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Allele frequencies of microsatellite loci for genetic characterization of a Sicilian bovine population
Authors: M. Cosenza, S. Reale, T. Lupo, F. Vitale and S. Caracappa
Short tandem repeats are used as an effective method to trace DNA markers in genotyping. Using a standardized kit, we tested 11 microsatellite markers recommended by the International Society for Animal Genetics (ISAG) in a sample of 495 Sicilian cattle. The aim of this study was to investigate the allele frequencies in the Sicilian cattle population to prov.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.12
- DOI:
- 10.4238/2015.January.30.12
Molecular authentication of Gynostemma pentaphyllum through development and application of random amplification polymorphic DNA sequence-characterized amplified region marker
Authors: J. Zhou,Y.S. Wu, R.Q. Zhao, J.F. Jiang, Y. Luo, C.T. Ma, J.Y. Qian
Due to the morphological similarities of aerial parts, it is difficult to distinguish Gynostemma pentaphyllum from Cayratia japonica, which is usually an adulterant of the former. To develop a reliable method for the identification and authentication of G. pentaphyllum, a combination of random amplification polymorphic DNA (RA.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.8.10
- DOI:
- 10.4238/2015.December.8.10
Pollination of tomatoes by the stingless bee Melipona quadrifasciata and the honey bee Apis mellifera (Hymenoptera, Apidae)
Authors: S.A. Bispo dos Santos, A.C. Roselino,M. Hrncir, L.R. Bego
The pollination effectiveness of the stingless bee Melipona quadrifasciata and the honey bee Apis mellifera was tested in tomato plots. The experiment was conducted in four greenhouses as well as in an external open plot in Ribeirão Preto, SP, Brazil. The tomato plants were exposed to visits by M. quadrifasciata in one .. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2kerr015
- DOI:
- 10.4238/vol8-2kerr015
A novel technique for analyzing the similarity and dissimilarity of DNA sequences
Authors: Y.W. Liu and Y. Peng
li,j denotes the distance between the point (xi, yi) and the point (xj, yi) in graphical representation. By classifying li,j, i, j = 1, 2,…, N according to the number of points between (xi, yi) and (xj, yi), N - 1 types are obtained. The average and variance of every type are assembled by the novel invariant v = (a1, d1, a2, d2,…, aN, dN). C.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.28.2
- DOI:
- 10.4238/2014.January.28.2
Expression and clinical significance of ADAM17 protein in esophageal squamous cell carcinoma
Authors: H.B. Liu, Y. Zhu, Q.C. Yang, Y. Shen, X.J. Zhang and H. Chen
We examined disintegrin and metalloproteinase 17 (ADAM17) protein expression in esophageal squamous cell carcinoma and its clinical and pathological correlated factors. Western blotting and immunohistochemistry were used to detect ADAM17 protein expression in esophageal squamous cell carcinoma and the corresponding normal esophageal mucosa in 50 cases. AD.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.April.30.12
- DOI:
- 10.4238/2015.April.30.12
Screening and identification of peritoneal metastasis-related genes of gastric adenocarcinoma using a cDNA microarray
Authors: F.H. Bai, N.J. Wang, J. Wang, L. Yang, F.M. Zhang, F. Yin, J. Liang, K.C. Wu and D.M. Fan
With the aim of identifying peritoneal metastasis-related genes in gastric cancer, we performed a broad analysis of differential gene expression between the parental cell line GC9811 and its highly metastatic peritoneal counterpart, cell line GC9811-P. Two fluorescent cDNA probes, labeled with Cy3 and Cy5 dyes, were prepared f.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.25.1
- DOI:
- 10.4238/2012.June.25.1
Does beekeeping reduce genetic variability in Melipona scutellaris (Apidae, Meliponini)?
Authors: G.A. Carvalho-Zilse, M.F.F. Costa-Pinto, C.G. Nunes-Silva, W.E. Kerr
Many factors have contributed to reductions in wild populations of stingless bees, such as: deforestation, displacement and destruction of nests by honey gatherers, as well as use of insecticides and other agrochemicals. All of these can potentially affect the populational structure of native species. We analyzed genetic varia.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2keer006
- DOI:
- 10.4238/vol8-2keer006
MKL1-184C>T gene polymorphism is associated with coronary artery diseasein the Chinese Han population
Authors: Y. Xu B. Li, Y.-Z. Bao, X.-Y. Zhang, Z.-Y. Zhu, X.-F. Zhao,Y.-N. Shi, D. Li, S.-H. Chen, Y. Guo and C.-H. Cui
We investigated genetic susceptibility to coronary artery disease (CAD) by studying the association of MKL1 gene polymorphisms with CAD in the Chinese Han population. We performed a case-control study with 476 unrelated CAD patients and 325 non-CAD controls. All SNPs were genotyped with a TaqMan SNP genotyping assay. The distr.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.28.4
- DOI:
- 10.4238/2014.January.28.4
Molecular cloning and expression analysis of TRAF3 in chicken
Authors: H.L. Yang, Z.Q. Feng, S.Q. Zeng, S.M. Li, Q. Zhu and Y.P. Liu
Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.April.30.14
- DOI:
- 10.4238/2015.April.30.14
Comparison of extraction methods of total microbial DNA from freshwater
Authors: P. Li, S.F. Yang, B.B. Lv, K. Zhao, M.F. Lin, S. Zhou, X. Song and X.M. Tang
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugatio.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.16
- DOI:
- 10.4238/2015.January.30.16