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Development of polymorphic expressed sequence tag-single sequence repeat markers in the common Chinese cuttlefish, Sepiella maindroni
Authors: R.H. Li, S.K. Lu, C.L. Zhang, W.W. Song, C.K. Mu and C.L. Wang
The common Chinese cuttlefish (Sepiella maindroni) is one of the popular edible cephalopod consumed across Asia. To facilitate the population genetic investigation of this species, we developed fourteen polymorphic microsatellite makers from expressed sequence tags of S. maindroni. The number of alleles at ea.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.3
- DOI:
- 10.4238/2014.July.25.3
Genetic diversity of worldwide Jerusalem artichoke (Helianthus tuberosus) germplasm as revealed by RAPD markers
Authors: P.P. Wangsomnuk, S. Khampa, P. Wangsomnuk, S. Jogloy, T. Mornkham, B. Ruttawat, A. Patanothai and Y.B. Fu
Jerusalem artichoke (Helianthus tuberosus) is a wild relative of the cultivated sunflower (H. annuus); it is an old tuber crop that has recently received renewed interest. We used RAPD markers to characterize 147 Jerusalem artichoke accessions from nine countries. Thirty RAPD primers were screened; 13 of them detected 357 repr.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.12.4
- DOI:
- 10.4238/2011.December.12.4
Regulatory effect of iron regulatory protein-2 on iron metabolism in lung cancer
Authors: Z. Cheng, L.L. Dai, Y.N. Song, Y. Kang, J.M. Si, J. Xia and Y.F. Liu
Iron metabolism plays an important role in the pathogenesis of lung cancer. This study aimed to investigate the effect of gene silencing of iron regulatory protein-2 (IRP2) on mRNA and protein expression of transferrin (Tf), transferrin receptor (TfR), and ferritin (Fn) in A549 lung cancer cells. A549 cells were cultured and d.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.5
- DOI:
- 10.4238/2014.July.25.5
Treatment of seawater immersion-complicated open-knee joint fracture
Authors: J.G. Ai, F. Zhao, Z.M. Gao, W. Dai, L. Zhang, H.B. Chen and J.G. Zhou
The current study aimed to select suitable remedies for seawater immersion-complicated open-knee joint fracture by exploring the effects of different treatment methods. Forty adult rabbits weighing 2.20 ± 0.25 kg were divided equally into internal fracture fixation group (A), seawater-immersed group with primary interna.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.6
- DOI:
- 10.4238/2014.July.25.6
Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing
Authors: H. Shen, H.Y. Chen, B. Jia, G.H. Han, Y.S. Zhang and X.C. Zeng
The role of microRNAs (miRNAs) in the regulation of mammalian reproduction has been demonstrated previously. However, only a few studies have assessed the role of miRNAs in the reproduction processes of sheep. The elucidation of miRNA expression profiles in the ovaries of different sheep breeds representing fecundity extremes will be useful in understandi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.11
- DOI:
- 10.4238/2015.July.3.11
Development of eight novel microsatellite markers for Huoyan geese
Authors: Z.Z. Cao, D. Su, Y.Y. Zhao, M. Liu, M. Gao and X.H. Luan
In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers we.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.10
- DOI:
- 10.4238/2014.July.25.10
Temporal and tissue expression of genes involved in buds of earliness cotton cultivar
Authors: V.G.L. Batista, M.P.N. Pinheiro, P.A. Melo Filho, R.C. Santos and L.M. Lima
Gene sequences previously identified in Arabidopsis buds were used as references in order to estimate temporal and tissue expression in buds, leaves, stem, and root tissues in cotton plants. Buds were evaluated during 3 phases: 2-8, 10-12, and 14-20 mm. Primers were designed for the ARF6, ATFY, and SEUSS genes for use in semi-quantitative reverse transcri.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.14
- DOI:
- 10.4238/2015.July.3.14
Genetic and environmental factors that influence production and quality of milk of Alpine and Saanen goats
Authors: L.F. Brito, F.G. Silva, A.L.P. Melo, G.C. Caetano, R.A. Torres, M.T. Rodrigues and G.R.O. Menezes
Data from 1279 lactations of 783 Alpine and Saanen goats of the herd of our university in Minas Gerais, Brazil, were used to study environmental effects on and to estimate genetic parameters for milk production until 270 days of lactation (MP270) and for production and percentages of fat (PFAT and %FAT), protein (PPROT and %PR.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.14.9
- DOI:
- 10.4238/2011.December.14.9
Treatment of moyamoya disease by multipoint skull drilling for indirect revascularization combined with mobilization of autologous bone marrow stem cells
Authors: R. Wu, N. Su, Z. Zhang and F. Jia
This study discusses the clinical efficacy of multipoint skull drilling for indirect revascularization combined with mobilization of autologous bone marrow stem cells and use of simvastatin in the treatment of moyamoya disease. Seventy-eight patients [control group (group A), 39 patients; experimental group (group B), 39 patients] with moyamoya disease we.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.27
- DOI:
- 10.4238/2015.July.3.27
Key pathways involved in prostate cancer based on gene set enrichment analysis and meta analysis
Authors: Q.Y. Ning, J.Z. Wu, N. Zang, J. Liang, Y.L. Hu and Z.N. Mo
Prostate cancer is one of the most common male malignant neoplasms; however, its causes are not completely understood. A few recent studies have used gene expression profiling of prostate cancer to identify differentially expressed genes and possible relevant pathways. However, few studies have examined the genetic mechanics o.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.14.10
- DOI:
- 10.4238/2011.December.14.10