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Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction
Authors: F.A. Martins P.C.S. Carneiro C.T. Guimar?£es J.V. Magalh?£es J.E.S. Carneiro C.D. Cruz
The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, consideri.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr585
- DOI:
- 10.4238/vol8-1gmr585
Low-level sequence variation in Toxoplasma gondii calcium-dependent protein kinases among different genotypes
Authors: J.L. Wang, N.Z. Zhang, S.Y. Huang, Y. Xu1,2, R.A. Wang and X.Q. Zhu
The causative agent of toxoplasmosis, Toxoplasma gondii, can infect virtually all nucleated cell types of warm-blooded animals. In this study, we examined the sequence variation in calciumdependent protein kinase 2 (CDPK2) genes among 13 T. gondii strains from different hosts and geographical locations. The results showed that the lengths of the complete .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.28
- DOI:
- 10.4238/2015.May.11.28
Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots
Authors: C.R. de Souza F.J. Arag?£o E.C.O. Moreira C.N.M. Costa S.B. Nascimento L.J. Carvalho
Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be neces.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr560
- DOI:
- 10.4238/vol8-1gmr560
Molecular and chromosomal mutations among children with B-lineage lymphoblastic leukemia in Brazilâ??s Federal District
Authors: D.R. Mesquita J.C. C?³rdoba ?.Q. Magalh?£es M.S. C?³rdoba J.R.C. Oliveira A. Gon?§alves I. Ferrari C. Martins-de-S?¡
Acute lymphoblastic leukemia (ALL) accounts for approximately 80% of all acute leukemias during childhood. Chromosomal anomalies resulting from gene fusion, which are frequent in leukemias, create hybrid transcripts, the great majority of which encode transcription factors. We analyzed 88 pediatric patients (median age 7.3 yea.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr582
- DOI:
- 10.4238/vol8-1gmr582
Molecular cytogenetic characterization of a new leaf rolling triticale
Authors: E.N. Yang, Z.J. Yang, J.F. Zhang, Y.C. Zou and Z.L. Ren
Leaf rolling occurs in some cereal genotypes in response to drought. We identified and made a phenotypic, cytological and physiological analysis of a leaf-rolling genotype (CMH83) of hexaploid triticale (X Triticosecale Wittmack) that exhibited reduced plant height, rolled and narrow leaves. Gliadin electrophoresis of seed pro.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.29.6
- DOI:
- 10.4238/2011.November.29.6
Genome sequencing and systems biology analysis of a lipase-producing bacterial strain
Authors: N. Li*, D.D. Li*, Y.Z. Zhang, Y.Z. Yuan, H. Geng, L. Xiong and D.L. Liu
Lipase-producing bacteria are naturally-occurring, industrially-relevant microorganisms that produce lipases, which can be used to synthesize biodiesel from waste oils. The efficiency of lipase expression varies between various microbial strains. Therefore, strains that can produce lipases with high efficiency must be screened, and the conditions of lipas.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017331
- DOI:
- 10.4238/gmr.15017331
A diagnostic kit for the enteroviruses Coxsackie A6 and A10
Authors: X.Y. Zhang1, X. Yao2, Z.W. Chen3, X.H. Jiang4, N.H. Xie3, C.Y. Zhang5 and Z.H. Guan5
Recently, there has been an upward trend in the occurrence of hand-foot-mouth disease, which is correlated with Coxsackie A6 and A10 infections. Although two separate diagnostic reagents are available for these two viral strains, the protocol and diagnosis efficiency still need to be improved. More importantly, as co-infection with these viruses is common.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017431
- DOI:
- 10.4238/gmr.15017431
Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate
Authors: X.K. Xing, H.G. Feng and Z.Q. Yuan
This study investigated the efficacy and feasibility of inducing the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro using Sprague Dawley rats, as a model of hepatocyte generation for cell transplantation. BMSCs were isolated and grown using the adherent method and exposed to 5 or 10% liver tissue .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038550
- DOI:
- 10.4238/gmr.15038550
Molecular evolutionary analysis of the high-affinity K+ transporter gene family in angiosperms
Authors: P. Yang, C. Hua, F. Zhou, B.-J. Zhang, X-N. Cai, Q.-Z. Chen and R.-L. Wang
The high-affinity K+ transporter (HKT) family comprises a group of multifunctional cation transporters widely distributed in organisms ranging from Bacteria to Eukarya. In angiosperms, the HKT family consists primarily of nine types, whose evolutionary relationships are not fully understood. The available sequences from 31 plant species were used to perfo.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038781
- DOI:
- 10.4238/gmr.15038781
Protein tyrosine kinase regulates ?±-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking induced by acute hypoxia in cultured brainstem neurons
Authors: H. Wang, L.C. Yu and Y.C. Li
This study was performed to investigate the modulation effect of protein tyrosine kinase on postsynaptic a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking induced by acute hypoxia in cultured brainstem neurons. The cultured neurons were exposed to 1% O2 and the expression of AMPA receptor subunit GluR2 on the cell surface wa.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038797
- DOI:
- 10.4238/gmr.15038797