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Plant Genetics   Research Article

Discovery of a large set of SNP and SSR genetic markers by high-throughput sequencing of pepper (Capsicum annuum)

Authors: M. Nicola�¯, C. Pisani, J.-P. Bouchet, M. Vuylsteke and A. Palloix

Genetic markers based on single nucleotide polymorphisms (SNPs) are in increasing demand for genome mapping and fingerprinting of breeding populations in crop plants. Recent advances in high-throughput sequencing provide the opportunity for whole-genome resequencing and identification of allelic variants by mapping the reads to a reference genome. However.. Read More»

Genet. Mol. Res. 11(3):
2012.August.13.3
DOI:
10.4238/2012.August.13.3
Plant Genetics   Research Article

Using SCC8, SCF27 and VMC7f2 markers in grapevine breeding for seedlessness via marker assisted selection

Authors: M. Akkurt, A. ��ak�±r, M. Shidfar, B.P. ��elikkol and G. S�¶ylemezo��lu

We used molecular markers associated with seedlessness in grapes, namely SCC8, SCF27 and VMC7f2, to improve the efficiency of seedless grapevine breeding via marker assisted selection (MAS). DNA from 372 F1 hybrid progeny from the cross between seeded “Alphonse Lavallée” and seedless “Sultani” was amplified by PCR using thre.. Read More»

Genet. Mol. Res. 11(3):
2012.August.13.2
DOI:
10.4238/2012.August.13.2
Plant Genetics   Research Article

Molecular characterization of an opossum Didelphis albiventris (Marsupialia: Didelphidae) population in an urban fragment of the Brazilian Atlantic Rainforest and support to species barcode identification

Authors: L.C.C. Sousa, C.M.F. Gontijo, G.A. Lacorte, S.N. Meireles, A.P. Silva and C.G. Fonseca

We made a molecular study of 40 opossums, Didelphis albiventris, from an urban fragment of the Atlantic Rainforest in southeastern Brazil, analyzing a 653-bp sequence of cytochrome c oxidase, subunit I. We found three close connected haplotypes, with low nucleotide diversity and a haplotype diversity of 59.1% and confirmed sympatry between D. albiventris .. Read More»

Genet. Mol. Res. 11(3):
2012.June.15.4
DOI:
10.4238/2012.June.15.4
Animal Genetics   Research Article

Isolation and characterization of microsatellite loci in hybrid giant tiger grouper

Authors: Y.S. Huang, Z.B. Li, Y.F. Ning, J.B. Shangguan, Y. Yuan, X.Q. Mao and B.B. Li

The hybrid giant tiger grouper is a fish that has considerable commercial value and has become increasingly important for aquaculture in South East Asia since 2008. In order to prevent any reduction in genetic diversity in hybrid grouper as a result of aquaculture, we have identified 21 microsatellite markers that can be used .. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.35
DOI:
10.4238/2015.November.18.35
Plant Genetics   Research Article

Relationships between four measures of genetic distance and breeding behavior in spring wheat

Authors: G. Benin, G. Matei, A. Costa de Oliveira, G.O. Silva, T.R. Hagemann, C. Lemes da Silva, E.S. Pagliosa and E. Beche

We estimated the genetic distances among 10 spring wheat genotypes based on pedigree data, morphological traits and AFLP markers, used individually and combined with morphological traits, to find the best predictors of general- and specific-combining abilities among parental genotypes. Ten wheat parents were crossed in a diallel form, disregarding recipro.. Read More»

Genet. Mol. Res. 11(3):
2012.June.15.3
DOI:
10.4238/2012.June.15.3
Medical Genetics   Research Article

VKORC1 rs2359612 and rs9923231 polymorphisms correlate with high risks of cardiovascular and cerebrovascular diseases

Authors: Y. Li, J. Zhu and J.Q. Ding

We aimed to confirm the correlations between rs2359612 and rs9923231 single nucleotide polymorphisms (SNPs) in the vitamin K epoxide reductase complex subunit 1 (VKORC1) gene and the risk of cardiovascular and cerebrovascular diseases (CCVDs) using meta-analysis. Electronic databases were exhaustively searched for rel.. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.38
DOI:
10.4238/2015.November.18.38
Human Genetics   Research Article

Sex chromosome differentiation in Belostoma (Insecta: Heteroptera: Belostomatidae)

Authors: V.B. Bardella, A.L. Dias, L. Giuliano-Caetano, J.R.I. Ribeiro and R. Da Rosa

Belostoma, a genus of the family Belostomatidae, includes species of great ecological importance as biocontrol agents. Few species of these species have been the subject of cytogenetic analyses. Karyotypic evolution in this genus involves agmatoploidy and simploidy; there are also different sex chromosome systems. We examined two Belostoma species (B. dil.. Read More»

Genet. Mol. Res. 11(3):
2012.May.21.2
DOI:
10.4238/2012.May.21.2
Microbial Genetics   Research Article

Fine-scale spatial genetic structure of an endangered marsh herb, Caldesia grandis (Alismataceae)

Authors: X.-L. Yue, J.-M. Chen, Y.-H. Guo and Q.-F. Wang2

The endangered marsh herb, Caldesia grandis, is native to China. We investigated the spatial structure of the genetic variation of three populations of C. grandis using RAPD markers and spatial autocorrelation analysis, based on the method of equal distance interval. A total of 157 individuals were sampled from four patches collected from the region of Hu.. Read More»

Genet. Mol. Res. 11(3):
2012.May.18.10
DOI:
10.4238/2012.May.18.10
Human Genetics   Original Articles

Correlation of Y-chromosome multiple segmental deletions and chromosomal anomalies in non-obstructive azoospermic males from northeastern China

Authors: R.-L. Dai, R.-X. Wang, J.-L. Jin, G.-N. Niu, J.-Y. Lee, S.-B. Li and R.-Z. Liu

We investigated the frequency and types of Y-chromosome microdeletions and chromosomal anomalies in non-obstructive azoospermic and severely oligozoospermic infertile males in northeastern China. The sample consisted of 519 infertile males (456 azoospermic, 63 severely oligozoospermic). PCR assays for Y-chromosome microdeletions and chromosome analysis we.. Read More»

Genet. Mol. Res. 11(3):
2012.May.10.3
DOI:
10.4238/2012.May.10.3
Plant Genetics   Research Article

Alternaric acid stimulates phosphorylation of His-tagged RiCDPK2, a calcium-dependent protein kinase in potato plants

Authors: A. Hassan, T. Okuta, M. Kato, N. Hatsugai, Y. Sano, T. Ishimori, K. Okazaki, M.A. Doullah and M.M. Shah

Calcium-dependent protein kinases (CDPK) are an essential component of plant defense mechanisms against pathogens. We investigated the effect of alternaric acid, a host-specific toxin produced by the plant fungal pathogen Alternaria solani (Pleosporaceae), on a putative plasma membrane and cytosolic kinase RiCDPK2 of potato (Solanum tuberosum) and on hype.. Read More»

Genet. Mol. Res. 11(3):
2012.May.10.1
DOI:
10.4238/2012.May.10.1