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Nanofibers promote HepG2 aggregate formation and cellular function
Authors: W.T. Su, Y.J. Liu and T.Y. Huang
Formation of hepatocyte spheroids is a necessary strategy for increasing liver-specific function in vitro. In this study, HepG2 cells showed good viability when grown on a polylactic acid-chitosan (PLA-CS) nanofiber and aggregated to form multicellular spheroids on the PLA-CS nanofibers with a diameter of approximately 100-200 mm in 5 days of culture, whe.. Read More»
- Genet. Mol. Res. 15(3):
Highly efficient one-step PCR-based mutagenesis technique for large plasmids using high-fidelity DNA polymerase
Authors: H. Liu, R. Ye and Y.Y. Wang
Gene mutation plays an important role in molecular biological studies. A highly efficient one-step polymerase chain reaction-based mutagenesis technique for site-directed mutagenesis was developed in this study. One complementary pair of primers was designed that contained the desired mutations in the middle of the primers. The amplification products of m.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.10
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.10
Potassium contributes to zinc stress tolerance in peach (Prunus persica) seedlings by enhancing photosynthesis and the antioxidant defense system
Authors: Z.Z. Song, C.L. Duan, S.L. Guo, Y. Yang, Y.F. Feng, R.J. Ma and M.L. Yu
Zinc (Zn) is considered to be a major industrial pollutant because excessive amounts can impair plant growth. In this paper, we found that peach ‘Yoshihime’ seedlings are promising Zn tolerant plants. However, heavy Zn toxicity (2 mM) damaged plant performance by disrupting biochemical processes, including photosynthesis, proline production, a.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.22
- DOI:
- 10.4238/2015.July.27.22
Relationship between EPHX2 gene polymorphisms and essential hypertension in Uygur, Kazakh, and Han
Authors: X.L. Zhu, L. Wang, Z. Wang, S.Z. Chen, W.Q. Zhang and M.M. Ma
We investigated the association between rs751141 polymorphisms in the EPHX2 gene and essential hypertension in Uygur, Kazakh, and Han subjects in Xinjiang, China. A total of 302 essential hypertensive patients in Uygur, 267 in Kazakh, and 368 in Han, as well as 323 normotensive controls in Uygur, 284 in Kazakh, and 348 in Han were enrolled in this study. .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.11
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.11
Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima
Authors: W. Yan, L. Liu, C.X. Li, S.C. Huang, Z.L. Ma, W.Q. Qin and Z.Q. Peng
The coconut leaf beetle, Brontispa longissima, is a destructive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular basis of biology and ecology, we sequenced and analyzed its whole transcriptome by using high-through.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.2
- DOI:
- 10.4238/2015.July.28.2
Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines
Authors: J. Ogorevc and P. Dov?
Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.12
Association between methionine synthase reductase A66G polymorphism and primary infertility in Chinese males
Authors: X.Y. Li, J.Z. Ye, X.P. Ding, X.H. Zhang, T.J. Ma, R. Zhong and H.Y. Ren
We examined the association between the methionine synthase reductase (MTRR A66G), methylenetetrahydrofolate reductase (MTHFR C677T and A1298C), and methionine synthase (MS A2756G) genotypes and non-obstructive male infertility in a Chinese population. This case-control study included 162 infertile Chinese patients with azoospermia (N = 100) or oligoasthe.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.13
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.13
Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene
Authors: H.Y. Zhang, H.M. Liu and X.Z. Liu
The kiwifruit (Actinidia chinensis Planch.) is an economically and nutritionally important fruit crop that has a remarkably high vitamin C content and is popular throughout the world. However, kiwifruit plants are vulnerable to attack from pests, and effective pest control is urgently required. Transgenic kiwifruit plants containing the synthetic chimeric.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.16
- DOI:
- 10.4238/2015.July.28.16
Polymorphisms in the methylene tetrahydrofolate reductase and methionine synthase reductase genes and their correlation with unexplained recurrent spontaneous abortion susceptibility
Authors: L. Zhu
We aimed to explore the correlation between unexplained recurrent spontaneous abortion and polymorphisms in the methylene tetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) genes. A case control study was conducted in 118 patients with unexplained recurrent spontaneous abortion (abortion group) and 174 healthy women (control group.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.19
- DOI:
- 10.4238/2015.July.28.19
Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA
Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.20
- DOI:
- 10.4238/2015.July.28.20