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Animal Genetics

Animal Genetics   Research Article

First chromosome data on Steindachneridion scripta (Pisces, Siluriformes, Pimelodidae) from Brazilian rivers: Giemsa, CBG, G-, and RE banding

Authors: Ana Claudia SwarĂ?§a, Alberto Sergio Fenocchio, Marta Margarete Cestari and Ana LĂ?Âșcia Dias

A cytogenetic study was performed on the large pimelodid species Steindachneridion scripta (Siluriformes, Sorubiminae) from the Paraná River basin (Brazil). Chromosome preparations were obtained avoiding sacrifice of the specimens, by means of lymphocyte culture, and several staining and chromosome banding techniques we.. Read More»

Genet. Mol. Res. 4(4):
Animal Genetics   Research Article

Applicability of RAPD markers on silverstained polyacrylamide gels to ascertain genetic diversity in Peripatus acacioi (Peripatidae; Onychophora)

Authors: Daiane Mariele DeLaat, Maria Raquel Santos Carvalho, Maria Bernadete Lovato, Maria Dolores Porto Acedo and Cleusa Gra�§a da Fonseca

RAPD (random amplification of polymorphic DNA) molecular markers can be utilized for analyzing genetic variability in populations for which only a few or no molecular markers are available. They were used in a study of an endangered species, Peripatus acacioi, found in the Tripuí Ecological Station, in Ouro Preto, MG, B.. Read More»

Genet. Mol. Res. 4(4):
Animal Genetics   Research Article

Development and optimization of a fluorescent differential display PCR system for studying bovine embryo development in vitro

Authors: Paula Ripamonte, Giovana Krempel Merighe, Yeda Fumie Watanabe, Alexandre Rodrigues Caetano and FlĂ?ÂĄvio Vieira Meirelles

Differential display is a widely used methodology to identify genes that are differentially expressed in biological samples. We developed a new protocol for the amplification and recovery of differentially expressed genes from extremely small initial amounts of RNA (10 to 25 pg mRNA) from preimplantation bovine embryos. The cD.. Read More»

Genet. Mol. Res. 4(4):
Animal Genetics   Research Article

Single nucleotide polymorphism analysis of exons 3 and 4 of IGF-1 gene in pigs

Authors: L.L. Hao, H. Yu, Y. Zhang, S.C. Sun, S.C. Liu, Y.Z. Zeng, Y.X. Ai and H.Z. Jiang

The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were .. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1328
DOI:
10.4238/vol10-3gmr1328
Animal Genetics   Research Article

Breeding cassava for apomixis

Authors: Nagib M.A. Nassar and Rosane G. Collevatti

Apomixis genes have been successfully transferred to cassava (Manihot esculenta) by hybridizing it with the wild species, M. glaziovii. The interspecific hybrid of cassava and M. glaziovii was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were g.. Read More»

Genet. Mol. Res. 4(4):
Animal Genetics   Short communication

Isolation and characterization of 19 polymorphic microsatellite loci from the topmouth gudgeon, Pseudorasbora parva

Authors: C. Zeng, Y. Gul, K. Yang, L. Cui, W.-M. Wang and Z.-X. Gao

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ez.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1477
DOI:
10.4238/vol10-3gmr1477
Animal Genetics   Research Article

Localization of rDNA sites in holocentric chromosomes of three species of triatomines (Heteroptera, Triatominae)

Authors: Grasiela Dias de Campos Severi-Aguiar and Maria Terc�­lia Vilela de Azeredo-Oliveira

Chromatin organization in the holocentric chromosomes of three triatomines species was cytologically studied by fluorescent in situ hybridization with a 45S rDNA probe of Drosophila melanogaster to localize ribosomal genes. In Triatoma tibiamaculata, metaphases I showed telomeric highlights in a single, larger bivalent. In T. .. Read More»

Genet. Mol. Res. 4(4):
Animal Genetics   Research Article

Development and characterization of microsatellite markers for the lizardfish known as the Bombay duck, Harpadon nehereus (Synodontidae)

Authors: T.J. Xu, D.Q. Sun, H.Y. Li and R.X. Wang

The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1045
DOI:
10.4238/vol10-3gmr1045
Animal Genetics   Research Article

Allele frequencies and genetic diversity in two groups of wild tufted capuchin monkeys (Cebus apella nigritus) living in an urban forest fragment

Authors: Jeanne Margareth Jimenes Amaral, Aguinaldo Luiz Sim�”es and David De Jong

There have been numerous studies genetically characterizing Old World Primates using microsatellites. However, few studies have been made of New World species and none on free-ranging Cebus apella, even though it is probably the most widely distributed species of monkey in the New World. The paucity of studies is due, in part,.. Read More»

Genet. Mol. Res. 4(4):
Animal Genetics   Research Article

Isolation of transfected fibroblast clones for use in nuclear transfer and transgene detection in cattle embryos

Authors: Eduardo O. Melo, Regivaldo V. Sousa, L�­lian T. Iguma, Maur�­cio M. Franco, Elibio L. Rech and Rodolfo Rumpf

Transgenesis in cattle has provided numerous opportunities for livestock production. The development of nuclear transfer (NT) technology has improved the production of transgenic livestock. However, the isolation of pure colonies from a single transfection event remains laborious and can be a constraint in the production of tr.. Read More»

Genet. Mol. Res. 4(4):