
Authors: P.B. Zhang, H.Y. Xiao, H. Liu and R. Qin
We looked at how zinc transporter 3 ZnT-3) mRNA expression in the rat retina is affected by low dietary zinc. Groups of 12 four-week-old male Sprague Dawley rats were fed on a low-zinc diet for 2, 4 or 6 weeks. Half of each group was then fed with a normal-zinc content diet and the other half was given a low-zinc content diet... Read More»
Authors: Y.P. Lv, W.J. Yao, J. Chen and B.L. Bao
Intermuscular bones, ossified from tendons within the myosepta, occur only in teleost fish. Current understanding of the homology and origins of intermuscular bones in fishes is based mainly on morphological data. To date, there is no published data regarding molecular mechanisms of intermuscular bone formation. In this study, we cloned the gene muscle se.. Read More»
Authors: S.S. Ahmed, Z.-H. Gong, M.A. Khan, Y.-X. Yin, W.-L. Guo and J. Imran
Activity and expression of polygalacturonase (PG), a hydrolytic enzyme involved in ultrastructural changes in the pericarp of sweet pepper (Capsicum annaum), were investigated at different ripening stages of the pepper cultivars Mandi and Talanduo. Molecular cloning of CaPG was carried out by constructing a cDNA library from t.. Read More»
Authors: M.M.U. Castanhole, L.L.V. Pereira, H.V. Souza, H.E.M.C. Bicudo, L.A.A. Costa and M.M. Itoyama
Males of Limnogonus aduncus were found to have the sex chromosome system X0 and chromosome number 2n = 23 (22A + X0). Testis cells were stained with lacto-acetic orcein and silver nitrate so that changes in the morphology and degree of staining of the heteropicnotic chromatin and the nucleolar material could be observed during meiosis and spermiogenesis. .. Read More»
Authors: J.R. Ramos, M.P.C. Telles, J.A.F. Diniz-Filho, T.N. Soares, D.B. Melo and G. Oliveira
The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice f.. Read More»
Authors: M.L. Bartholomei-Santos, P.A. Roratto and S. Santos
Species with a broad distribution rarely have the same genetic make-up throughout their entire range. In some cases, they may constitute a cryptic complex consisting of a few species, each with a narrow distribution, instead of a single-, widely distributed species. These differences can have profound impacts for biodiversity .. Read More»
Authors: L.A. Hegeto, L. Ronqui, A.S. Lapenta and F.A. Albuquerque
The brown stink bug Euschistus heros is the most abundant species of the soybean-sucking bugs, and causes large economic losses. Applying different chemical groups of organosynthetic insecticides for its control increases the potential for resistance. Esterases are a group of enzymes that play a variety of roles in insects, and some of them are related to.. Read More»
Authors: M.V.C. Viana, E.A. Miranda, A.K. de Francisco, C.A.L. Carvalho and A.M. Waldschmidt
Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intrac.. Read More»
Authors: J. Wang, X.Y. Liu and Y.Q. Yang
Congenital heart disease (CHD) is the most common birth defect and is the leading cause of infant morbidity and mortality resulting from birth defects. Increasing evidence demonstrates that genetic variation in the NKX2-5 gene, which encodes a homeobox-containing transcription factor crucial to cardiogenesis, is an important m.. Read More»
Authors: C.J. Lindsey M.E. Almeida C.F. Vicari C. Carvalho A. Yaguiu A.C. Freitas W. Be�§ak R.C. Stocco
Papillomavirus infection in bovines is associated with cutaneous papillomatosis on the hide, udders and other epithelial tissues, as well as in oral respiratory, alimentary and urinary tract mucosa. Bovine papillomavirus (BPV) is also considered the etiological agent of esophageal tumors and the malignant bladder tumors that c.. Read More»