Authors: Ana Claudia SwarĂ?§a, Alberto Sergio Fenocchio, Marta Margarete Cestari and Ana LĂ?Âșcia Dias
A cytogenetic study was performed on the large pimelodid species Steindachneridion scripta (Siluriformes, Sorubiminae) from the Paraná River basin (Brazil). Chromosome preparations were obtained avoiding sacrifice of the specimens, by means of lymphocyte culture, and several staining and chromosome banding techniques we.. Read More»
Authors: Daiane Mariele DeLaat, Maria Raquel Santos Carvalho, Maria Bernadete Lovato, Maria Dolores Porto Acedo and Cleusa Gra�§a da Fonseca
RAPD (random amplification of polymorphic DNA) molecular markers can be utilized for analyzing genetic variability in populations for which only a few or no molecular markers are available. They were used in a study of an endangered species, Peripatus acacioi, found in the Tripuí Ecological Station, in Ouro Preto, MG, B.. Read More»
Authors: Paula Ripamonte, Giovana Krempel Merighe, Yeda Fumie Watanabe, Alexandre Rodrigues Caetano and FlĂ?ÂĄvio Vieira Meirelles
Differential display is a widely used methodology to identify genes that are differentially expressed in biological samples. We developed a new protocol for the amplification and recovery of differentially expressed genes from extremely small initial amounts of RNA (10 to 25 pg mRNA) from preimplantation bovine embryos. The cD.. Read More»
Authors: L.L. Hao, H. Yu, Y. Zhang, S.C. Sun, S.C. Liu, Y.Z. Zeng, Y.X. Ai and H.Z. Jiang
The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were .. Read More»
Authors: Nagib M.A. Nassar and Rosane G. Collevatti
Apomixis genes have been successfully transferred to cassava (Manihot esculenta) by hybridizing it with the wild species, M. glaziovii. The interspecific hybrid of cassava and M. glaziovii was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were g.. Read More»
Authors: C. Zeng, Y. Gul, K. Yang, L. Cui, W.-M. Wang and Z.-X. Gao
The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ez.. Read More»
Authors: Grasiela Dias de Campos Severi-Aguiar and Maria TercĂ?Âlia Vilela de Azeredo-Oliveira
Chromatin organization in the holocentric chromosomes of three triatomines species was cytologically studied by fluorescent in situ hybridization with a 45S rDNA probe of Drosophila melanogaster to localize ribosomal genes. In Triatoma tibiamaculata, metaphases I showed telomeric highlights in a single, larger bivalent. In T. .. Read More»
Authors: T.J. Xu, D.Q. Sun, H.Y. Li and R.X. Wang
The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety.. Read More»
Authors: Jeanne Margareth Jimenes Amaral, Aguinaldo Luiz Sim�”es and David De Jong
There have been numerous studies genetically characterizing Old World Primates using microsatellites. However, few studies have been made of New World species and none on free-ranging Cebus apella, even though it is probably the most widely distributed species of monkey in the New World. The paucity of studies is due, in part,.. Read More»
Authors: Eduardo O. Melo, Regivaldo V. Sousa, LĂ?Âlian T. Iguma, MaurĂ?Âcio M. Franco, Elibio L. Rech and Rodolfo Rumpf
Transgenesis in cattle has provided numerous opportunities for livestock production. The development of nuclear transfer (NT) technology has improved the production of transgenic livestock. However, the isolation of pure colonies from a single transfection event remains laborious and can be a constraint in the production of tr.. Read More»