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Repair of articular cartilage defects by tissue-engineered cartilage constructed with adipose-derived stem cells and acellular cartilaginous matrix in rabbits
Authors: Z.J. Wang, R.Z. An, J.Y. Zhao, Q. Zhang, J. Yang, J.B. Wang, G.Y. Wen, X.H. Yuan, X.W. Qi, S.J. Li and X.C. Ye
After injury, inflammation, or degeneration, articular cartilage has limited self-repair ability. We aimed to explore the feasibility of repair of articular cartilage defects with tissue-engineered cartilage constructed by acellular cartilage matrices (ACMs) seeded with adipose-derived stem cells (ADSCs). The ADSCs were isolat.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.18.2
- DOI:
- http://dx.doi.org/10.4238/2014.June.18.2
Snail-induced epithelial-mesenchymal transition in gastric carcinoma cells and generation of cancer stem cell characteristics
Authors: Y.J. Yang, Z.B. Li, G.R. Zhang, L.J. Wu, J.Y. Yu, L.J. Hu, Y.L. Zhou, H.D. Wang and D. Liang
Biological changes in Snail-overexpressed SGC7901 cells were studied by establishing a pEGFP-C1-Snail carrier. The significance of Snail in epithelial-mesenchymal transition (EMT) as well as the invasion and metastatic capacity of gastric cancer cells was also discussed; moreover, we attempted to verify the probable cancer ste.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038510
- DOI:
- 10.4238/gmr.15038510
Molecular cloning and functional characterization of a glutathione S-transferase involved in both anthocyanin and proanthocyanidin accumulation in Camelina sativa (Brassicaceae)
Authors: Y. Wang, Y. Tang, M. Zhang, F. Cai, J. Qin, Q. Wang, C. Liu, G. Wang, L. Xu, L. Yang, J. Li, Z. Wang and X. Li
Recently, we found that the Arabidopsis TT19 protein, a glutathione S-transferase, has two functional domains that influence both anthocyanin and proanthocyanidin accumulation. To further understand the function of this protein in the other species, we cloned a cDNA encoding a glutathione S-transferase (namely CMGSTF12) from Camelina sativa, an oil crop t.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.25.4
- DOI:
- 10.4238/2012.September.25.4
Development of SNP-based dCAPS markers for identifying male sterile gene tms5 in two-line hybrid rice
Authors: F.S. Song, J.L. Ni1, Y.L. Qian, L. Li, D.H. Ni and J.B. Yang
Molecular markers can increase both the efficiency and speed of breeding programs. Functional markers that detect the functional mutations causing phenotypic changes offer a precise method for genetic identification. In this study, we used newly derived cleaved amplified polymorphic sequence markers to detect the functional mu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038512
- DOI:
- 10.4238/gmr.15038512
Safety of recombinant human granulocyte-macrophage colony-stimulating factor in healing pediatric severe burns
Authors: Y.F. Chi, J.K. Chai, H.M. Luo, Q.X. Zhang and R. Feng
We explored the safety of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) for healing burns in children. Subjects were randomly assigned to two groups: the experimental group received external rhGM-CSF gel, and the control group received rhGM-CSF gel matrix components, applied to the burn surface. Neither group was given any .. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.31.3
- DOI:
- http://dx.doi.org/10.4238/2015.March.31.3
Programmed cell death in salivary glands of Drosophila arizonae and Drosophila mulleri
Authors: P. Ianella, M.T.V. Azeredo-Oliveira and M.M. Itoyama
Programmed cell death (PCD) in insect metamorphosis assumes a great diversity of morphology and controlling processes that are still not well understood. With the objective of obtaining information about the PCD process, salivary glands of Drosophila arizonae and D. mulleri were studied during larval-pupal development. From the results, it can be conclude.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr412
- DOI:
- 10.4238/vol7-2gmr412
Induction of mitotic crossing-over in diploid strains of Aspergillus nidulans using low-dose X-rays
Authors: L.B. Stoll, F.C.A. Cremonesi, L.T.A. Pires, T.D. Zucchi andT.M.A.D. Zucchi4,
As a contribution towards detecting the genetic effects of low doses of genotoxic physical agents, this paper deals with the consequences of low-dose X-rays in the Aspergillus nidulans genome. The irradiation doses studied were those commonly used in dental clinics (1-5 cGy). Even very low doses promoted increased mitotic crossing-over frequencies in dipl.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr414
- DOI:
- 10.4238/vol7-2gmr414
Cloning, sequencing and antigenic characterization of rVirB9 of Anaplasma marginale isolated from Paran�¡ State, Brazil
Authors: M.C. Vidotto, E.J. Ven�¢ncio and O. Vidotto
Anaplasma marginale, a tick-borne bacterium, causes bovine anaplasmosis responsible for significant economic losses in tropical and subtropical regions worldwide. Various major outer membranes have been described, and VirB9, a type IV secretion system protein, has been recently indicated as a candidate in vaccine development against anaplasmosis. The virB.. Read More»
- Genet. Mol. Res. 7(2):
A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency
Authors: L.H. Zhao, Y.H. Zhao, H. Liang, T. Yun, X.J. Han, M.L. Zhang, X. Zhou, D.X. Hou, R.F. Li and X.L. Li
With the development of gene targeting approaches, genomic mutation technologies in livestock animals such as gene trapping, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats and their associated systems have been improved. Although ZFNs have been used for gene targ.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.31.5
- DOI:
- http://dx.doi.org/10.4238/2015.March.31.5
Genotoxicity evaluation in chronic renal patients undergoing hemodialysis and peritoneal dialysis, using the micronucleus test
Authors: J.M. Roth, R.G. Restani, T.T.S. Gon�§alves, S.L.S. Sphor,A.B. Ness, M.G. Martino-Roth and G.L. Garcias
Patients with chronic renal disease have an increased incidence of cancer. It is well known that long periods of hemodialysis treatment are linked to DNA damage due to oxidative stress. This genotoxic effect may cause the loss of chromosome fragments, or even entire chromosomes, which form micronuclei after cell division, and .. Read More»
- Genet. Mol. Res. 7(2):