Bioinformatics
Bioinformatics of the Paracoccidioides brasiliensis EST Project
Authors: Marcelo M. BrÃ?Âgido, Maria EmÃ?Âlia M.T. Walter, Adilton G. Oliveira, Marcus K. Inoue, Daniel S. Anjos, Edans F.O. Sandes, JoÃ?£o J. Gondim, Maria JosÃ?© de A. Carvalho, Nalvo F. Almeida Jr. and Maria Sueli Soares Felipe
Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an endemic mycosis of Latin America. This fungus presents a dimorphic character; it grows as a mycelium at room temperature, but it is isolated as yeast from infected individuals. It is believed that the transition from mycelium to yeast is impor.. Read More»
- Genet. Mol. Res. 4(2):
B-type natriuretic peptide and cirrhosis progression
Authors: L.Y. Shi, R. Jin, C.J. Lin, J.S. Wu, X.W. Chen, Z. Yu and P.C. Zhang
Brain natriuretic peptide (BNP) is used as a marker of cardiac dysfunction to predict heart failure mortality. The significance of the prognostic ability of BNP for liver cirrhosis remains unknown, although the levels of BNP seen in cirrhosis are high. We aimed to determine whether the BNP level is related to the stage of cirrhosis and could serve as a pr.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.9
- DOI:
- 10.4238/2015.May.18.9
Fyn arrests swainsonine-induced apoptosis in 293T cells via Akt and its phosphorylation
Authors: L. An, W.W. Li and G.C. Cheng
Swainsonine (SW), an extract from Astragalus membranaceus, represents a new class of compounds that inhibit growth and induce apoptosis in a cancer model. In this study, we demonstrated the effect of Fyn on SW-induced apoptosis in 293T cells. Western blotting was used to measure the expression of the apoptosis-related factors caspase-3, Bcl-2, Bax, and th.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.23
- DOI:
- 10.4238/2015.May.18.23
Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers
Authors: Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers
Thyroid hormones play an important role in regulating metabolism and can affect metabolism-related traits such as fat deposition. The thyroglobulin (TG) gene produces the precursor of thyroid hormones and has been proposed as a candidate gene for a quantitative trait locus with an effect on fat deposition. In this study, we identified 4 novel single nucle.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.2
- DOI:
- 10.4238/2015.May.22.2
Effects of Thai black sticky rice extract on oxidative stress and lipid metabolism gene expression in HepG2 cells
Authors: W. Sangkitikomol, T. Tencomnao and A. Rocejanasaroj
Anthocyanins, which are found in some food, including Thai black sticky rice, are reported to have health-promoting properties. Oxidative stress plays a major role in the pathogenesis of many degenerative diseases induced by free radicals, such as cardiovascular disease, stroke and cancer. We evaluated the anthocyanin-rich ext.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr912
- DOI:
- 10.4238/vol9-4gmr912
Genetic relationships between selected Turkish mulberry genotypes (Morus spp) based on RAPD markers
Authors: E. Orhan and S. Ercisli
Mulberry (Morus spp, Moraceae) is an important horticultural crop in Turkey, which is one of the main world producers of mulberry fruit. We evaluated the genetic relationships among 26 mulberry genotypes selected for agronomic characteristics, using RAPD markers. A total of 367 DNA markers were generated with 34 random primers.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr958
- DOI:
- 10.4238/vol9-4gmr958
Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora
Authors: M.M. Souza, J.D. Urdampilleta and E.R. Forni-Martins
Cytological preparations for the fluorescent in situ hybridization (FISH) technique require cytoplasm-free metaphases, with well-spread chromosomes, for the localization of DNA sequences and chromosome mapping. We tested various procedures for FISH analysis of Passiflora cacaoensis, P. gardneri and hybrid F1 progeny of P. gard.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr951
- DOI:
- 10.4238/vol9-4gmr951
Molecular and functional analysis of the poly-�²-hydroxybutyrate biosynthesis operon of Pseudomonas sp BJ-1
Authors: S.W. Zhu, Z.Y. Fang, H.Y. Jiang and B.J. Cheng
The operon comprising the genes for poly-β-hydroxybutyrate (PHB) biosynthesis in Pseudomonas sp BJ-1 was cloned and sequenced. Sequence analysis of 8991 bp revealed that the regions contain two related operons. The first operon contains the three genes phbA, phbB and phbC, and the other contains the two genes flp1 and flp.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr845
- DOI:
- 10.4238/vol9-4gmr845
Citrullinemia type I: molecular screening of the ASS1 gene by exonic sequencing and targeted mutation analysis
Authors: R. Marquis-Nicholson, E. Glamuzina, D. Prosser, C. Wilson and D.R. Love
We developed a mutation-screening protocol for the ASS1 gene in order to guide clinical management of neonates with elevated citrulline detected during routine newborn screening. An exon-based amplification and sequencing method was designed and successfully applied to patients to identify disease-associated mutations. The seq.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr834
- DOI:
- 10.4238/vol9-3gmr834
Combining P values to improve classification of differential gene expression in the HTself software
Authors: D.A. Cortez, A.P. Tonon, P. Colepicolo and R.Z.N. V�ªncio
HTself is a web-based bioinformatics tool designed to deal with the classification of differential gene expression in low replication microarray studies. It is based on a statistical test that uses self-self experiments to derive intensity-dependent cutoffs. We developed an extension of HTself, originally released in 2005, by .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.5.5
- DOI:
- 10.4238/2011.December.5.5