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Role of IL-8 gene polymorphisms in glioma development in a Chinese population
Authors: J.W. Fu, K.W. Wang and S.T. Qi
This case-control study aimed to investigate the role of -251 T>A (rs4073) and -781 C>T (rs2227306) polymorphisms in the interleukin-8 (IL-8) gene in the development of glioma in a Chinese population. One hundred and twenty-seven glioma patients and 284 healthy control subjects were recruited to this study between February 2013 and December 2014. Th.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038127
- DOI:
- 10.4238/gmr.15038127
Development of RAPD-SCAR markers for different Ganoderma species authentication by improved RAPD amplification and molecular cloning
Authors: J.J. Fu, M.A. Khan, Z.Q. Mei, M. Tania, L.Q. Yang and J.L. Cheng
The sequence-characterized amplified region (SCAR) is a valuable molecular technique for the genetic identification of any species. This method is mainly derived from the molecular cloning of the amplified DNA fragments achieved from the random amplified polymorphic DNA (RAPD). In this study, we collected DNA from 10 species of Ganoderma mushroom and ampl.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.19
- DOI:
- 10.4238/2015.May.25.19
Histone H3K9 acetylation influences growth characteristics of goat adipose-derived stem cells in vitro
Authors: X. Wang, F.X. Zhang, Z.M. Wang, Q. Wang, H.F. Wang, Y. Ren,D.P. Tai, H. Liang and D.J. Liu
Adipose-derived stem cells (ADSCs) show nearly unlimited potential in medical and animal science. Currently, understanding of the biological mechanisms regulating ADSC growth in vitro remains very limited. Histone acetylation, an epigenetic modification, plays a key role in maintaining stem cell properties. To further study it.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048954
- DOI:
- 10.4238/gmr15048954
Combination of IL-6, IL-10, and MCP-1 with traditional serum tumor markers in lung cancer diagnosis and prognosis
Authors: Y.W. Pan, Z.G. Zhou, M. Wang, J.Q. Dong, K.P. Du, S. Li, Y.L. Liu, P.J. Lv and J.B. Gao
Early detection and treatment is critically important for lung cancer patients. Inflammatory mediators such as IL-6, IL-10, and MCP-1 participate in lung cancer regulation. CEA, CA125, and ProGRP are commonly used serum tumor markers for lung cancer. In this study, we assessed the sensitivity and specificity of CEA, CA125, and.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048949
- DOI:
- 10.4238/gmr15048949
Wnt7b gene expression and functional analysis in the mussel Mytilus coruscus
Authors: Y.F. Xu, X. Liang, Y.R. Chen, Y.F. Li and J.L. Yang
To understand the potential functions of Wnt7b in different developmental stages and adult tissues of the mussel Mytilus coruscus, the Wnt7b gene was cloned using the rapid amplification of cDNA ends technique. The full-length Wnt7b gene was 1910 bp long, with a 1059-bp open reading frame encoding 352 amino acids. The amino ac.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048866
- DOI:
- 10.4238/gmr15048866
Development and characterization of microsatellite markers for Brazilian four-eyed frogs (genus Pleurodema) endemic to the Caatinga biome
Authors: M.T.C. Thom�©, J. Alexandrino, S. Lopes, C.F.B. Haddad and F. Sequeira
We used pyrosequencing to develop microsatellite markers for the Brazilian four-eyed frog Pleurodema diplolister and tested the microsatellite markers for cross-amplification in its sister Pleurodema alium, which are both endemic species of the Caatinga biome in northeastern Brazil. We used multiplex sets to amplify and genoty.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.12.12
- DOI:
- http://dx.doi.org/10.4238/2014.March.12.12
Genetic analysis of molecular markers for propamocarb residue in Cucumis sativus using quantitative trait locus mapping
Authors: M. Xin, L. Wang, B.H. Ma, Z.W. Qin and X.Y. Zhou
The use of pesticides to protect plants against harmful organisms, such as pathogenic microorganisms, is one of the most effective ways to improve agricultural production. However, the continuous use of pesticides might present a risk to human health, animals, and the environment. In this study, two cucumber (Cucumis sativus) .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048776
- DOI:
- 10.4238/gmr15048776
Limitations of cytochrome oxidase I for the barcoding of Neritidae (Mollusca: Gastropoda) as revealed by Bayesian analysis
Authors: S.Y. Chee
The mitochondrial DNA (mtDNA) cytochrome oxidase I (COI) gene has been universally and successfully utilized as a barcoding gene, mainly because it can be amplified easily, applied across a wide range of taxa, and results can be obtained cheaply and quickly. However, in rare cases, the gene can fail to distinguish between species, particularly when expose.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.20
- DOI:
- 10.4238/2015.May.25.20
Isolation and characterization of polymorphic microsatellite markers in the endangered species Bretschneidera sinensis Hemsl.
Authors: M. Li, H.F. Chen, Z.F. Wang and S. Zhang
Bretschneidera sinensis is an endangered species that is mainly distributed in South China. As a tertiary relict and the single species in the Bretschneideraceae family, it has a high conservation value. To investigate the influence of human disturbance on its mating system, 63 new microsatellites were developed using restriction-site-associated DNA seque.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038234
- DOI:
- 10.4238/gmr.15038234
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows
Authors: I. Fonseca, G.R. Antunes, D.S. Paiva, C.C. Lange, S.E.F. Guimar�£es and M.F. Martins
Among the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resis.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1096
- DOI:
- 10.4238/vol10-3gmr1096