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cDNA, genomic sequence cloning and overexpression of ribosomal protein gene L9 (rpL9) of the giant panda (Ailuropoda melanoleuca)
Authors: W.R. Hou, Y.L. Hou, G.F. Wu, Y. Song, X.L. Su, B. Sun and J. Li
The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mam.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1159
- DOI:
- 10.4238/vol10-3gmr1159
In silico prediction of yeast deletion phenotypes
Authors: Soma Saha and Steffen Heber
Analysis of gene deletions is a fundamental approach for investigating gene function. We evaluated an algorithm that uses classification techniques to predict the phenotypic effects of gene deletions in yeast. We used a modified simulated annealing algorithm for feature selection and weighting. The selected features with high .. Read More»
- Genet. Mol. Res. 5(1):
DBCollHIV: a database system for collaborative HIV analysis in Brazil
Authors: Luciano V. Ara�ºjo, Marcelo A. Soares, Suelene M. Oliveira, Pedro Chequer, Amilcar Tanuri, Ester C. Sabino and Jo�£o E. Ferreira
We developed a database system for collaborative HIV analysis (DBCollHIV) in Brazil. The main purpose of our DBCollHIV project was to develop an HIV-integrated database system with analytical bioinformatics tools that would support the needs of Brazilian research groups for data storage and sequence analysis. Whenever authoriz.. Read More»
- Genet. Mol. Res. 5(1):
Analysis of slipped sequences in EST projects
Authors: Christian Baudet and Zanoni Dias
Slippage is an important sequencing problem that can occur in EST projects. However, very few studies have addressed this. We propose three new methods to detect slippage artifacts: arithmetic mean method, geometric mean method, and echo coverage method. Each method is simple and has two different strategies for processing seq.. Read More»
- Genet. Mol. Res. 5(1):
Karyotypic characterization of Iheringichthys labrosus (Pisces, Pimelodidae): C-, G- and restriction endonuclease banding
Authors: Rafael Augusto de Carvalho and Ana L�ºcia Dias
Various chromosomal banding techniques were utilized on the catfish, Iheringichthys labrosus, taken from the Capivara Reservoir. C-banding regions were evidenced in telomeric regions of most of the chromosomes. The B microchromosome appeared totally heterochromatic. The restriction endonuclease AluI produced a banding pattern .. Read More»
- Genet. Mol. Res. 4(4):
Simplified preparation of a DNA ladder using PCR
Authors: T.-Y. Wang, L. Wang and F. Wang
Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1177
- DOI:
- 10.4238/vol10-3gmr1177
Genome wide cloning of maize meiotic recombinase Dmc1 and its functional structure through molecular phylogeny
Authors: F. Etedali, B. Baghban Kohnehrouz, M. Valizadeh, A. Gholizadeh and M.A. Malboobi
The development of meiotic division and associated genetic recombination paved the way for evolutionary changes. However, the secondary and tertiary structure and functional domains of many of the proteins involved in genetic recombination have not been studied in detail. We used the human Dmc1 gene product along with secondar.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1338
- DOI:
- 10.4238/vol10-3gmr1338
Hydrolases in the hypopharyngeal glands of workers of Scaptotrigona postica and Apis mellifera (Hymenoptera, Apinae)
Authors: Rosil�©ia A.C. Costa and Carminda da Cruz-Landim
Hydrolytic enzymes from hypopharyngeal gland extracts of newly emerged, nurse and foraging workers of two eusocial bees, Scaptotrigona postica, a native Brazilian stingless bee, and the Africanized honey bee (Apis mellifera) in Brazil, were compared. The hypopharyngeal gland is rich in enzymes in both species. Fifteen differen.. Read More»
- Genet. Mol. Res. 4(4):
Problems with and a system to eliminate single-primer PCR product contamination in simple sequence repeat molecular marker-assisted selection in soybean
Authors: J. Ma, S.C. Guan, D. Yao, Y.F. Wei and P.W. Wang
Polymerase chain reaction (PCR) provides a foundation for simple sequence repeat molecular marker-assisted selection (SSR MAS) in soybean. This PCR system and its various conditions have been optimized by many researchers. However, current research on the optimization of the PCR system focuses on double-primer PCR products. We.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1366
- DOI:
- 10.4238/vol10-3gmr1366
A modified method for high-quality DNA extraction for molecular analysis in cereal plants
Authors: Z.H. Pervaiz, N.A. Turi, I. Khaliq, M.A. Rabbani and S.A. Malik
Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1346
- DOI:
- 10.4238/vol10-3gmr1346