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Animal Genetics   Research Article

Regulation of zinc transporter 3 and carbonic anhydrases 2 and 14 mRNA expression in the retina of rats affected by low dietary zinc.

Authors: P.B. Zhang, H.Y. Xiao, H. Liu and R. Qin

We looked at how zinc transporter 3 ZnT-3) mRNA expression in the rat retina is affected by low dietary zinc. Groups of 12 four-week-old male Sprague Dawley rats were fed on a low-zinc diet for 2, 4 or 6 weeks. Half of each group was then fed with a normal-zinc content diet and the other half was given a low-zinc content diet... Read More»

Genet. Mol. Res. 13(1):
2014.February.19.7
DOI:
10.4238/2014.February.19.7
Animal Genetics   Research Article

Newly identified gene muscle segment homeobox C may play a role in intermuscular bone development of Hemibarbus labeo

Authors: Y.P. Lv, W.J. Yao, J. Chen and B.L. Bao

Intermuscular bones, ossified from tendons within the myosepta, occur only in teleost fish. Current understanding of the homology and origins of intermuscular bones in fishes is based mainly on morphological data. To date, there is no published data regarding molecular mechanisms of intermuscular bone formation. In this study, we cloned the gene muscle se.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.22.26
DOI:
http://dx.doi.org/10.4238/2015.September.22.26
Plant Genetics   Research Article

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Authors: S.S. Ahmed, Z.-H. Gong, M.A. Khan, Y.-X. Yin, W.-L. Guo and J. Imran

Activity and expression of polygalacturonase (PG), a hydrolytic enzyme involved in ultrastructural changes in the pericarp of sweet pepper (Capsicum annaum), were investigated at different ripening stages of the pepper cultivars Mandi and Talanduo. Molecular cloning of CaPG was carried out by constructing a cDNA library from t.. Read More»

Genet. Mol. Res. 10(4):
2011.November.22.10
DOI:
10.4238/2011.November.22.10
Plant Genetics   Research Article

Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior

Authors: M.M.U. Castanhole, L.L.V. Pereira, H.V. Souza, H.E.M.C. Bicudo, L.A.A. Costa and M.M. Itoyama

Males of Limnogonus aduncus were found to have the sex chromosome system X0 and chromosome number 2n = 23 (22A + X0). Testis cells were stained with lacto-acetic orcein and silver nitrate so that changes in the morphology and degree of staining of the heteropicnotic chromatin and the nucleolar material could be observed during meiosis and spermiogenesis. .. Read More»

Genet. Mol. Res. 7(4):
vol7-4gmr527
DOI:
10.4238/vol7-4gmr527
Animal Genetics   Research Article

Optimizing reproducibility evaluation for random amplified polymorphic DNA markers

Authors: J.R. Ramos, M.P.C. Telles, J.A.F. Diniz-Filho, T.N. Soares, D.B. Melo and G. Oliveira

The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice f.. Read More»

Genet. Mol. Res. 7(4):
vol7-4gmr520
DOI:
10.4238/vol7-4gmr520
Animal Genetics   Research Article

High genetic differentiation of Aegla longirostri (Crustacea, Decapoda, Anomura) populations in southern Brazil revealed by multi-loci microsatellite analysis

Authors: M.L. Bartholomei-Santos, P.A. Roratto and S. Santos

Species with a broad distribution rarely have the same genetic make-up throughout their entire range. In some cases, they may constitute a cryptic complex consisting of a few species, each with a narrow distribution, instead of a single-, widely distributed species. These differences can have profound impacts for biodiversity .. Read More»

Genet. Mol. Res. 10(4):
2011.November.22.4
DOI:
10.4238/2011.November.22.4
Plant Genetics   Research Article

Identification and functional characterization of esterases in Euschistus heros (Hemiptera, Pentatomidae) and their relationship with thiamethoxam and lambda-cyhalothrin

Authors: L.A. Hegeto, L. Ronqui, A.S. Lapenta and F.A. Albuquerque

The brown stink bug Euschistus heros is the most abundant species of the soybean-sucking bugs, and causes large economic losses. Applying different chemical groups of organosynthetic insecticides for its control increases the potential for resistance. Esterases are a group of enzymes that play a variety of roles in insects, and some of them are related to.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.22.1
DOI:
http://dx.doi.org/10.4238/2015.September.22.1
Animal Genetics   Research Article

Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona

Authors: M.V.C. Viana, E.A. Miranda, A.K. de Francisco, C.A.L. Carvalho and A.M. Waldschmidt

Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intrac.. Read More»

Genet. Mol. Res. 10(4):
2011.November.22.11
DOI:
10.4238/2011.November.22.11
Medical Genetics   Research Article

Novel NKX2-5 mutations responsible for congenital heart disease

Authors: J. Wang, X.Y. Liu and Y.Q. Yang

Congenital heart disease (CHD) is the most common birth defect and is the leading cause of infant morbidity and mortality resulting from birth defects. Increasing evidence demonstrates that genetic variation in the NKX2-5 gene, which encodes a homeobox-containing transcription factor crucial to cardiogenesis, is an important m.. Read More»

Genet. Mol. Res. 10(4):
2011.November.29.1
DOI:
10.4238/2011.November.29.1
Human Genetics   Research Article

Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals

Authors: C.J. Lindsey M.E. Almeida C.F. Vicari C. Carvalho A. Yaguiu A.C. Freitas W. Be�§ak R.C. Stocco

Papillomavirus infection in bovines is associated with cutaneous papillomatosis on the hide, udders and other epithelial tissues, as well as in oral respiratory, alimentary and urinary tract mucosa. Bovine papillomavirus (BPV) is also considered the etiological agent of esophageal tumors and the malignant bladder tumors that c.. Read More»

Genet. Mol. Res. 8(1):
vol8-1gmr573
DOI:
10.4238/vol8-1gmr573