Se
Isolation, partial characterization, and cloning of an extracellular chitinase from the entomopathogenic fungus Verticillium lecanii
Authors: G. Yu, L.Q. Xie, J.T. Li, X.H. Sun, H. Zhang, Q. Du, Q.Y. Li,S.H. Zhang and H.Y. Pan
The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent of fungal phytopathogens, as well as insect pests. A 42-kDa chitinase belonging to family 18 of the glycosyl hydrolases was isolated and partially characterized. Chitinase was purified using successive column chromatography on phenyl-sepharose, DEAE-sepharose, and CM-sepharo.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.13
- DOI:
- 10.4238/2015.March.27.13
Molecular marker-assisted selection of the ae alleles in maize
Authors: F. Chen, S.W. Zhu, Y. Xiang, H.Y. Jiang and B.J. Cheng
The ae (amylose extender) recessive mutant alleles in maize are an important genetic resource for the development of high-amylose cultivars. On the basis of ae allele sequences (from the National Center for Biotechnology Information), the ae mutant alleles were cloned from high-amylose maize and the allelic Ae gene from common.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr799
- DOI:
- 10.4238/vol9-2gmr799
Molecular cloning, characterization, and expression of vacuolar-type-H+-ATPase B1 (VHAB1) gene in the gill of Anguilla marmorata
Authors: L. Li, Y.H. Jia, P. Li, S.W. Yin, G.S. Zhang, X.L. Wang, Y.Y. Wang and X.J. Wang
We explored the molecular mechanism of the regulation of vacuolar-type-H+-ATPase B1 (VHAB1) in elvers in the response to salinity. The full-length cDNA of VHAB1 in Anguilla marmorata (designated as AmVHAB1), which was 1741 base pairs (bp) in length, was found to encompass a 1512-bp open reading frame encoding a polypeptide with 503 amino acids (55.9 kDa),.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.9
- DOI:
- 10.4238/2015.July.17.9
Screening of differentially-expressed genes in the muscles of rabbit breeds with expression profile chip
Authors: W.Z. Wang, T. Li, L.J. Shi, X.R. Yan, Y.L. Pan and X.S. Wu
The molecular mechanism underlying muscle development in rabbits is not well-understood. In the current study, differentially-expressed genes were scanned using an expression profile chip in New Zealand white rabbits (introduced breed) and Fujian yellow rabbits (local breed), and some of the genes were tested using reverse transcription-polymerase chain r.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.12
- DOI:
- 10.4238/2015.July.17.12
Meta-analysis of angiotensin-converting enzyme insertion/deletion polymorphism and myocardial infarction in Han Chinese
Authors: W. Zhao, S.T. Ma and L.Q. Cui
It has been suggested that the angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism is linked to susceptibility to myocardial infarction (MI). In this study, we performed a meta-analysis to assess the relationship between ACE I/D polymorphism and MI in the Chinese Han population. Eight studies including a total of 1609 subjects w.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.15
- DOI:
- 10.4238/2015.July.17.15
Molecular identity of ramie germplasms using simple sequence repeat markers
Authors: M.B. Luan, B.F. Chen, Z.Z. Zou, J.J. Zhu, X.F. Wang, Y. Xu,Z.M. Sun and J.H. Chen
DNA identity is highly effective and efficient for distinguishing crop varieties regardless of their phenotypic similarities. To establish DNA identity in ramie, 21 simple sequence repeat primers were amplified in 108 accessions of domestic and exotic ramie germplasms. Sixty polymorphic bands were obtained, with an average of 2.9 bands per locus and 2-8 b.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.15
- DOI:
- 10.4238/2015.March.27.15
GSTT1, GSTM1, and GSTP1 polymorphisms and chemotherapy response in locally advanced breast cancer
Authors: A.L. Oliveira, F.F.O. Rodrigues, R.E. Santos, T. Aoki, M.N. Rocha, C.A. Longui and M.B. Melo
The glutathione S-transferase (GST) family consists of phase II detoxification enzymes that catalyze the conjugation of toxic substances, such as chemotherapeutic agents, to glutathione. We examined whether GSTT1/GSTT1“null”, GSTM1/GSTM1“null” and GSTP1Ile105Ile/GSTP1Ile105Val polymorphisms are associat.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr726
- DOI:
- 10.4238/vol9-2gmr726
Survival of the thriftiest: restricted nurture reveals the thrifty nature of a growth gene in Bos indicus
Authors: S.U. Dani, M.A.C. Dani, I.L. Freire, S.P. Gouvea, F.B. Knackfuss, F.P. Lima, M.E.Z. Mercadante, E. Monteiro, S.M.G. Paggiaro, A.G. Razook and H.C. Yehia
Growth hormone (GH) is a part of the somatotropic axis that controls metabolism, growth, development and aging in a wide range of animals. Mutations that reduce GH signaling have been associated with extended life spans and increased longevity in ways similar to what is observed in dietary restriction (DR) models. However, the.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr844
- DOI:
- 10.4238/vol9-2gmr844
Correlation between neuronal antibodies and limbic encephalitis in Chinese Han subjects
Authors: Q.M. Chen, H.D. Qu, W.D. Qian, L. Shen, L. Xu, Y.H. Chen,D.Q. Sang, L.N. Zhang, L. Yin, L. Li and H. Wang
A variety of anti-neuronal cell membrane antibodies such as voltage-gated potassium channel antibody, N-methyl-D-aspartate-2B-antibody, and glutamic acid decarboxylase antibody, are correlated with limbic encephalitis (LE). In this study on patients with LE, the clinical manifestations, psychology Wechsler Adult Intelligence Scale, cerebrospinal fluid, el.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.16
- DOI:
- 10.4238/2015.March.27.16
The synthesis of a new cassava-derived species, Manihot vieiri Nassar*
Authors: N.M.A. Nassar
A new species was synthesized artificially by chromosome doubling in a hybrid. The ensuing polyploid type exhibits an apomictic nature and maintains its morphological characteristics in the progeny. It showed a frequency of multiembryonic sacs of 29% in the ovules examined, whereas sacs were absent in the diploid type... Read More»
- Genet. Mol. Res. 5(3):