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Role of inosine triphosphate pyrophosphatase gene variant on fever incidence during zidovudine antiretroviral therapy
Authors: A.V.C. Coelho, S.P.S. Silva, L. Zandon? , G. Stocco, G. Decorti and S. Crovella
Zidovudine, the antiretroviral drug used to treat HIV infection, commonly causes adverse effects, such as systemic fever and gastrointestinal alterations. In the present study, the potential role of inosine triphosphate pyrophosphatase (ITPA) gene variant on the incidence of adverse events during antiretroviral therapy (ART) o.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019373
- DOI:
- 10.4238/gmr16019373
Genetic variability of watermelon accessions based on microsatellite markers
Authors: R.N.C. de S. Gama C.A.F. Santos R. de C.S. Dias
We analyzed the genetic variability of 40 watermelon accessions collected from 8 regions of Northeastern Brazil using microsatellite markers, in order to suggest strategies of conservation and utilization of genetic variability in this species. These accessions are not commercial cultivars. They were sampled in areas of traditional farmers that usually keep .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.13.3
- DOI:
- 10.4238/2013.March.13.3
Prevalence of thymidylate synthase gene 5'-untranslated region variants in an Argentinean sample
Authors: C. V?¡zquez, M. Orlova, P. Scibona, H. D?az Arce, M.G. Pallotta and W.H. Belloso
Thymidylate synthase (TYMS) is a key enzyme in nucleotide synthesis and therefore, an important target of many chemotherapeutic agents. Expression of TYMS mRNA is thought to be modulated by a 28-bp tandem repeat polymorphism within its 5'-untranslated region, raising the question of this variant’s utility in predicting t.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019367
- DOI:
- 10.4238/gmr16019367
Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay
Authors: L.C. Hermeto, R.J. Oliveira, R. Matuo, P.H.A. Jardim, R. DeRossi, A.C.M.B. Antoniolli, E. Deffune, T.C. Evaristo and ?.E. Santana
The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage patter.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.7
- DOI:
- 10.4238/2015.January.23.7
Chloroplast analysis of Zelkova schneideriana (Ulmaceae): genetic diversity, population structure, and conservation implications
Authors: H.L. Liu1,2, R.Q. Zhang2, M.L. Geng3, J.Y. Zhu1, J.C. An1 and J.L. Ma1
elkova schneideriana is endemic to China and belongs to the Ulmaceae. It is listed as a Near Threatened species in the China Biodiversity Red Data Book. We conducted a phylogeographical study of two chloroplast regions (psbA-trnH and trnG-trnM) in several Chinese Z. schneideriana populations, in order to examine the genetic diversity, population structure.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017739
- DOI:
- 10.4238/gmr.15017739
Cloning and characterization of a farnesyl pyrophosphate synthase from Matricaria recutita L. and its upregulation by methyl jasmonate
Authors: S.S. Su, H.M. Zhang, X.Y. Liu, G.F. Pan, S.P. Ling, X.S. Zhang,X.M. Yang, Y.L. Tai and Y. Yuan
Matricaria recutita (L.), commonly known as chamomile, is one of the most valuable medicinal plants because it synthesizes a large number of pharmacologically active secondary metabolites known as ?±-bisabolol and chamazulene. Although the plant has been well characterized in terms of chemical constituents of essential oil as well as pharmacological proper.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.8
- DOI:
- 10.4238/2015.January.23.8
Effects of sample re-sequencing and trimming on the quality and size of assembled consensus sequences
Authors: Prosdocimi F, Lopes DAO, Peixoto FC, Mourao MM, Pacifico LGG, Ribeiro RA and Ortega JM
The production of nucleic acid sequences by automatic DNA sequencer machines is always associated with some base-calling errors. In order to produce a high-quality DNA sequence from a molecule of interest, researchers normally sequence the same besample many times. Considering base-calling errors as rare events, re-sequencing .. Read More»
- Genet. Mol. Res. 6(4):
Intermediate alleles of Huntingtonâ??s disease HTT gene in different populations worldwide: a systematic review
Authors: T.A. Apolin?¡rio, C.L.A. Paiva and L.A. Agostinho
Huntington’s disease (HD) is an autosomal dominant progressive neurodegenerative disorder caused by a dynamic mutation due to the expansion of CAG repeats in the HTT gene (4p16.3). The considered normal alleles have less than 27 CAG repeats. Intermediate alleles (IAs) show 27 to 35 CAG repeats and expanded alleles have m.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029648
- DOI:
- 10.4238/gmr16029648
Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR
Authors: H.-J. Xiao, J.-H. Jin, W.-G. Chai and Z.-H. Gong
Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L... Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.10
- DOI:
- 10.4238/2015.January.23.10
Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment
Authors: J.Y. Park Y.S. Yoon H.S. Park S.U. Kuh
We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphat.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.4
- DOI:
- 10.4238/2013.March.15.4