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Alteration of coenzyme specificity of malate dehydrogenase from Streptomyces coelicolor A3(2) by site-directed mutagenesis
Authors: Y.D. Ge, P. Song, Z.Y. Cao, P. Wang and G.P. Zhu
We describe here for the first time the alteration of coenzyme specificity of malate dehydrogenase (MDH) from Streptomyces coelicolor A3(2) (ScMDH). In the present study, we replaced four amino acid residues in the Rossmann fold (βB-αC) region of NADH-dependent ScMDH by site-directed mutagenesis with those of NADPH-dependent MDH (Glu42.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.29.3
- DOI:
- 10.4238/2014.July.29.3
Population genetic structure of the tropical tree species Aegiphila sellowiana (Lamiaceae)
Authors: C. Medri, E.A. Ruas, C.F. Ruas, P.S. Medri, M.E. Medri and P.M. Ruas
The Tibagi River, located in southern Brazil, is associated with a significant degree of environmental heterogeneity, along its 550 km extension. There is concern about the integrity of this river’s ecosystem, as human interference has been increasing. Aegiphila sellowiana (Lamiaceae) is an important pioneer tree species.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.20.3
- DOI:
- 10.4238/2011.December.20.3
Molecular cloning and characterization of gene coding for �³-tocopherol methyltransferase from lettuce (Lactuca sativa)
Authors: Y.L. Tang, W.W. Ren, L. Zhang and K.X. Tang
γ-tocopherol methyltransferase is an important rate-limiting enzyme involved in tocopherol biosynthesis. The full-length cDNA encoding γ-tocopherol methyltransferase (designated as LsTMT) was cloned from Lactuca sativa for the first time by rapid amplification of cDNA ends and characterized by means of quantitative.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.21.2
- DOI:
- 10.4238/2011.December.21.2
Isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source
Authors: L.A. Cassa-Barbosa, R.E.L. Proc�³pio, I.T.S.R. Matos and S.A. Filho
Few yeasts have shown the potential to efficiently utilize hemicellulosic hydrolyzate as the carbon source. In this study, microorganisms isolated from the Manaus region in Amazonas, Brazil, were characterized based on their utilization of the pentoses, xylose, and arabinose. The yeasts that showed a potential to assimilate these sugars were selected for .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.12
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.12
Analysis of the association between polymorphisms in the vitamin D receptor (VDR) gene and dental caries in a Chinese population
Authors: X.P. Hu, Z.Q. Li, J.Y. Zhou, Z.H. Yu, J.M. Zhang and M.L. Guo
Environmental influences on the development and progression of dental caries are well known; however, there is little evidence of a genetic component imparting susceptibility to dental caries. The aim of this study was to investigate the relationship between a single nucleotide polymorphism in the vitamin D receptor TaqI locus and dental caries susceptibi.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.15
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.15
Correlation among RKIP expression, NF-�ºB p65 levels, and T-lymphocyte subsets in gastric cardia adenocarcinoma
Authors: H. Wei1, H.Q. Gao, H.B. Li, S.J. Qi1, W.L. Liu, L. Xu, H. Li, J.X. Liu and Z.M. Dong
The aim of this study was to characterize variations in Raf kinase inhibitor protein (RKIP) expression and related signaling molecules in gastric cardia adenocarcinoma. Cancerous and precancerous tissues were collected from patients with gastric cardia adenocarcinoma and normal tissue was collected from healthy controls. RKIP expression was detected in th.. Read More»
- Genet. Mol. Res. 14(4):
Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers
Authors: G.F. Cui*, L.F. Wu*, X.N. Wang, W.J. Jia, Q. Duan, L.L. Ma, Y.L. Jiang and J.H. Wang
Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average of 13 bands were amplified by each primer. Two software packages, POPGENE 1.32 and NTSYSpc 2.1, were used to analyze the.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.29.5
- DOI:
- 10.4238/2014.July.29.5
Forkhead box protein O1 mediates apoptosis in a cancer cervical cell line treated with the antitumor agent tumor necrosis factor-�±
Authors: B. Zhang, L. Gui, L. Zhu, X. Zhao, Y. Yang and Q. Li
Tumor necrosis factor-α (TNF-α) is an important pro-apoptotic cytokine, which performs a broad range of immune and inflammatory functions in several vital processes. TNF-α-induced apoptosis has been confirmed, however, relatively little is known regarding the role of forkhead box class-O 1 (FOXO1) in mediating TNF-α-induced apoptos.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.20
- DOI:
- 10.4238/2015.July.3.20
Optimization of chloroplast microsatellite PCR conditions and primer screening for endangered Rheum officinale, Rheum palmatum, and Rheum tanguticum
Authors: Y. Zhou, Z.J. Guo, L. Han, Y. Li and X.M. Wang
Chloroplast microsatellite primers were developed in order to provide more population genetic information of endangered Rheum officinale, R. palmatum, and R. tanguticum for conservation. The dried roots and rhizomes of these plants are important in traditional Chinese medicine. The results showed that the optimum concentrations .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.29.6
- DOI:
- 10.4238/2014.July.29.6
Development of SRS.php, a Simple Object Access Protocol-based library for data acquisition from integrated biological databases
Authors: Development of SRS.php, a Simple Object Access Protocol-based library for data acquisition from integrated biological databases
Data integration has become an important task for biological database providers. The current model for data exchange among different sources simplifies the manner that distinct information is accessed by users. The evolution of data representation from HTML to XML enabled programs, instead of humans, to interact with biologica.. Read More»
- Genet. Mol. Res. 6(4):