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Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae)
Authors: M.A. Lopes, B.T. Hora Junior, C.V. Dias, G.C. Santos, K.P. Gramacho, J.C.M. Cascardo, A.S. Gesteira and F. Micheli
Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches’ broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused o.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr825
- DOI:
- 10.4238/vol9-3gmr825
Linkage of schizophrenia with TPH2 and 5-HTR2A gene polymorphisms in the Malay population
Authors: S.F. Tee, T.J. Chow, P.Y. Tang and H.C. Loh
The serotoninergic system has been implicated in the etiology of schizophrenia and other behavioral disorders. Association studies have focused on the tryptophan hydroxylase 2 gene (TPH2) and the 5-hydroxytryptamine receptor 2A gene (5-HTR2A). We genotyped two single-nucleotide polymorphisms, A1438G of 5-HTR2A and intronic rs1.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr789
- DOI:
- 10.4238/vol9-3gmr789
Citrullinemia type I: molecular screening of the ASS1 gene by exonic sequencing and targeted mutation analysis
Authors: R. Marquis-Nicholson, E. Glamuzina, D. Prosser, C. Wilson and D.R. Love
We developed a mutation-screening protocol for the ASS1 gene in order to guide clinical management of neonates with elevated citrulline detected during routine newborn screening. An exon-based amplification and sequencing method was designed and successfully applied to patients to identify disease-associated mutations. The seq.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr834
- DOI:
- 10.4238/vol9-3gmr834
Genetically modified animals for use in research and biotechnology
Authors: L.M. Chaible, M.A. Corat, E. Abdelhay and M.L.Z. Dagli
Transgenic animals are used extensively in the study of in vivo gene function, as models for human diseases and in the production of biopharmaceuticals. The technology behind obtaining these animals involves molecular biology techniques, cell culture and embryo manipulation; the mouse is the species most widely used as an expe.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr867
- DOI:
- 10.4238/vol9-3gmr867
Isolation of retro-transcribed RNA from in vitro Mycosphaerella fijiensis-infected banana leaves
Authors: C.M. RodrÃ?Âguez-GarcÃ?Âa, L. Peraza-EcheverrÃ?Âa, I.R. Islas-Flores, B.B. Canto-CanchÃ?© and R. Grijalva-Arango
High polyphenol and polysaccharide levels in plant tissues such as banana fruit and leaves constitute a significant challenge to the extraction of sufficient amounts of high-quality RNA required for cDNA library synthesis and molecular analysis. To determine their comparative effectiveness at eliminating polyphenols, polysacch.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr865
- DOI:
- 10.4238/vol9-3gmr865
Resistance of Castanea mollissima Shuhe-WYL strain to Dryocosmus kuriphilus and its molecular mechanism
Authors: G.M. Geng, C.C. Zhu and J.Y. Zhou
The resistance of Castanea mollissima Shuhe-WYL strain to Dryocosmus kuriphilus and its molecular mechanism were examined. The larvae of D. kuriphilus were inoculated on the Shuhe-WYL and Qingzha strains, and mortality was observed and compared; the relative mRNA content of the OsCDPK2, receptor-like protein, OsNAC6 protein, KH domain protein, RNA-binding.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.12
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.12
Establishment and rapid detection of a heterozygous missense mutation in the CACNA1F gene by ARMS technique with double-base mismatched primers
Authors: W.C. Yang, L. Zhu, B.X. Zhou, S. Tania, Q. Zhou, M.A. Khan,X.L. Fu, J.L. Cheng, H.B. Lv and J.J. Fu
Retinitis pigmentosa (RP) is a retinal degenerative disorder that often causes complete blindness. Mutations of more than 50 genes have been identified as associated with RP, including the CACNA1F gene. In a recent study, by employing next-generation sequencing, we identified a novel mutation in the CACNA1F gene. In this study, we used the amplification r.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.14
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.14
Correlation between polymorphisms in the IL-17A and IL-17F genes and development of coronary artery disease
Authors: G.Y. Geng, H.L. Liu, Y.J. Zhao, L. Wu, L. Mao and N. Ba
A case-control study was conducted to investigate the association between genetic variants of IL-17A rs2275913 and IL-17F rs763780 and the development of coronary artery disease (CAD) in a Chinese population. A total of 306 individuals with CAD and 306 unaffected individuals were enrolled from the Zhengzhou People’s Hospital between May 2012 and May.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.15
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.15
Genetic diversity and genetic structure of consecutive breeding generations of golden mandarin fish (Siniperca scherzeri Steindachner) using microsatellite markers
Authors: X.N. Luo, M. Yang, X.F. Liang, K. Jin, L.Y. Lv, C.X. Tian, Y.C. Yuan and J. Sun
In this study, 12 polymorphic microsatellites were investigated to determine the genetic diversity and structure of 5 consecutive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breed.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.1
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.1
Effects of glucose and disorders in lipid metabolism on cytokine levels and cognitive impairment in the olanzapine-induced obesity rat model
Authors: X.J. Ma, T.X. Maimaitirexiati, R. Zhang, G. Hu and X. Luo
The aim of the study was to explore the effects of increased levels of blood sugar and cytokines on impaired cognitive function in the olanzapine-induced obesity rat model. A total of 40 rats were randomly divided into 2 groups; the control and olanzapine groups (N = 20 per group). The control rats were fed regular food, while the olanzapine rats received.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.7
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.7