Se

Development of a cyclic voltammetry method for the detection of Clostridium novyi in black disease

Authors: L.L. Liu, D.N. Jiang, G.M. Xiang, C. Liu, J.C. Yu and X.Y. Pu

Black disease is an acute disease of sheep and cattle. The pathogen is the obligate anaerobe, Clostridium novyi. Due to difficulties of anaerobic culturing in the country or disaster sites, a simple, rapid, and sensitive method is required. In this study, an electrochemical method, the cyclic voltammetry method, basing on loop.. Read More»

Genet. Mol. Res. 13(1):

http://dx.doi.org/2014.January.14.9

DOI:

http://dx.doi.org/10.4238/2014.January.14.9

RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass

Authors: R.S.N de Lima, R.F. Daher, L.S.A. Gon�§alves, D.A. Rossi, A.T. do Amaral J�ºnior, M.G. Pereira and F.J.S. L�©do

Considering the expected genetic variability of elephant grass (Pennisetum purpureum), due to its cultivation in different continents, we characterized and estimated the genetic divergences between 46 accessions of elephant grass with different edaphoclimatic adaptations, using RAPD and ISSR markers. We evaluated, comparativel.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1107

DOI:

10.4238/vol10-3gmr1107

Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)

Authors: R.C.C. Vasconcellos, T.F.C. Lima, C.N. Fernandes-Brum, A. Chalfun-Junior and J.B. Santos

The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses. This study aimed to i) investigate variation in the expression of different common bean (Phaseolus vulgaris L.) genotypes and its re.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038269

DOI:

10.4238/gmr.15038269

DNA barcoding and phylogenetic relationships of Ardeidae (Aves: Ciconiiformes)

Authors: Z.H. Huang, M.F. Li and J.W. Qin

The avian family Ardeidae comprises long-legged freshwater and coastal birds. There has been considerable disagreement concerning the intrafamilial relationships of Ardeidae. Mitochondrial cytochrome c oxidase subunit I (COI) was used as a marker for the identification and phylogenetic analysis of avian species. In the present study, we analyzed the COI b.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038270

DOI:

10.4238/gmr.15038270

Mapping, expression and regulation of the TRa gene in porcine adipose tissue

Authors: Z.-W. Cai, Y.-F. Sheng, L.-F. Zhang, Y. Wang, X.-L. Jiang, Z.-Z. Lv and N.-Y. Xu

Thyroid hormone receptors (TR) are members of the nuclear receptor superfamily. There are at least two TR isoforms, TRα and TRβ. The TRα isoform plays a critical role in mediating the action of thyroid hormone in adipose tissue. We mapped the porcine TRα gene to chromosome 12 p11-p13, by using the ImpRH .. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1113

DOI:

10.4238/vol10-3gmr1113

Characterization and identification of ISSR markers associated with oil content in sea buckthorn berries

Authors: J. Ding, C.J. Ruan, Y. Guan, J.Y. Shan, H. Li and Y.H. Bao

Bioactive oils extracted from sea buckthorn (Hippophae rhamnoides) berries contain highly nutritional and medicinal compounds; however, the oil contents of sea buckthorn berries are very low. Thirteen inter-simple sequence repeat (ISSR) primers were used to identify markers associated with oil content of dry pulp in 51 cultivars and lines, which clustered.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038278

DOI:

10.4238/gmr.15038278

Association of MHC class-III gene polymorphisms with ER-positive breast cancer in Chinese Han population

Authors: Q. Pan Y. Ning L.Z. Chen S. Zhang Z.Z. Liu X.X. Yang W. Wei H. Wei Q.G. Li H.N. Yue J.X. Wang

Polymorphisms of the major histocompatibility complex (MHC) have been linked to many diseases, especially autoimmune disorders. Previous studies have shown that genetic variants in MHC class III are associated with breast cancer. To determine if there is an association between MHC class III and breast cancer risk in the Chinese Han population, we carried .. Read More»

Genet. Mol. Res. 11(4):

2012.September.17.1

DOI:

10.4238/2012.September.17.1

Characterization and heterologous expression of isocitrate lyase of Paracoccidioides brasiliensis

Authors: A.H.S. Cruz

Genet. Mol. Res. 11(4):

2012.December.17.5

DOI:

10.4238/2012.December.17.5

Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa)

Authors: S. Ashkani, M.Y. Rafii, M. Sariah, A. Siti Nor Akmar, I. Rusli, H. Abdul Rahim and M.A. Latif

Among 120 simple sequence repeat (SSR) markers, 23 polymorphic markers were used to identify the segregation ratio in 320 individuals of an F2 rice population derived from Pongsu Seribu 2, a resistant variety, and Mahsuri, a susceptible rice cultivar. For phenotypic study, the most virulent blast (Magnaporthe oryzae) pathotype.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1331

DOI:

10.4238/vol10-3gmr1331

Clinical and molecular genetic study of 12 Italian families with autosomal recessive Stargardt disease

Authors: M. Oldani S. Marchi A. Giani S. Cecchin E. Rigoni A. Persi D. Podavini A. Guerrini A. Nervegna G. Staurenghi M. Bertelli

Stargardt disease was diagnosed in 12 patients from 12 families using complete ophthalmologic examination, fundus photography, fundus autofluorescence, and spectral-domain optical coherence tomography. DNA was extracted for polymerase chain reaction (PCR) and direct DNA sequencing (ABCA4 gene). Genetic counseling and eye examination were offered to 16 add.. Read More»

Genet. Mol. Res. 11(4):

2012.October.9.3

DOI:

10.4238/2012.October.9.3