used for in vitro studies of tumor biology, enabling investigators to control growth conditions and to draw important conclusions about the oncogenic microenvironment. However, gene expression behavior in cultured cells may not always reflect the actual in vivo scenario, and analysis deriv.. Read More»
- Genet. Mol. Res. 3(3):
Less Ã?Â?mtDNA4977 than normal in various types of tumors suggests that cancer cells are essentially free of this mutation
Authors: Maria Angela C. Dani, Sergio U. Dani, Silmara P.G. Lima, Alfredo Martinez, Benedito M. Rossi, Fernando Soares, Marco A. Zago and Andrew J.G. Simpson
Levels of mtDNA4977 deletions (DmtDNA4977) have been found to be lower in tumors than in adjacent non-tumoral tissues. In 87 cancer patients, DmtDNA4977 was detected by multiplex polymerase chain reaction (PCR) amplification in 43 (49%) of the tumors and in 74 (85%) of the samples of non-tumoral tissues that were adjacent to t.. Read More»
- Genet. Mol. Res. 3(3):
Heritability estimates for carcass traits of cattle: a review
Authors: Angel RÃ?Âos Utrera and Lloyd Dale Van Vleck
We present estimates of heritability for carcass traits of cattle published in the scientific literature. Seventy-two papers published from 1962 to 2004, which reported estimates of heritability for carcass traits, were reviewed. The unweighted means of estimates of heritability for 14 carcass traits by slaughter end point (ag.. Read More»
- Genet. Mol. Res. 3(3):
Cytogenetics of genetic counseling patients in Pelotas, Rio Grande do Sul, Brazil
Authors: A.C. Duarte1, E. Cunha, J.M. Roth, F.L.S. Ferreira, G.L. Garcias and M.G. Martino-Roth
From 1986 to 2002, we examined the chromosomal composition of 916 patients attended by two genetic counseling services in the city of Pelotas, in the Brazilian State of Rio Grande do Sul, to determine the genetic causes of their disturbances. Patterns of G-banding using trypsin and Giemsa (GTG) and C-banding using barium and G.. Read More»
- Genet. Mol. Res. 3(3):
Microcompetition with Foreign DNA and the Origin of Chronic Disease
Authors: Hanan Polansky
-.. Read More»
- Genet. Mol. Res. 3(2):