Se
shRNA inhibits the expression of chicken telomerase reverse transcriptase in MDCC-MSB1 cells
Authors: N. Jiang, X.B. Zheng, Z.Y. Zhao, Z.G. Qin and T.J. Liu
Here, we investigated the effects of blocking chicken telomerase reverse transcriptase (chTERT) in MDCC-MSB1 cells, using small-hairpin RNAs (shRNAs) to interfere with gene expression. shRNAs specific to chTERT mRNA were designed, cloned into DNA plasmid vectors, and transfected into MDCC-MSB1 cells. The transfected chTERT RNAs were expressed by the RNA p.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016979
- DOI:
- 10.4238/gmr.15016979
Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity
Authors: F.C. Santos, F. Caixeta, A.C.S. Clemente, E.V. Pinho and S.D.V.F. Rosa
Seeds collected at different maturation stages vary in physiological quality and patterns of protective antioxidant systems against deterioration. In this study we investigated the expression of genes that codify catalase (CAT), dismutase superoxide (SOD), and polyphenol oxidase (PPO) during the pre- and post-physiological maturation phases in whole seeds.. Read More»
- Genet. Mol. Res. 13(4):
- 2014
- DOI:
- 10.4238/2014
Identification of PML/RAR�± fusion gene transcripts that showed no t(15;17) with conventional karyotyping and fluorescent in situ hybridization
Authors: A. Choughule S. Polampalli P. Amre S. Shinde S. Banavali K. Prabhash R. Nair P.G. Subramanian S. Gujral P.M. Parikh
Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17)(q22;q11-21), resulting in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARα) genes. Using conventional cytogenetic methods, these translocations are normally detected in about 70-90% of patients.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr488
- DOI:
- 10.4238/vol8-1gmr488
Candida albicans GRX2, encoding a putative glutaredoxin, is required for virulence in a murine model
Authors: Chaves GM, Bates S, MacCallum DM and Odds FC
Resistance of Candida albicans to reactive oxygen species is thought to enhance its virulence in mammalian hosts. Genes such as SOD1, which encodes the anti-oxidant, superoxide dismutase, are known virulence factors. We disrupted the gene GRX2, which encodes a putative glutathione reductase (glutaredoxin) in C. albicans, and w.. Read More»
- Genet. Mol. Res. 6(4):
Influence of interleukin-1�² and interleukin-6 gene polymorphisms on the development of acute pancreatitis
Authors: D.Z. Chi, J. Chen and D.P. Huang
We investigated the association between 3 main proinflammatory cytokines [interleukin (IL)-1b and IL-6] and the risk of acute pancreatitis. Polymerase chain reaction-restriction fragment length polymorphism was used to genotype IL-1Ã?² +3954 C/T (rs1143634) and IL-1Ã?² -511 C/T (rs16944) and IL-6 -174 G/C (rs1800795) and IL-6 -634 C/G (rs1800796). The geno.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.3.5
- DOI:
- 10.4238/2015.February.3.5
Polymorphisms and DNA methylation of gene TP53 associated with extra-axial brain tumors
Authors: L.O. Almeida A.C. Cust�³dio G.R. Pinto M.J. Santos J.R.W. Almeida C.A. Clara J.A. Rey C. Casartelli
The p53 tumor suppressor gene is the most frequently mutated gene in human cancer; this gene is mutated in up to 50% of human tumors. It has a critical role in the cell cycle, apoptosis and cell senescence, and it participates in many crucial physiological and pathological processes. Polymorphisms of p53 have been suggested to.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr518
- DOI:
- 10.4238/vol8-1gmr518
Effects of VEGF suppression by small hairpin RNA interference combined with radiotherapy on the growth of cervical cancer
Authors: L. Qi, L.N. Xing, X. Wei and S.G. Song
This study aimed to investigate the inhibition of vascular endothelial growth factor (VEGF) by small hairpin RNA (shRNA) interference combined with radiotherapy on the growth of cervical cancer SiHa cell xenografts in nude mice. The effective pVEGF-shRNA plasmid was screened by reverse transcription-polymerase chain reaction (.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.2
- DOI:
- 10.4238/2014.July.7.2
Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)
Authors: X. Chen Y.Q. Su J. Wang M. Liu S.F. Niu S.P. Zhong F. Qiu
In order to investigate the immune role of ribosomal protein L10 (RPL10/QM-like gene) in marine fish, we challenged the large yellow croaker Pseudosciaena (= Larimichthys) crocea, the most important marine fish culture species in China, by injection with a mixture of the bacteria Vibrio harveyi and V. parahaemolyticus (3:1 in volume). Microarray analysis .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.15.7
- DOI:
- 10.4238/2012.October.15.7
Analysis of thyroid peroxidase antibody in early pregnancy
Authors: L. Lin, X.L. Zhang and Y. Long
This study aimed to investigate the thyroid peroxidase antibody (TPOAb) positive rate and how TPOAb affects thyroid function between 7 and 12 weeks of gestation. Six hundred and eleven cases of healthy primipara were enrolled in this study between September 2010 and June 2011, and all of them were given a regular prenatal chec.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.3
- DOI:
- 10.4238/2014.July.7.3
Transcriptome analysis and anthocyanin-related genes in red leaf lettuce
Authors: Y.Z. Zhang*, S.Z. Xu*, Y.W. Cheng, H.Y. Ya and J.M. Han
This study aimed to analyze the transcriptome profile of red lettuce and identify the genes involved in anthocyanin accumulation. Red leaf lettuce is a popular vegetable and popular due to its high anthocyanin content. However, there is limited information available about the genes involved in anthocyanin biosynthesis in this species. In this study, trans.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017023
- DOI:
- 10.4238/gmr.15017023