Abstract

Establishment and rapid detection of a heterozygous missense mutation in the CACNA1F gene by ARMS technique with double-base mismatched primers

Author(s): W.C. Yang, L. Zhu, B.X. Zhou, S. Tania, Q. Zhou, M.A. Khan,X.L. Fu, J.L. Cheng, H.B. Lv and J.J. Fu

Retinitis pigmentosa (RP) is a retinal degenerative disorder that often causes complete blindness. Mutations of more than 50 genes have been identified as associated with RP, including the CACNA1F gene. In a recent study, by employing next-generation sequencing, we identified a novel mutation in the CACNA1F gene. In this study, we used the amplification refractory mutation system (ARMS) and identified a single nucleotide change c.1555C>T in exon 13 of the CACNA1F gene, leading to the substitution of arginine by tryptophan (p.R519W) in a Chinese individual affected by RP.This study actually confirms this novel mutation, and establishes the ARMS technique for the detection of mutations in RP.

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