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Identification of SNPs located on BTA 6 and BTA 20 significantly associated with bovine respiratory disease in crossbred cattle
Authors: S.L. Miller, S. Mizell, R. Walker, T. Page and M.D. Garcia
The objective of the present study was to evaluate single nucleotide polymorphisms (SNPs) located in two quantitative trait locus (QTL) regions (BTA 6 and BTA 20) that are associated with bovine respiratory disease (BRD). A population of 323 crossbred steers sired by five purebred sire breeds during 2010-2013 (Angus, Braford, .. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048861
- DOI:
- 10.4238/gmr.15048861
High-resolution melting curve analysis of the ADSL and LPL genes and their correlation with meat quality and blood parameters in chickens
Authors: X.D. Zhang, Q.H. Li, L.F. Lou, J. Liu, X.H. Chen, C.X. Zhang and H.H. Wang
Adenylosuccinate lyase (ADSL) and lipoprotein lipase (LPL) are key enzymes in the metabolism of inosine monophosphate (IMP) and fat mass, which are important factors in meat quality evaluation. In this study, we selected 50 hens from the ISA B-line layers and Guangxi Yellow chickens, slaughtered the chickens at 120 days old, and analyzed polymorphisms in .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.13
- DOI:
- 10.4238/2015.March.20.13
Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes
Authors: H. Qiao, Y.W. Xiong, S.F. Jiang, H.T. Fu, S.M. Sun, S.B. Jin, Y.S. Gong and W.Y. Zhang
This study utilized high-throughput RNA sequencing technology to identify reproduction- and development-related genes of Macrobrachium nipponense by analyzing gene expression profiles of testis and ovary. More than 20 million 1 x 51-bp reads were obtained by Illumina sequencing, generating more than 7.7 and 11.7 million clean reads in the testis and ovary.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.14
- DOI:
- 10.4238/2015.March.20.14
Sequence variation and gene duplication at the MHC DRB loci of the spotted seal Phoca largha
Authors: X.G. Gao, J.B. Han, Z.C. Lu, P.J. Zhang and C.B. He
The major histocompatibility complex (MHC) is one of the most important genetic systems associated with resistance to infectious diseases in vertebrates. The spotted seal (Phoca largha) is one of the most endangered species in China. In this study, we present the first step in the molecular characterization of a DRB-like locus in the spotted seal by analy.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.15
- DOI:
- 10.4238/2015.March.20.15
Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis
Authors: E.C.B. Silva, M.A. Pelinca, A.C. Acosta, D.M.F. Silva, M.A. Gomes Filho and M.M.P. Guerra
Successful DNA extraction is indispensable for molecular methods based on polymerase chain reaction (PCR); however, goat sperm DNA extraction is limited. Thus, the aim of this study was to evaluate three methods to extract DNA from goat sperm for use in PCR. Eight goat semen pools were used for DNA extraction by using the DNea.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.21
- DOI:
- 10.4238/2014.August.7.21
Study of optimal scheme of spinal image-guided radiotherapy based on expression of caspase-3 in spinal cord neurons by orthogonal design
Authors: W.L. Xu, J.L.T. Tayerjiang, X.B. Zhao, H. Wang, Q. Wang and H. Yuan
The aim of the study was to optimize the biological safety scheme of spinal image-guided radiotherapy (IGRT) by determining the expression of caspase-3 in spinal cord neurons after IGRT. Thirty-six adult male beagles were assigned according to a random number table and subjected to IGRT to the 7th-12th canine thoracic vertebral bodies under a total dose o.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.13.1
- DOI:
- http://dx.doi.org/10.4238/2015.April.13.1
Traditional risk factors and angiotensin-converting enzyme insertion/deletion gene polymorphism in coronary artery disease
Authors: S. Sahin, K. Ceyhan, I. Benli, H. Ozyurt, E. Naseri, M.M. Tumuklu, L. Aydogan, A.O. Elalmis, A.F. Ozugurlu and O. Onalan
We investigated whether the insertion/deletion (I/D) polymorphism in the angiotensin-converting enzyme (ACE) gene and serum ACE levels are associated with traditional risk factors of coronary artery disease (CAD). We enrolled 250 individuals without CAD and 750 individuals suffering from CAD who were angiographically diagnosed. Biochemical risk factors, t.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.16
- DOI:
- 10.4238/2015.March.20.16
Characterization and comparison of flower bud microRNAs from yellow-horn species
Authors: Y. Ao
An increasing number of microRNAs (miRNAs) are known to play crucial regulatory roles in floral organ differentiation and development. Yellow-horn (Xanthoceras sorbifolia Bunge) is an oil-rich seed shrub that has great potential for biodiesel production. To evaluate the roles of miRNAs in the regulation of flower development, .. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048899
- DOI:
- 10.4238/gmr.15048899
Novel zinc protease gene isolated from Dictyostelium discoideum is structurally related to mammalian leukotriene A4 hydrolase
Authors: D. Fan and L.S. Hou
The allantoicase (allC) gene of Dictyostelium discoideum allC RNAi mutant strain was silenced using the RNA interference technique. The mutant strain is motile, aggregated, and could not undergo further morphological development. The growth rate is high and the cells show a shortened cell cycle comparing with wild-type D. discoideum. However, the mechanis.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Protein-protein interaction network and mechanism analysis of hepatitis C
Authors: Y. Tang, Q. Tang, C. Dong, X. Li, Z. Zhang and F. An
We predicted potential genes and identified pathways associated with hepatitis C. The gene expression profiles of GSE40184 from blood samples and GSE38597 from liver biopsy samples were downloaded from the GEO database. Differentially expressed genes (DEGs) were recognized using the Limma Package. The Pearson correlation test was used to construct the co-.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.17
- DOI:
- 10.4238/2015.March.20.17