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Human Genetics   Research Article

Genetic analysis of 30 InDel markers for forensic use in five different Chinese populations

Authors: C.T. Li, S.H. Zhang and S.M. Zhao

Allele frequencies of 30 insertion/deletion polymorphism (InDel) markers previously selected and validated for forensic purposes were assessed in 419 unrelated individuals originating from five different populations of P.R. China, including Chinese Han, Chinese Hui, Uighur, Mongolians, and Tibetans. Hardy-Weinberg equilibrium .. Read More»

Genet. Mol. Res. 10(2):
vol10-2gmr1082
DOI:
10.4238/vol10-2gmr1082
Plant Genetics   Research Article

Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Authors: R.M. Al-Atiyat and R.S. Aljumaah

This study aimed to estimate evolutionary distances and to reconstruct phylogeny trees between different Awassi sheep populations. Thirty-two sheep individuals from three different geographical areas of Jordan and the Kingdom of Saudi Arabia (KSA) were randomly sampled. DNA was extracted from the tissue samples and sequenced u.. Read More»

Genet. Mol. Res. 13(3):
2014.August.26.6
DOI:
10.4238/2014.August.26.6
Human Genetics   Research Article

H558R polymorphism in SCN5A is associated with Keshan disease and QRS prolongation in Keshan disease patients

Authors: S. Jiang, F.L. Li, Q. Dong, H.W. Liu, C.F. Fang, C. Shu, H. Cheng, J. Cui, H.X. Ma, D.Q. Chen, H. Li

Keshan disease (KSD), a potentially fatal cardiomyopathy, has very high incidence in some selenium-poor regions of China. KSD may be accompanied with a variety of arrhythmia, which is associated with mutations in the gene coding for cardiac voltage-gated sodium channel (SCN5A). The molecular mechanism of KSD is still .. Read More»

Genet. Mol. Res. 13(3):
2014.August.28.1
DOI:
10.4238/2014.August.28.1
Plant Genetics   Research Article

Cloning and expression analysis of the Lonicera japonica Thunb. chlorogenic acid synthetase gene (LjCCoAOMT1) in rice

Authors: X.-H Jiang , C.-W She, Y.-H Zhu and X.-M Liu

Complete coding DNA sequences of a closely related chlorogenic acid synthetase gene (LjCCoAOMT1) were isolated from Lonicera japonica Thunb. by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). LjCCoAOMT1 was subsequently overexpressed in Escherichia coli and a 25-kD protein .. Read More»

Genet. Mol. Res. 13(1):
2014.March.26.5
DOI:
10.4238/2014.March.26.5
Plant Genetics   Research Article

Bioinformatic analysis of the nucleotide binding site-encoding disease-resistance genes in foxtail millet (Setaria italica (L.) Beauv.)

Authors: Y.B. Zhu, X.Q. Xie, Z.Y. Li, H. Bai, L. Dong, Z.P. Dong and J.G. Dong

The nucleotide-binding site (NBS) disease-resistance genes are the largest category of plant disease-resistance gene analogs. The complete set of disease-resistant candidate genes, which encode the NBS sequence, was filtered in the genomes of two varieties of foxtail millet (Yugu1 and ‘Zhang gu’). This study invest.. Read More»

Genet. Mol. Res. 13(3):
2014.August.28.5
DOI:
10.4238/2014.August.28.5
Plant Genetics   Research Article

Molecular cloning, sequence analysis, prokaryotic expression, and function prediction of foot-specific peroxidase in Hydra magnipapillata Chinese strain

Authors: H.C. Pan, H.Q. Yang, F.X. Zhao and X.C. Qian

The cDNA sequence of foot-specific peroxidase PPOD1 from the Chinese strain of Hydra magnipapillata was cloned by reverse transcription-polymerase chain reaction. The cDNA sequence contained a coding region with an 873-bp open reading frame, a 31-bp 5'-untranslated region, and a 36-bp 3'-untranslated region. The struc.. Read More»

Genet. Mol. Res. 13(3):
2014.August.28.6
DOI:
10.4238/2014.August.28.6
Animal Genetics   Research Article

Isolation and characterization of 21 novel polymorphic microsatellite loci in the Chinese soft-shelled turtle Pelodiscus sinensis

Authors: X.J. Bu, L. Liu, L. Wang and L.W. Nie

Twenty-one novel polymorphic microsatellite loci were isolated from the Chinese soft-shelled turtle, Pelodiscus sinensis (Trionychidae). This is a commonly consumed Asian species, especially in China, where it is reared in large numbers on farms. We screened 34 unrelated individuals from Wuhu of Anhui province in China and det.. Read More»

Genet. Mol. Res. 10(2):
vol10-2gmr1119
DOI:
10.4238/vol10-2gmr1119
Plant Genetics   Research Article

Anatomic changes due to interspecific grafting in cassava (Manihot esculenta)

Authors: N. Bomfim, D.G. Ribeiro and N.M.A. Nassar

Cassava rootstocks of varieties UnB 201 and UnB 122 grafted with scions of Manihot fortalezensis were prepared for anatomic study. The roots were cut, stained with safranin and alcian blue, and examined microscopically, comparing them with sections taken from ungrafted roots. There was a significant decrease in number of peric.. Read More»

Genet. Mol. Res. 10(2):
vol10-2gmr1138
DOI:
10.4238/vol10-2gmr1138
Plant Genetics   Research Article

Devolopmental and growth temperature regulation of omega-3 fatty acid desaturase genes in safflower (Carthamus tinctorius L.)

Authors: L.-L. Guan, W. Wu, B. Hu, D. Li, J.-W. Chen, K. Hou and L. Wang

Three ω-3 fatty acid desaturase genes (CtFAD3, CtFAD7, and CtFAD8) were isolated from safflower (Carthamus tinctorius L.). Transcript analysis showed that the highest transcript levels were detected for CtFAD3 and the low transcript levels were detected for CtFAD7 and.. Read More»

Genet. Mol. Res. 13(3):
2014.August.28.7
DOI:
10.4238/2014.August.28.7
Medical Genetics   Research Article

Locally linear embedding and neighborhood rough set-based gene selection for gene expression data classification

Authors: L. Sun, J.-C. Xu, W. Wang and Y. Yin

Cancer subtype recognition and feature selection are important problems in the diagnosis and treatment of tumors. Here, we propose a novel gene selection approach applied to gene expression data classification. First, two classical feature reduction methods including locally linear embedding (LLE) and rough set (RS) are summar.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038990
DOI:
10.4238/gmr.15038990