Animal Genetics
A picture of gene sampling/expression in model organisms using ESTs and KOG proteins
Authors: MaurÃ?Âcio de Alvarenga Mudado and JosÃ?© Miguel Ortega
The expressed sequence tag (EST) is an instrument of gene discovery. When available in large numbers, ESTs may be used to estimate gene expression. We analyzed gene expression by EST sampling, using the KOG database, which includes 24,154 proteins from Arabidopsis thaliana (Ath), 17,101 from Caenorhabditis elegans (Cel), 10,51.. Read More»
- Genet. Mol. Res. 5(1):
cDNA, genomic sequence cloning and overexpression of ribosomal protein gene L9 (rpL9) of the giant panda (Ailuropoda melanoleuca)
Authors: W.R. Hou, Y.L. Hou, G.F. Wu, Y. Song, X.L. Su, B. Sun and J. Li
The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mam.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1159
- DOI:
- 10.4238/vol10-3gmr1159
In silico prediction of yeast deletion phenotypes
Authors: Soma Saha and Steffen Heber
Analysis of gene deletions is a fundamental approach for investigating gene function. We evaluated an algorithm that uses classification techniques to predict the phenotypic effects of gene deletions in yeast. We used a modified simulated annealing algorithm for feature selection and weighting. The selected features with high .. Read More»
- Genet. Mol. Res. 5(1):
Karyotype of Brazilian Anopheles albitarsis sensu lato (Diptera:Culicidae)
Authors: MÃ?Âriam Silva Rafael, Ivanildo Pereira dos Santos-Junior, Wanderli Pedro Tadei, Maria Anice Mureb Sallum and Oswaldo Paulo Forattini
Anopheles (Nyssorhynchus) albitarsis sensu lato is an important malaria vector in Brazil, especially in the Brazilian Amazon region. Chromosome preparations of fourth-instar larvae of A. albitarsis from Iranduba and Coari (AM) and Ilha Comprida (SP) were analyzed for karyotype determination and to improve cytogenetic identific.. Read More»
- Genet. Mol. Res. 4(4):
Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection
Authors: Mariela Nieves, MarÃ?Âa MÃ?¼hlmann and Marta Dolores Mudry
Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome microdissection to develop a.. Read More»
- Genet. Mol. Res. 4(4):
Molecular phylogeny of penaeid shrimps inferred from two mitochondrial markers
Authors: Carolina M. Voloch, Pablo R. Freire and Claudia A.M. Russo
Penaeid shrimps are an important resource in crustacean fisheries, representing more than the half of the gross production of shrimp worldwide. In the present study, we used a sample of wideranging diversity (41 shrimp species) and two mitochondrial markers (758 bp) to clarify the evolutionary relationships among Penaeidae gen.. Read More»
- Genet. Mol. Res. 4(4):
Karyotypic characterization of Iheringichthys labrosus (Pisces, Pimelodidae): C-, G- and restriction endonuclease banding
Authors: Rafael Augusto de Carvalho and Ana L�ºcia Dias
Various chromosomal banding techniques were utilized on the catfish, Iheringichthys labrosus, taken from the Capivara Reservoir. C-banding regions were evidenced in telomeric regions of most of the chromosomes. The B microchromosome appeared totally heterochromatic. The restriction endonuclease AluI produced a banding pattern .. Read More»
- Genet. Mol. Res. 4(4):
Characters that differ between diploid and haploid honey bee (Apis mellifera) drones
Authors: Matthias Herrmann, Tina Trenzcek, Hartmut Fahrenhorst and Wolf Engels
Diploid males have long been considered a curiosity contradictory to the haplo-diploid mode of sex determination in the Hymenoptera. In Apis mellifera, ‘false’ diploid male larvae are eliminated by worker cannibalism immediately after hatching. A ‘cannibalism substance’ produced by diploid drone larvae .. Read More»
- Genet. Mol. Res. 4(4):
Hydrolases in the hypopharyngeal glands of workers of Scaptotrigona postica and Apis mellifera (Hymenoptera, Apinae)
Authors: Rosil�©ia A.C. Costa and Carminda da Cruz-Landim
Hydrolytic enzymes from hypopharyngeal gland extracts of newly emerged, nurse and foraging workers of two eusocial bees, Scaptotrigona postica, a native Brazilian stingless bee, and the Africanized honey bee (Apis mellifera) in Brazil, were compared. The hypopharyngeal gland is rich in enzymes in both species. Fifteen differen.. Read More»
- Genet. Mol. Res. 4(4):
Genetic trends of abdominal fat content in a male broiler chicken line
Authors: Leila de Genova Gaya, Gerson Barreto MourÃ?£o, Fernanda Marcondes de Rezende, ElisÃ?¢ngela Chicaroni de Mattos, TÃ?©rcio Michelan Filho, LuÃ?Âs Gustavo Girardi Figueiredo, JosÃ?© Bento Sterman Ferraz1 and Joanir Pereira Eler
Data of chickens from a broiler-breeding program were collected and used to determine the genetic trends of absolute and relative abdominal fat content. The genetic trends were estimated by the regression of trait genetic value averages on hatch-years. Genetic values from 32,485 individuals were used for regression analysis. T.. Read More»
- Genet. Mol. Res. 4(4):