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Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR
Authors: H. Li1, D.H. Ni, Y.B. Duan, Y. Chen, J. Li, F.S. Song,L. Li, P.C. Wei and J.B. Yang
Rice false smut (RFS) is an important rice disease that is caused by the pathogen Ustilaginoidea virens. In this study, we developed a real-time polymerase chain reaction (PCR) assay to detect U. virens and to estimate the level of disease. The genomic DNAs of U. virens and rice were extracted together from the rice samples. Real-time PCR assays were perf.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.10.4
- DOI:
- 10.4238/2013.December.10.4
Evaluation of genetic diversity in Chinese kale (Brassica oleracea L. var. alboglabra Bailey) by using rapid amplified polymorphic DNA and sequence-related amplified polymorphism markers
Authors: J. Zhang and L.G. Zhang1
Chinese kale is an original Chinese vegetable of the Cruciferae family. To select suitable parents for hybrid breeding, we thoroughly analyzed the genetic diversity of Chinese kale. Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) molecular markers were used to evaluate the genetic div.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.14.10
- DOI:
- 10.4238/2014.February.14.10
Significant association between lower pulse pressure and increasing levels of a novel type of phospholipid
Authors: C.S. Yao, Q.Z. Wu, H.X. Xu, F. Liu, L.G. Chang, L.C. Song, L.S. Gao and X.L. Meng
The aim of this study was to analyze the association between pulse pressure and a novel type of phospholipid with solubility similar to that of lysophosphatidic acid (LPA), designated as AP, which was reported to be elevated during ischemia. In this cross-sectional study, 416 hypertensive patients and 252 controls aged between.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.21.16
- DOI:
- 10.4238/2014.February.21.16
Shortening of the cell cycle and developmental interruption in a Dictyostelium discoideum cell line due to RNAi-silenced expression of allantoicase
Authors: D.M. Xue and L.S. Hou
The signaling molecules NH3 (unprotonated volatile ammonia), as well as cyclic adenosine monophosphate and differentiationinducing factor, play important roles in the multicellular development of the slime mould Dictyostelium discoideum. One of the downstream metabolic products catalyzed by allantoicase (allC) is ammonia. We o.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.11
- DOI:
- 10.4238/2012.July.19.11
Johanson-Blizzard syndrome: a report of gender-discordant twins with a novel UBR1 mutation
Authors: C.R. Quaio, Y.K. Koda, D.R. Bertola, M. Sukalo, M. Zenker and C.A. Kim
Johanson-Blizzard syndrome (JBS) is a rare autosomal recessive disorder resulting from loss-of-function mutations in the UBR1 gene. JBS can be easily recognized by its unique clinical presentation (including exocrine pancreatic insufficiency, hypoplasia/aplasia of the alae nasi, congenital scalp defects, sensorineural hearing .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.2
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.2
Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury
Authors: C.L. Hou, Q. Huang, Y. Wei, W. Zhang, J.H. Mi, D.J. Ying and Z.H. Zhou
We constructed a plasmid containing a protein transduction domain (PTD) and a human A20 (hA20) gene fragment; the fusion protein was obtained by highly expressing this plasmid in the yeast Pichia pastoris GS115. The plasmid was obtained by adding 9xArg and EcoRÐ? recognition sites to the end of the primer, and 6xHis-Tag and .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.9
- DOI:
- 10.4238/2012.July.19.9
Molecular cloning and cDNA characterization of Camelus dromedarius putative cytochrome P450s 1A, 2C, and 3A
Authors: H.M. Saeed, M.S. Alanazi, M.A. Shalaby, O. Alshahrani, F.S. Ataya, A.A. Pathan and Z.A Abduljaleel
The domesticated one-humped Arabian camel, Camelus dromedarius, is one of the most important animals in the Arabian Peninsula. Most of its life, this animal is exposed to both intrinsic and extrinsic genotoxic factors that are known to cause gross metabolic alterations in many organisms. This study determined the full length c.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.March.17.1
- DOI:
- 10.4238/2014.March.17.1
Analysis of geographic and pairwise distances among sheep populations
Authors: J.B. Liu, Y.J. Yue, X. Lang, F. Wang, X. Zha, J. Guo, R.L. Feng, T.T. Guo, B.H. Yang and X.P. Sun
This study investigated geographic and pairwise distances among seven Chinese local and four introduced sheep populations via analysis of 26 microsatellite DNA markers. Genetic polymorphism was rich, and the following was discovered: 348 alleles in total were detected, the average allele number was 13.38, the polymorphism info.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.4
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.4
Transcriptome profiling of the crofton weed gall fly Procecidochares utilis
Authors: X. Gao, J.Y. Zhu, S. Ma, Z. Zhang, C. Xiao, Q. Li, Z.Y. Li and G.X. Wu
Procecidochares utilis is a tephritid gall fly, which is known to be an effective biological agent that can be used to control the notoriously widespread crofton weed Eupatorium adenophorum. Despite its importance, genetic resources for P. utilis remain scarce. In this study, 1.2 Gb sequences were generated using Illumina pair.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.March.19.1
- DOI:
- 10.4238/2014.March.19.1
Water buffalo genome characterization by the Illumina BovineHD BeadChip
Authors: R.R.A. Borquis, F. Baldi, G.M.F. de Camargo, D.F. Cardoso, D.J.A. Santos, N.H. Lugo, M. Sargolzaei, F.S. Schenkel, L.G. Albuquerque and H. Tonhati
To define the best strategies for genomic association studies and genomic selection, it is necessary to determine the extent of linkage disequilibrium (LD) and the genetic structure of the study population. The current study evaluated the transference of genomic information contained in the Illumina BovineHD BeadChip from catt.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.6
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.6